The prognostic value of leucine-rich repeat-containing G-protein (Lgr5) and its impact on clinicopathological features of colorectal cancer

Abstract

Introduction Colorectal cancer (CRC) constitutes one of the most prevalent malignancies in the world. Recent research suggests that cancer stem cells (CSCs) are responsible for tumor cell's malignant behavior in CRC. This study has been designed to determinate clinical implications of CSC markers: CD44, DCLK1, Lgr5, and ANXA2 in CRC. Materials and methods The study was performed on tissue samples which were collected from 89 patients undergoing colectomy. Formalin-fixed paraffin-embedded tissue blocks with representative tumor areas were identified and corded. Immunohistochemical staining was performed using anti-CD44, anti-LGR5, anti-ANXA2, and anti-DCLK1 antibodies. The H-score system was utilized to determine the immunointensity of CRC cells. Results The lower expression of Lgr5 was significantly correlated with the presence of lymph-node metastases (p = 0.011), while high expression of Lgr5 was statistically significant in vascular invasion in examined cancer tissue samples (p = 0.027). Moreover, a high H-score value of Lgr5 expression was significantly related to a reduced overall survival rate (p = 0.043). Conclusion Our results suggest a strong relationship between CSC marker Lgr5 and vascular invasion, presence of lymphnode metastasis, and overall poor survival. The presence of Lgr5 might be an unfavorable prognostic factor, and its high level in cancer tissue is related to an aggressive course. This marker could also be used to access the effectiveness of the treatment.

Prognostic Impact and Functional Annotations of KIF11 and KIF14 Expression in Patients with Colorectal Cancer

“…The clinical stages of all CRC tumors were reexamined according to the TNM 8th edition classification of the American Joint Committee on Cancer (AJCC) criteria [ 62 ]. A part of this cohort was previously described [ 63 ]. The study protocol was approved by the Bioethics Committee at Collegium Medicum in Bydgoszcz of Nicolaus Copernicus University in Toruń (no.…”

“…Tissue microarrays (TMAs) construction and then immunohistochemical staining of slides with tissuecores were performed as previously described [ 63 ]. For the majority of cases, four 2.0 mm cores were sampled from different tumor areas.…”

Correlation between levels of LGR-5 (leucine rich repeat containing protein coupled receptor 5) with clinical aspects and colorectal carcinoma stage

“…Excess LGR-5 levels were significantly associated with deep invasion (P = 0.002), lymph node metastasis (P = 0.003), distant metastasis (P = 0.001), and AJCC stage (P = 0.01); but not correlated with tumor grading (P = 0.433) (Jiang et al, 2016). Several variables in other studies also showed a positive correlation of LGR-5 levels, such as that of Gzil et al, who showed lower LGR-5 levels were significantly correlated with the presence of lymph node metastases (p = 0.011), while LGR levels The high -5 was statistically significant in vascular invasion (p = 0.027) (Gzil et al, 2020).…”

Diagnostic significance and carcinogenic mechanism of pan‐cancer gene POU5F1 in liver hepatocellular carcinoma

“…Many studies have certified that cancer stem cells (CSCs) are associated with aggression, metastasis, and recrudescence in various cancers. In addition, several CSC markers have been proven to contribute to the poor prognosis of cancers, [4][5][6][7] indicating the significance of CSC markers in the prognosis of malignancies. However, due to the complexity of the regulatory network in tumor pathologic processes, the prognostic significance of CSC markers is not fully understood.…”