The proinflammatory cytokines TNF-α and IL-6 in lumpfish (Cyclopterus lumpus L.) -identification, molecular characterization, phylogeny and gene expression analyses

Eggestøl, Håvard Øritsland; Lunde, Harald S.; Haugland, Gyri Teien

doi:10.1016/j.dci.2020.103608

Cited by 24 publications

(11 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent reports indicated that TNFα was also involved in driving cytokine storm and cell death associated with COVID-19 (Karki et al, 2021). Although some isoforms of TNF family have been identified in fish (Eggestol et al, 2020;Grayfer et al, 2008 In this study, a TNFα homolog gene from pufferfish was investigated especially its role in pathogen infection and induced apoptosis.…”

Section: Discussionmentioning

confidence: 98%

“…Recent reports indicated that TNF‐ α was also involved in driving cytokine storm and cell death associated with COVID‐19 (Karki et al., 2021). Although some isoforms of TNF family have been identified in fish (Eggestol et al., 2020; Grayfer et al., 2008; Hirono et al., 2000; Huang et al., 2020; Savan et al., 2005), the researches on the biological functions are still limited, such as the role of TNF‐ α in pathogen infection and apoptosis has not been reported yet in fish. In this study, a TNF‐ α homolog gene from pufferfish was investigated especially its role in pathogen infection and induced apoptosis.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Functional characterization of TNF‐α in pufferfish (Takifugu obscurus) in immune response and apoptosis against Aeromonas hydrophila

Kong

Qian

et al. 2021

Journal of Fish Diseases

View full text Add to dashboard Cite

Tumour necrosis factor‐α (TNF‐α) is a multifunctional cytokine involved in immune system homeostasis, antimicrobial defence, regulation of apoptosis, cell proliferation and differentiation. Although the pro‐inflammatory property of TNF‐α has been made new progress, detailed research on host defence against bacterial infection and inducing apoptosis remains to be revealed in early vertebrates. Here, we reported the TNF‐α homologue (ToTNF‐α) from pufferfish (Takifugu obscurus). The open reading frame (ORF) of ToTNF‐α was 753 bp, encoding a protein of 250 aa contained the TNF family signature and conserved cysteine residues. The mRNA expression of ToTNF‐α had a wide range of tested tissues, with the highest expression in the skin. After Aeromonas hydrophila infection, the mRNA expression of ToTNF‐α was significantly up‐regulated both in vivo and in vitro experiments. After stimulation by recombinant protein of ToTNF‐α ((r)ToTNF‐α), the relative expressions of endogenous TNF‐α, caspase 8, caspase 3, p53, and Bax inhibitor‐1 in head kidney leucocytes were all notably up‐regulated. These results showed that ToTNF‐α might induce apoptosis depend on pro‐ and anti‐apoptotic proteins at mRNA level. Moreover, flow cytometry analysis indicated that the (r)ToTNF‐α can induce apoptosis of head kidney leucocytes. Taken together, these characteristics suggest that ToTNF‐α can participate in immune response against A. hydrophila and induce apoptosis at mRNA and cellular level, which will help to understand the mechanism of apoptosis and immune response in teleost fish.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Functional characterization of TNF‐α in pufferfish (Takifugu obscurus) in immune response and apoptosis against Aeromonas hydrophila

Kong

Qian

et al. 2021

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…FKN/CX3CR1 plays an important role in the development of neuroinflammation and neuronal damage in hippocampus. FKN/ CX (3) CR1 signaling in young adult rodents decreased survival and proliferation of neural progenitor cells through IL-1β [27], also leading to the reduction of proin ammatory cytokines TNF-α and IL-6 [28]. e MWM is widely used for the functional evaluation of brain regions related to spatial learning and memory and the open eld test is a simple method to assess the locomotor activity of rats based on their innate tendency to avoid open spaces [29].…”

Section: Discussionmentioning

confidence: 99%

FKN/NR Signaling Pathway Regulates Hippocampal Inflammatory Responses: the Survival of Hippocampal Neurons in Diabetic Rats with Chronic Unpredictable Mild Stress

