The protective effect of erythropoietin infusion on testicular torsion/detorsion: an experimental study

Köseoğlu, Burhan; Yılmaz, Engin; Ceylan, Kadir; Uzun, Ersan; Bayram, İrfan; Hızlı, Fatih

doi:10.1007/s11255-008-9418-8

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…A dose of 0.7 mg/kg was injected intraperitoneally in the rats of group E (sildenafil group). These doses of erythropoietin and sildenafil have been reported by previous studies (16–22). …”

Section: Methodssupporting

confidence: 84%

“…The role of erythropoietin and sildenafil against ischemia/reperfusion injury during testicular torsion and detorsion has been evaluated in rats in several studies (16,21,31,32). The intraperitoneal injection of erythropoietin prior to detorsion decreases the histological damage of the testis by reducing the damage and the apoptotic rate of germinal cells, reducing the necrotic lesions of seminiferous tubules and preserving their morphology (16,21,31,32).…”

Section: Discussionmentioning

confidence: 99%

“…The intraperitoneal injection of erythropoietin prior to detorsion decreases the histological damage of the testis by reducing the damage and the apoptotic rate of germinal cells, reducing the necrotic lesions of seminiferous tubules and preserving their morphology (16,21,31,32). Erythropoietin also retains its protective role when it is administered soon after detorsion (21,22).…”

Section: Discussionmentioning

confidence: 99%

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Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats

Kostakis

Zavras

Damaskos

et al. 2017

Experimental and Therapeutic Medicine

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Testicular torsion/detorsion causes severe tissue damage due to ischemia/reperfusion injury. The present study investigated the protective effect of erythropoietin and sildenafil against ischemia/reperfusion injury following unilateral testicular torsion/detorsion in adult rats. A total of 28 adult male rats were included, and were divided into the following groups: Group A (n=5), sham operated; groups B (n=5), C (n=5), D (n=5) and E (n=8), undergoing right testis torsion and detorsion after 90 min. Group B received no drug treatment. Rats in the groups C and D received low-dose (1,000 IU/kg) or high-dose (3,000 IU/kg) erythropoietin, while those in group E received sildenafil (0.7 mg/kg), through intraperitoneal injection after 60 min of torsion. The right testis was extracted 24 h after detorsion, and the tissue was subjected to histopathological examination and immunohistochemical assessment of cleaved caspase-3 expression. Histological alterations and the quality of spermatogenesis were scored according to the Cosentino and the Johnsen scoring systems, respectively. The results demonstrated normal testicular architecture in group A, while the other groups showed ischemic cellular damages, with the worst scores observed in group B. Groups D and E presented better scores compared with group C. Regarding the quality of spermatogenesis, the best scores were observed in group A, and the worst in group B. Groups C, D and E presented similar results, which were improved in comparison to group B, however, not compared to group A. Furthermore, cleaved caspase-3 levels were lower in groups A, D and E, with equal results observed. Group C had higher levels of cleaved caspase-3 compared with these groups, but lower than group B, which presented the highest cleaved caspase-3 levels. In conclusion, erythropoietin and sildenafil protect testis from ischemia/reperfusion injury by decreasing cellular damage and attenuating apoptosis.

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Section: Methodssupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats

Kostakis

Zavras

Damaskos

et al. 2017

Experimental and Therapeutic Medicine

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“…It was found that erythropoietin can decrease cell damage and apoptosis. Köseoğlu et al concludes that erythropoietin preserves the intact somniferous tubular morphology, lowers the percentage of necrotic seminiferous tubules, and reduces the histological damage [13]. One study used mouse model to assess effect of erythropoietin, but as darbepoetin α , on ischemia caused by testis torsion finding that it affects histological grading.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Erythropoietin on Ischemia/Reperfusion Injury after Testicular Torsion/Detorsion: A Randomized Experimental Study

et al. 2013

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This study was conducted to investigate the protective effect of erythropoietin (EPO) on ischemia/reperfusion related changes after testicular torsion/detorsion. In a randomized experimental trial 30 male rats were randomly allocated into six equal groups of five rats each. Group I (orchiectomy for histopathologic examination), group II (sham operation), group III (torsion for 2 hours, and ischemia/detorsion for 24 hours, and orchiectomy); group IV (torsion for 2 hours, ischemia/detorsion for 24 hours with erythropoietin injection then orchiectomy), group V (torsion for 2 hours and detorsion and EPO injection and orchiectomy 1 week later, group VI (torsion for 2 hours/detorsion and orchiectomy 1 week later). Two groups (groups 4 and 5) received different protocols of erythropoietin administration after testicular torsion/distortion. other groups were not receiving erythropoietin. Johnsen's spermatogenesis scoring method and Cosentino's histologic staging method were used to assess main outcome measures of the study. After the experimentation, Johnsen's score in EPO Groups was statistically different from the score in some groups not receiving erythropoietin. Cosentino's score in EPO groups was statistically different from the score in all groups not receiving erythropoietin. Neovascularization, vascular necrosis, vascular congestion, edema, hemorrhage, and acute inflammation were observed in some groups. This study shows short-term protective efficacy of erythropoietin on rat testicular injury after ischemia/reperfusion.

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“…Kö seog lu et al (2009) demonstrated that rHuEPO administration significantly influenced the rescue of testicular function by preserving the intact seminiferous tubular morphology, lowering the percentage of necrotic seminiferous tubules and significantly reducing histological damage after induced testicular torsion/detorsion in male rats. In the current study, no histological damage was observed in the rabbit testis upon completion of the experiment, suggesting that EPO gene transfer or rHuEPO administration did not damage the rabbit testis.…”

Section: Discussionmentioning

confidence: 97%

Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm

Collares

Campos

Urtiaga

et al. 2013

Animal

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Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to evaluate the toxicological effects of non-viral (EPO) gene transfer on sperm motility, viability, morphology and concentration. Rabbit EPO cDNA was cloned into a pTarget mammalian expression vector. Rabbits were administered with: (1) pTarget/EPO vector, (2) recombinant human EPO (rHuEpo) and (3) saline (control). Both pTarget/EPO and rHuEpo significantly increased (P , 0.05) hematocrit levels 1 week after injection and they remained significantly higher than the control for up to 5 weeks (P , 0.05), showing that both EPO treatments were effective in stimulating the production of red blood cells in rabbits. The EPO gene transfer or rHuEPO administration had no significant effect (P . 0.05) on sperm motility, vigor, viability, concentration or morphology in the testis.

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The protective effect of erythropoietin infusion on testicular torsion/detorsion: an experimental study

Abstract: Administration of EPO significantly influenced the rescue of testicular function by preserving the intact seminiferous tubular morphology, lowering the percentage of necrotic seminipherous tubules, and significantly reducing histological damage (P < 0.05).

Cited by 7 publications

References 17 publications

Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats

Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats

The Effect of Erythropoietin on Ischemia/Reperfusion Injury after Testicular Torsion/Detorsion: A Randomized Experimental Study

Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm

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