The protective effect of nitronyl nitroxide radical on peroxidation of A549 cell damaged by iron overload

He, Shao-Min; Lei, Yuhua; Wang, Jianmin; Geng, Lin; Wang, Shuping; Zhao, Juan; Hou, Yi-Fang

doi:10.1016/j.msec.2019.110189

Cited by 11 publications

(5 citation statements)

References 32 publications

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“…The increased expression of TSPAN5 may be related to the activation of the NOTCH signaling pathway by increased cellular uptake of iron, while the downregulation of MSMO1 expression may be mechanistically related to heme metabolism, but relevant studies are lacking [56][57][58][59]. In addition, intracellular iron overload usually leads to downregulation of BCL2 and induces apoptosis; interestingly, the expression of BCL2 was upregulated in the IPAH lung samples we analyzed, which may result in abnormal antiapoptotic phenotypic changes in pulmonary vascular endothelial cells and pulmonary vascular smooth muscle cells due to factors other than iron metabolism [60][61][62]. Five of the key genes (BCL2, MSMO1, SLC7A11, TSPAN5, and TXNRD1) as target genes may be regulated by 6 DRmiRNAs (miR-483-5p, miR-27a-3p, miR-27b-3p, miR-26b-5p, miR-199a-5p, and miR-23b-3p), which could be a potential crosstalk between iron metabolism and miRNA regulation in IPAH.…”

Section: Discussionmentioning

confidence: 97%

Iron Metabolism and Idiopathic Pulmonary Arterial Hypertension: New Insights from Bioinformatic Analysis

Zou

Qiu

Lai

et al. 2021

BioMed Research International

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Idiopathic pulmonary arterial hypertension (IPAH) is a rare vascular disease with a poor prognosis, and the mechanism of its development remains unclear. Further molecular pathology studies may contribute to a comprehensive understanding of IPAH and provide new insights into diagnostic markers and potential therapeutic targets. Iron deficiency has been reported in 43-63% of patients with IPAH and is associated with reduced exercise capacity and higher mortality, suggesting that dysregulated iron metabolism may play an unrecognized role in influencing the development of IPAH. In this study, we explored the regulatory mechanisms of iron metabolism in IPAH by bioinformatic analysis. The molecular function of iron metabolism-related genes (IMRGs) is mainly enriched in active transmembrane transporter activity, and they mainly affect the biological process of response to oxidative stress. Ferroptosis and fluid shear stress and atherosclerosis pathways may be the critical pathways regulating iron metabolism in IPAH. We further identified 7 key genes (BCL2, GCLM, MSMO1, SLC7A11, SRXN1, TSPAN5, and TXNRD1) and 5 of the key genes (BCL2, MSMO1, SLC7A11, TSPAN5, and TXNRD1) as target genes may be regulated by 6 dysregulated miRNAs (miR-483-5p, miR-27a-3p, miR-27b-3p, miR-26b-5p, miR-199a-5p, and miR-23b-3p) in IPAH. In addition, we predicted potential IPAH drugs—celastrol and cinnamaldehyde—that target iron metabolism based on our results. These results provide insights for further definition of the role of dysregulated iron metabolism in IPAH and contribute to a deeper understanding of the molecular mechanisms and potential therapeutic targets of IPAH.

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Section: Discussionmentioning

confidence: 97%

Iron Metabolism and Idiopathic Pulmonary Arterial Hypertension: New Insights from Bioinformatic Analysis

Zou

Qiu

Lai

et al. 2021

BioMed Research International

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“…To further confirm the initiation of oxidative stress in PD devel- are widely used determine the redox state in brain. 51,52 This study found that ROT and LPS synergistically increased MDA level, especially first stimulation of ROT followed by LPS-induced apparent increase in MDA level, while SOD level detection were opposite. In addition, as a major regulator of oxidative stress, Nrf2 could regulate the redox state of cells in response to oxidative stress.…”

Section: Discussionmentioning

confidence: 71%

“…To further confirm the initiation of oxidative stress in PD development, the effects of LPS and ROT sequential administration on brain oxidation condition and Nrf2 signaling were assessed. MDA, one of the most commonly used indicators of lipid peroxidation, and SOD, which could counter the destructive reaction of superoxide, are widely used determine the redox state in brain 51,52 . This study found that ROT and LPS synergistically increased MDA level, especially first stimulation of ROT followed by LPS‐induced apparent increase in MDA level, while SOD level detection were opposite.…”

