The Protective Effect of Eupatilin against Hydrogen Peroxide-Induced Injury Involving 5-Lipoxygenase in Feline Esophageal Epithelial Cells

Abstract

In this study, we focused to identify whether eupatilin (5,7-dihydroxy-3',4',6-trimethoxyflavone), an extract from Artemisia argyi folium, prevents H2O2-induced injury of cultured feline esophageal epithelial cells. Cell viability was measured by the conventional MTT reduction assay. Western blot analysis was performed to investigate the expression of 5-lipoxygenase by H2O2 treatment in the absence and presence of inhibitors. When cells were exposed to 600 µM H2O2 for 24 hours, cell viability was decreased to 40%. However, when cells were pretreated with 25~150 µM eupatilin for 12 hours, viability was significantly restored in a concentration-dependent manner. H2O2-treated cells were shown to express 5-lipoxygenase, whereas the cells pretreated with eupatilin exhibited reduction in the expression of 5-lipoxygenase. The H2O2-induced increase of 5-lipoxygenase expression was prevented by SB202190, SP600125, or NAC. We further demonstrated that the level of leukotriene B4 (LTB4) was also reduced by eupatilin, SB202190, SP600125, NAC, or nordihydroguaiaretic acid (a lipoxygenase inhibitor) pretreatment. H2O2 induced the activation of p38MAPK and JNK, this activation was inhibited by eupatilin. These results indicate that eupatilin may reduce H2O2-induced cytotoxicity, and 5-lipoxygenase expression and LTB4 production by controlling the p38 MAPK and JNK signaling pathways through antioxidative action in feline esophageal epithelial cells.

Eupatilin inhibits EGF-induced JB6 cell transformation by targeting PI3K

“…eupatilin, a dietary flavone compound, has anti-ulcer, anti-inflammatory and cell cycle regulator inhibitory effects (6,8,36). Accumulating evidence shows that eupatilin has antitumor function against the different types of cancer, including gastric and endometrial cancer (36). In the present study, we found that eupatilin could inhibit JB6 cell proliferation and growth in a dose-dependent manner (Fig.…”

“…Flavonoids are well known as promising chemopreventive agents against human cancers. eupatilin, a dietary flavone compound, has anti-ulcer, anti-inflammatory and cell cycle regulator inhibitory effects (6,8,36). Accumulating evidence shows that eupatilin has antitumor function against the different types of cancer, including gastric and endometrial cancer (36).…”

Cytotoxicity and Structure-activity Relationships of Naphthyridine Derivatives in Human Cervical Cancer, Leukemia, and Prostate Cancer

“…Cytotoxicities were measured with the MTT-based colorimetric assay as described in the Table 1 legend [28-31]. …”

Eupatilin attenuates diabetic nephropathy by upregulating matrix metalloproteinase-9 expression in diabetic rat kidney

“…Eupatilin is a flavonoid isolated from Artemisia princeps Pampanini (Asteraceae) [10]. It possesses strong antioxidant and antiinflammatory properties [11,12]. Moreover, it has been reported that eupatilin exerts gastroprotective effects by attenuating gastric mucosal injuries [13].…”