Liu

Zhao

Liu

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Aim. To investigate the mechanism via which FKN/CX3CR1 signaling abnormalities mediate N-methyl-D-aspartic acid receptor (NMDA) overexcitation-induced hippocampal neuronal injury in diabetic rats complicated with depression (DD). Methods. Sixty rats were randomly divided into 5 groups. The depression-like behaviors of the rats were evaluated by open field test and Morris water maze. The pathological changes of hippocampus in DD rats were observed by HE staining. The blood levels of inflammatory factors (IL-1β, TNF-α, and IL-6) and neurotransmitters (D-serine and glutamic acid) were determined by enzyme-linked immunosorbent assay (ELISA). The expressions of BDNF, A1 receptor (A1R), A2 receptor (A2R), A3 receptor (A3R), calmodulin dependent kinase II (CaMKII), CX3CR1, CX3CL1 (FKN), NR2A, and NR2B proteins were detected by immunohistochemistry and Western-blotting. Results. Compared with the normal control group, blood glucose level increased significantly and body weight decreased in T2DM group and T2DMC group. In addition, the number of spontaneous activities significantly decreased and the capability of learning and memory was attenuated in T2DMC group and Chronic Stress group. The blood levels of IL-1β, TNF-α, IL-6, glutamate (Glu), and D-serine significantly increased in each model group. After intervention with CX3CR1 antibody, the expressions of BDNF, CaMK II, A1R, and A3R increased and those of A2R, CX3CR1, FKN, NR2A, and NR2B decreased. Conclusion. In the diabetic state, the binding of FKN to CX3CR1 increases, which regulates a variety of adenosine receptors. When it exerts its effect on neurons, the overactivation of NR results in neuronal injury and causes depression.

show abstract

“…A section of the medulla oblongata constituted the brain sample. Homogenization of the tissue-samples was performed as described previously (26). Briefly, up to 40 mg of tissue were transferred to FastPrep Tubes containing SS metal beads lysing matrix (MP biomedicals) and lysisbuffer and homogenized in a FastPrep-24 5G homogenizator (MP biomedicals).…”

Section: Tissue Sampling and Homogenizationmentioning

confidence: 99%

Interleukin-1 Ligands and Receptors in Lumpfish (Cyclopterus lumpus L.): Molecular Characterization, Phylogeny, Gene Expression, and Transcriptome Analyses

et al. 2020

Self Cite

View full text Add to dashboard Cite

The interleukin (IL)-1 family play a fundamental role as immune system modulators. Our previous transcriptome-analyses of leukocytes from lumpfish (Cyclopterus lumpus L.) showed that IL-1β was among the most highly upregulated genes following bacterial exposure. In the present study, we characterized IL-1 signaling pathways, identified and characterized four ligands of the IL-1 family in lumpfish; IL-1β type I and type II, IL-18, and the novel IL-1 family members (nIL-1F), both at mRNA and gene levels. The two IL-1β in lumpfish is termed IL-1β1 (type II) and IL-1β2 (type I). Furthermore, a comprehensive phylogenetic analysis of 277 IL-1 ligands showed that nIL-1F, in common with IL-1β, likely represents an ancestral gene, as representatives for nIL-1F were found in cartilaginous and lobe-finned fish, in addition to teleosts. This shows that nIL-1F is not exclusively present in teleosts as previously suggested. Our analyses of exon-intron structures, intron phases, phylogeny and synteny clearly show the separation of IL-1β into groups; type I and type II, which likely is a result of the third whole genome duplication (3R WGD). The phylogenetic analysis shows that most teleosts have both type I and type II. Furthermore, we have determined transcription levels of the IL-1 ligands in leukocytes and 16 different tissues, and their responses upon in vitro stimulation with seven different ligands. In addition, we have identified the IL-1 receptors IL-1R1, IL-1R2, IL-1R4 (ST2/IL-33 receptor/IL-1RL), IL-1R5 (IL-18R1), and partial sequences of DIGIRR and IL-1R3 (IL-RAcP). Identification of immune molecules and description of innate responses in lumpfish is interesting for comparative and evolutionary studies and our study constitutes a solid basis for further functional analyses of IL-1 ligands and receptors in lumpfish. Furthermore, since lumpfish are now farmed in large numbers to be used as cleaner fish for removal of sea lice on farmed salmon, in-depth knowledge of key immune molecules, signaling pathways and innate immune responses is needed, as the basis for design of efficient immune prophylactic measures such as vaccination.

show abstract

The proinflammatory cytokines TNF-α and IL-6 in lumpfish (Cyclopterus lumpus L.) -identification, molecular characterization, phylogeny and gene expression analyses

Cited by 24 publications

References 59 publications

Functional characterization of TNF‐α in pufferfish (Takifugu obscurus) in immune response and apoptosis against Aeromonas hydrophila

Functional characterization of TNF‐α in pufferfish (Takifugu obscurus) in immune response and apoptosis against Aeromonas hydrophila

FKN/NR Signaling Pathway Regulates Hippocampal Inflammatory Responses: the Survival of Hippocampal Neurons in Diabetic Rats with Chronic Unpredictable Mild Stress

Interleukin-1 Ligands and Receptors in Lumpfish (Cyclopterus lumpus L.): Molecular Characterization, Phylogeny, Gene Expression, and Transcriptome Analyses

Contact Info

Product

Resources

About