Section: Discussionmentioning

confidence: 79%

Synergistic effects of lipopolysaccharide and rotenone on dopamine neuronal damage in rats

Qian

et al. 2023

CNS Neurosci Ther

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Parkinson's disease (PD) is one of the most common degenerative diseases in the central nervous system (CNS), with high prevalence rate and even a tendency to low age. 1 PD is major characterized by progressive loss of dopaminergic (DA) neurons in the substantia nigra (SN) of midbrain. With the development of PD, it is manifested as rest tremor, rigidity, and dyskinesia, which severely affects the normal life of patients. 2 However, the pathogenesis of PD is still unclear. Until now, oxidative stress and neuroinflammation are considered to be important inducing factors.Oxidative stress, known as a potential cause of cell dysfunction and eventual death, plays a non-negligible role in complex neurodegenerative cascades. Normally, cells in the body are dependent on a series of balanced redox reactions that produce energy and take nutrients to synthesize the necessary cellular components to support

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“…These results revealed that the ESR spin probe technique could be used to study samples using non-TEMPO derivatives to study the chemical and biological structures of nanosized materials (such as nanosized cavities, polymers, membranes, and dental materials). The existence of the nanosized spaces containing the imino or nitronyl nitroxide groups has been reported in recent years. ,, The results reported herein can be used to develop such novel systems. The radicals used may be introduced inside the preformed pores in the polymer samples by exposing in a sealed test tube to conditions of reduced pressure or penetrating in the radical solution.…”

Section: Discussionmentioning

confidence: 99%

“…The existence of the nanosized spaces containing the imino or nitronyl nitroxide groups has been reported in recent years. 34,65,66 The results reported herein can be used to develop such novel systems. The radicals used may be introduced inside the preformed pores in the polymer samples by exposing a sealed test tube to conditions of reduced pressure or penetrating in the radical solution.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Various Organic Radicals Dispersed in Polymethylmethacrylate Matrices Using the Electron Spin Resonance Spectroscopy Technique

et al. 2021

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The electron spin resonance (ESR) spectroscopy technique was used to study various organic radicals, such as 2,2,6,6-tetramethyl-1-piperidinyloxyl (TEMPO), 4-hydroxy-TEMPO (TEMPOL), 2-X-nitronylnitroxide (2-X-NN, X = Ph, NO 2 Ph, or cyclohexyl), 4-Ybenzonitronylnitroxide (4-Y-PhBzNN, Y = Ph or NO 2 Ph), and 2-Z-iminonitroxide (2-Z-IN, Z = Ph or NO 2 Ph) dispersed in a polymethylmethacrylate (PMMA) matrix. The experiments were conducted at room temperature. The complex nature of the recorded ESR spectra could be attributed to the superposition of the rotational diffusion component of TEMPO (or TEMPOL) in the nanospace of the PMMA matrix with the rigid-limit component. A single component of the rigid-limit was observed for 2-X-NN and 4-Y-PhBzNN radicals dispersed in the PMMA matrix. The isotropic components of g and hyperfine (A) tensor, estimated by analyzing the solution spectra, were used to determine the g and A components of 4-Y-PhBzNN. Only the rotational diffusion component was observed for the 2-Z-IN radical. These results demonstrated that the PMMA matrix contains cylindrical nanospaces. Various radicals other than TEMPO derivatives could be used in the ESR spin probe technique as probe molecules for determining the structures, sizes, and shapes of the nanospaces.

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The protective effect of nitronyl nitroxide radical on peroxidation of A549 cell damaged by iron overload

Cited by 11 publications

References 32 publications

Iron Metabolism and Idiopathic Pulmonary Arterial Hypertension: New Insights from Bioinformatic Analysis

Iron Metabolism and Idiopathic Pulmonary Arterial Hypertension: New Insights from Bioinformatic Analysis

Synergistic effects of lipopolysaccharide and rotenone on dopamine neuronal damage in rats

Investigation of Various Organic Radicals Dispersed in Polymethylmethacrylate Matrices Using the Electron Spin Resonance Spectroscopy Technique

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