The protective effect of Satureja bachtiarica hydroalcoholic extract on streptozotocin‐induced diabetes through modulating glucose transporter 2 and 4 expression and inhibiting oxidative stress

Abstract

Context: Oxidative stress plays an important role in development of diabetes mellitus. Satureja bachtiarica Bunge (Lamiaceae) is a rich source of bioactive compounds with antioxidant properties. Objective: This study investigates the antidiabetic effect of hydroalcoholic extract of aerial parts of S. bachtiarica . Methods and materials: Male Wistar rats were randomly divided into six groups ( n = 8) including control (normal saline), diabetic [Streptozotocin (STZ)], intervention (STZ plus hydroalcoholic extract of S. bachtiarica at doses of 75, 150 and 250 mg/kg/d) and positive control (STZ plus captopril 50 mg/kg/d) groups. A single intraperitoneal (IP) injection of STZ (60 mg/kg) was used to induce diabetes and IP therapy with drugs was performed for four weeks. Results : In diabetic rats, serum total antioxidant capacity (TAC) decreased significantly, but glucose, alkaline phosphatase ( ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (GGT) and malondialdehyde (MDA) increased significantly as compared to the control ( p < 0.05). Treatment with extract (250 mg/kg) caused a significant decline in serum glucose, GGT, ALT, AST and MDA as well as a significant increase in serum TAC ( p < 0.05). During the intervention period, diabetic rats showed significant weight loss, but extract (250 mg/kg) treated rats did not show any weight loss. Extract (250 mg/kg) up-regulated GLUT2 expression and down-regulated GLUT4 expression in the liver ( p < 0.05). S . bachtiarica extract at all dosage levels prevented STZ-induced histological damage of liver, kidney and pancreas. Discussion and conclusions: S . bachtiarica extract exhibits antidiabetic effects through modulation of oxidative stress and expression of GLUT2 and GLUT4 .

Investigation of the protective and therapeutic effects of β-aminoisobutyric acid (BAIBA) and Thymoquinone in the diabetic nephropathy

“…1). STZ is an antibiotic produced by Streptomyces achromogenes, causing irreversible damage with a direct toxic effect on pancreatic βcells and used to create experimental DM (Salih et al, 2014;Dhanavathy, 2015;Furman, 2015;Bathina et al, 2017;Joudaki & Setorki, 2019). Diabetic nephropathy is the main reason of morbidity and death in diabetic people.…”

“…This information explains the increase in relative kidney weight in the STZ group in the current study. The probable cause of body weight loss in STZ group rats is that because the glucose in the blood cannot be used by the body cells as a source of metabolic energy, the cells use amino acids formed by the breakdown of fats and structural proteins as energy sources (Giribabu et al, 2017;Joudaki & Setorki, 2019). It has been proven that TQ lowers blood sugar and is beneficial in the treatment of DM by increasing insulin secretion and sensitivity, increasing glucose utilization and also decreasing hepatic glucose production.…”

“…Chronic hyperglycemia; It increases the production of reactive oxygen species (ROS) and triggers oxidative stress, causing damage to small blood vessels, causing tissue and organ failure. ROS such as hydroxyl and superoxide radicals attack cell cellular macromolecules such as carbohydrates, nucleic acids, lipids and proteins, causing cell damage or death (Xu et al, 2005;Tolman et al, 2007;Ros et al, 2011;Karandrea et al, 2017;Joudaki & Setorki, 2019). In addition, pro inflammation due to hyperglycemia and oxidative stress caused an increase in TNF-α, a proinflammatory cytokine (Cayakar, 2018).…”

“…In a study conducted by Wang et al (2017) reported that BAIBA treatment can improve pathological changes in the kidneys and that BAIBA has the potential to be used as a medicine in the treatment of renal fibrosis diseases (Kanter, 2009;Al-Trad et al, 2016;Shaterzadeh-Yazdi et al, 2018). STZ is an antibiotic produced by Streptomyces achromogenes, cause the destruction of β cells in the pancreas and used to create experimental DM (Furman 2015;Bathina et al, 2017;Joudaki & Setorki, 2019). The mechanism of the action of STZ on pancreatic β-cells has not been completely understood yet; however, it is believed that oxidative stress plays an important role in the development of complications from STZ (Sadek et al, 2017).…”

The ameliorative activity of Chrysobalanus orbicularis in streptozotocin-induced type II diabetes mellitus rat model

“…Hindrance of insulin signalling in skeletal muscle, liver and fat tissues because of the advanced insulin resistance is the primary driver of type II diabetes mellitus. This disorder advances and prompts organ dysfunction and unexpected death (Joudaki and Setorki, 2019). Exogenous variables like ecological factors just as hereditary qualities, diet and weight contribute extraordinarily to the improvement of this ailment (Piero et al, 2015).…”