The Protective Effect of Basic Fibroblast Growth Factor on Diabetic Nephropathy Through Remodeling Metabolic Phenotype and Suppressing Oxidative Stress in Mice

Wei, Tingting; Shu, Qi; Ning, Jie; Wang, Shuaijie; Li, Chen; Zhao, Liangcai; Zheng, Hong; Gao, Hongchang

doi:10.3389/fphar.2020.00066

Cited by 18 publications

(12 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Apoptosis can promote tubular cell hypertrophy, glomerular basement membrane dilatation, and accumulation of extracellular matrix, which contribute to progressive tubulointerstitial fibrosis and glomerulosclerosi. Therefore, it is considered to be an important inducer of renal remodeling 8 . Furthermore, autophagy is negatively correlated with renal fibrosis including glomerular extracellular matrix accumulation and tubulointerstitial fibrosis 3 , 9 , 10 , while autophagy deficiency promotes the progression of glomerular lesions and tubulointerstitial damage 3 .…”

Section: Introductionmentioning

confidence: 99%

CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy

Zheng

Tan

Shang

et al. 2021

J of Diabetes Invest

View full text Add to dashboard Cite

Objective The role of cell death‐inducing DFF45‐like effector C (CIDEC) in insulin resistance has been established, and it is considered to be an important trigger factor for the progression of diabetic nephropathy (DN). We intend to explore whether CIDEC plays an important role in the regulation of DN and its potential mechanism. Methods High‐fat diet and low dose streptozotocin were used to establish type 2 diabetic rat model. We investigate the role of CIDEC in the pathogenesis and process of DN through histopathological analysis, western blot and gene silencing. Meanwhile, the effect of CIDEC on renal tubular epithelial cells stimulated by high glucose was also verified. Results DM group exhibited glucose and lipid metabolic disturbance, with hypertrophy of kidneys, damaged renal function, increased apoptosis, decreased autophagy, glomerulosclerosis and interstitial fibrosis. CIDEC gene silencing improved metabolic disorder and insulin resistance, alleviated renal hypertrophy and renal function damage, decreased glomerular and tubular apoptosis, increased autophagy and inhibited renal fibrosis. At the cellular level, high glucose stimulation increased CIDEC expression in renal tubular epithelial cells, accompanied by increased apoptosis and decreased autophagy. CIDEC gene silencing can improve autophagy and reduce apoptosis. At the molecular level, CIDEC gene silencing also decreased the expression of early growth response factor (EGR)1 and increased the expression of adipose triglyceride lipase (ATGL). Conclusion CIDEC gene silencing may delay the progression of DN by restoring autophagy activity and inhibiting apoptosis with the participation of EGR1and ATGL.

show abstract

Section: Introductionmentioning

confidence: 99%

CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy

Zheng

Tan

Shang

et al. 2021

J of Diabetes Invest

View full text Add to dashboard Cite

show abstract

“…Mice with fibroblast-specific disruption of the FGFR2 gene demonstrated decreased total collagen deposition, fibronectin, and alpha smooth muscle actin expression in an ischemia-reperfusion model [56,57]. On the other hand, treatment with bFGF alleviated oxidative stress and apoptosis in the kidney in db/db mice [58]. In a model of streptozotocin-induced diabetes in Sprague-Dawley rats, bFGF delayed the progression of diabetic nephropathy, possibly by inhibiting NF-κB and silencing of TGF-β1, MCP-1, IL-6, and IL-1β [59].…”

Section: Discussionmentioning

confidence: 99%

Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-Albuminuric Diabetic Kidney Disease

