The Promoting Effects of Geniposidic Acid and Aucubin in Eucommia ulmoides OLIVER Leaves on Collagen Synthesis.

Abstract

We have reported that collagen synthesis was stimulated by the administration of a hot water extract from the leaves of Eucommia ulmoides OLIVER, Eucommiaceae (Du-Zhong leaves) in false aged model rats. In this paper, we set out to examine the compounds in Du-Zhong leaves that stimulated collagen synthesis in false aged model rats. In experiment 1, a methanol extract of Du-Zhong leaves also stimulated collagen synthesis in aged model rats. An acetone fraction was derived from the methanol extract by silica gel chromatography in experiment 2. The acetone fraction mainly contained iridoides mono-glycosides such as geniposidic acid and aucubin. The administration of geniposidic acid or aucubin stimulated collagen synthesis in aged model rats in experiments 3 and 4 (significance (p<0.05)). The reported pharmacological effects of Du-Zhong leaves, including healing organs and strengthening bone and muscle, are closely related to collagen metabolism. It appears that geniposidic acid and aucubin are the actual compounds in Du-Zhong which caused the effect in our experiments.

Eucommia ulmoides Oliv. antagonizes H2O2-induced rat osteoblastic MC3T3-E1 apoptosis by inhibiting expressions of caspases 3, 6, 7, and 9

“…Our previous study also indicated that the compound of calcium phosphate cement and EuO extracts promoted proliferation and adhesion of MC3T3-E1 osteoblasts in vitro (Lin et al, 2008). The pharmacological effects of EuO leaves on bone and muscle are closely related to collagen metabolism (Li et al, 1998). Granuloma formation and collagen synthesis were significantly increased by the administration of methanol extracts of EuO cortex (Li et al, 2000a).…”

“…Li et al (1998) reported that collagen synthesis in the aged-rat model was stimulated by the administration of hot water-extracts from EuO leaves. Ha et al (2003) reported that EuO influences osteoblastic differentiation and prevents bone loss, and concluded that an EuO fraction with high polarity might contain active constituents stimulating the proliferation of osteoblasts.…”

Aucubin prevents interleukin-1 beta induced inflammation and cartilage matrix degradation via inhibition of NF-κB signaling pathway in rat articular chondrocytes

“…Aucubin, a natural compound has been found in various plants including leaves of Aucuba japonica [15] and Eucommia ulmoides [16], proves to possess a variety of pharmacological effects [17][18][19]; [20], notably, anti-inflammatory effect [21]. It has been reported to have blocked the translocation of NF-κB by inhibition of IκB degradation and phosphorylation, a critical step in early inflammatory responses [22].…”

Comparison of techniques for the extraction of the hypotensive drugs geniposidic acid and geniposide from Eucommia ulmoides

“…1b). GPA promotes collagen synthesis [11], and GPS has anti-inflammatory activity [12], activity against the *Corresponding author. E-mail: xqchen@mail.csu.edu.cn tumor-promoting compound 12-O-tetradecanoyl-phorbol-13-acetate [13][14], and is a treatment for liver disorder [15].…”