The Promoter Activity of the Phospholipase C-γ2 Gene Is Regulated by a Cell-Type-Specific Control Element

Lee, Seung-Jae; Bahk, Young Yil; Yun, Doo Hee; Lee, He-Jin; Lee, Young Han; Ryu, Sung Ho; Suh, Pann‐Ghill

doi:10.1089/dna.1997.16.485

Cited by 4 publications

(4 citation statements)

References 40 publications

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“…So far, the promoter regions of PLCγ1, γ2, and β3 have been isolated [19–22], and it has been reported that these regions are GC‐rich and have no TATA box. We also noticed that the promoter region of PLCδ4 is GC‐rich and has no TATA box, indicating that these properties are common among the PLC isozymes.…”

Section: Resultsmentioning

confidence: 99%

Growth factor‐induced promoter activation of murine phospholipase C δ4 gene

Fukami

Takenaka

Nagano

et al. 2000

European Journal of Biochemistry

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Phospholipase C d4 (PLCd4) is one of the delta-type PLC isozymes, the expression of which is induced in nuclei by treatment with serum and also in some cancer cells. We isolated and analyzed a promoter region of the murine PLCd4 gene. DNA sequence analysis showed that this region is GC-rich and has no TATA box, and the region from 2143 to 2127 was found, by luciferase activity and gel mobility-shift assay, to be essential for transcription of PLCd4. We also found that the promoter activity of PLCd4 was stimulated by treatment with growth factors such as bradykinin, lysophosphatidic acid, and Ca 21 ionophore in addition to serum. In parallel, we detected PLCd4 mRNA induction and an increase in complex formation of the promoter region and nuclear protein from HeLa cells on stimulation with these growth factors. Finally, we found that trapping the growth factor-induced cytoplasmic Ca 21 -inhibited activation of the promoter activity and protein induction in nuclei. These results show that PLCd4 may have an important role in nuclei in response to growth factors, and its expression may be partially regulated by an increase in cytoplasmic Ca 21.

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Section: Resultsmentioning

confidence: 99%

Growth factor‐induced promoter activation of murine phospholipase C δ4 gene

Fukami

Takenaka

Nagano

et al. 2000

European Journal of Biochemistry

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“…Daudi (human Burkitt lymphoma) cells (1-2 × 10 7 ) were electroporated with 20 µg of plasmid DNA as described (Lee et al, 1997). After 48 hrs of incubation, the cells were harvested and soluble, cytosolic extract was prepared by three cycles of freezing-thawing.…”

Section: Cell Culture Transient Transfection and Cat Assaysmentioning

confidence: 99%

“…After 48 hrs of incubation, the cells were harvested and soluble, cytosolic extract was prepared by three cycles of freezing-thawing. The CAT activities were assayed with 100-150 µg of the extracts as described previously (Lee et al, 1997).…”

Section: Cell Culture Transient Transfection and Cat Assaysmentioning

confidence: 99%

“…The mixture was heated for 10 min at 75 o C, incubated at 37 o C overnight, then ethanol-precipitated. cDNA was prepared from the hybrids as described elsewhere (Lee et al, 1997). Extension was performed at 40 o C for 75 min and the reaction was stopped by addition of 1 ml of 0.5 M EDTA and 1 ml of DNase-free RNase (Boeringer Mannheim, Mannheim, Germany), incubated for 30 min at 37 o C, and extracted with phenolchloroform.…”

Section: Primer Extension Analysismentioning

confidence: 99%

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Cloning and characterization of 5'-upstream region of human phospholipase C-β2 gene

Yun

Lee²,

Kim³

et al. 2001

Exp Mol Med

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5'-upstream region of the phospholipase C-β2 gene, 810 bp, was cloned and characterized. S1 nuclease mapping and primer extension analyses revealed that a single transcriptional start site locates at 284 nucleotides upstream from the beginning of translation. The 5'-upstream region lacks both TATA motif and typical initiator sequence, but retains GC-rich segment. Two putative regulatory regions, a negative region (−636/−588) and a positive region (−98/ −13) were identified in the upstream region of PLC-β2 gene. We suggest that the transcription of PLC-β2 may be regulated by binding of regulatory proteins to the negative and/or positive regulatory regions located in the upstream of the gene.

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Independent Repression of a GC-rich Housekeeping Gene by Sp1 and MAZ Involves the Same cis-Elements

Song

Ugai²,

Kanazawa

et al. 2001

Journal of Biological Chemistry

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The transcription factors Sp1 and MAZ (Myc-associated zinc finger protein) contain several zinc finger motifs, and each functions as both a positive and a negative regulator of gene expression. In this study, we characterized the extremely GC-rich promoter of the human gene for MAZ, which is known as a housekeeping gene. Unique symmetrical motifs in the promoter region (nucleotides ؊383 to ؊334) were essential for the expression of the gene for MAZ, whereas an upstream silencer element (nucleotides ؊784 to ؊612) was found to act in a position-dependent but orientation-independent manner. Sp1 and MAZ bound to the same cis-elements in the GC-rich promoter, apparently sharing DNA-binding sites. The relative extent of binding of Sp1 and MAZ to these cis-elements corresponded to the extent of negative regulation of the expression of the gene for MAZ in various lines of cells. Furthermore, novel repressive domains in both Sp1 (amino acids 622-788) and MAZ (amino acids 127-292) were identified. Suppression by Sp1 and suppression by MAZ were independent phenomena; histone deacetylases were involved in the autorepression by MAZ itself, whereas DNA methyltransferase 1 was associated with suppression by Sp1. Our results indicate that both deacetylation and methylation might be involved in the regulation of expression of a single gene via the actions of different zinc finger proteins that bind to the same cis-elements.

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The Promoter Activity of the Phospholipase C-γ2 Gene Is Regulated by a Cell-Type-Specific Control Element

Cited by 4 publications

References 40 publications

Growth factor‐induced promoter activation of murine phospholipase C δ4 gene

Growth factor‐induced promoter activation of murine phospholipase C δ4 gene

Cloning and characterization of 5'-upstream region of human phospholipase C-β2 gene

Independent Repression of a GC-rich Housekeeping Gene by Sp1 and MAZ Involves the Same cis-Elements

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