Климонтов

Korbut

Орлов

et al. 2020

JCM

View full text Add to dashboard Cite

A panel of cytokines and growth factors, mediating low-grade inflammation and fibrosis, was assessed in patients with type 2 diabetes (T2D) and different patterns of chronic kidney disease (CKD). Patients with long-term T2D (N = 130) were classified into four groups: no signs of CKD; estimated glomerular filtration rate (eGFR) <60 mL/min/1.73 m2 without albuminuria; albuminuria and eGFR ≥60 mL/min/1.73 m2; albuminuria and eGFR <60 mL/min/1.73 m2. Thirty healthy subjects were acted as control. Twenty-seven cytokines and growth factors were assessed in serum by multiplex bead array assay. Serum hs-CRP, urinary nephrin, podocine, and WFDC2 were measured by ELISA. Patients with T2D showed elevated IL-1Ra, IL-6, IL-17A, G-CSF, IP-10, MIP-1α, and bFGF levels; concentrations of IL-4, IL-12, IL-15, INF-γ, and VEGF were decreased. IL-6, IL-17A, G-CSF, MIP-1α, and bFGF correlated negatively with eGFR; IL-10 and VEGF demonstrated negative associations with WFDC2; no relationships with podocyte markers were found. Adjusted IL-17A and MIP-1α were predictors of non-albuminuric CKD, IL-13 predicted albuminuria with preserved renal function, meanwhile, IL-6 and hsCRP were predictors of albuminuria with eGFR decline. Therefore, albuminuric and non-albuminuric CKD in T2D patients are associated with different pro-inflammatory shifts in the panel of circulating cytokines.

show abstract

“…The results are consistent with various studies that reported the factors secreted from MSC contain antioxidants as one of the predominant elements, which are included in CM and exert anti-oxidative effects in paracrine mechanisms [ 13 , 58 , 59 , 60 ]. In particular, numerous growth factors found in CM of human amnion tissue and AMSC [ 61 ] have been identified to function as antioxidants including insulin-like growth factor [ 62 ], platelet-derived growth factor [ 63 ], epidermal growth factor [ 64 ], hepatocyte growth factor [ 65 ] and fibroblast growth factor [ 66 ]. Moreover, pre- and post-IVC medium analysis revealed that the activities of SOD and catalase were higher in culture medium supplemented with AMSC-CM than the medium with ASC-CM ( Figure 5 and Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Anti-Oxidative Effect of Human Adipose- and Amniotic Membrane-Derived Mesenchymal Stem Cell Conditioned Medium on Mouse Preimplantation Embryo Development

Kim

et al. 2021

Antioxidants

View full text Add to dashboard Cite

Oxidative stress is a major cause of damage to the quantity and quality of embryos produced in vitro. Antioxidants are usually supplemented to protect embryos from the suboptimal in vitro culture (IVC) environment. Amniotic membrane-derived mesenchymal stem cells (AMSC) have emerged as a promising regenerative therapy, and their paracrine factors with anti-oxidative effects are present in AMSC conditioned medium (CM). We examined the anti-oxidative potential of human AMSC-CM treatment during IVC on mouse preimplantation embryo development and antioxidant gene expression in the forkhead box O (FoxO) pathway. AMSC-CM (10%) was optimal for overall preimplantation embryo developmental processes and upregulated the expression of FoxOs and their downstream antioxidants in blastocysts (BL). Subsequently, compared to adipose-derived mesenchymal stem cell (ASC)-CM, AMSC-CM enhanced antioxidant gene expression and intracellular GSH levels in the BL. Total antioxidant capacity and SOD activity were greater in AMSC-CM than in ASC-CM. Furthermore, SOD and catalase were more active in culture medium supplemented with AMSC-CM than in ASC-CM. Lastly, the anti-apoptotic effect of AMSC-CM was observed with the regulation of apoptosis-related genes and mitochondrial membrane potential in BL. In conclusion, the present study established AMSC-CM treatment at an optimal concentration as a novel antioxidant intervention for assisted reproduction.

show abstract

The Protective Effect of Basic Fibroblast Growth Factor on Diabetic Nephropathy Through Remodeling Metabolic Phenotype and Suppressing Oxidative Stress in Mice

Cited by 18 publications

References 48 publications

CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy

CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy

Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-Albuminuric Diabetic Kidney Disease

Comparison of Anti-Oxidative Effect of Human Adipose- and Amniotic Membrane-Derived Mesenchymal Stem Cell Conditioned Medium on Mouse Preimplantation Embryo Development

Contact Info

Product

Resources

About