The protective effect of non-invasive low intensity pulsed electric field and fucoidan in preventing oxidative stress-induced motor neuron death via ROCK/Akt pathway

Abstract

With the expansion of the aged population, it is predicted that neurodegenerative diseases (NDDs) will become a major threat to public health worldwide. However, existing therapies can control the symptoms of the diseases at best, rather than offering a fundamental cure. As for the complex pathogenesis, clinical and preclinical researches have indicated that oxidative stress, a central role in neuronal degeneration, is a possible therapeutic target in the development of novel remedies. In this study, the motor neuron-like cell line NSC-34 was employed as an experimental model in probing the effects induced by the combination of non-invasive low intensity pulsed electric field (LIPEF) and fucoidan on the H2O2-induced neuron damage. It was found that single treatment of the LIPEF could protect the NSC-34 cells from oxidative stress, and the protective effect was enhanced by combining the LIPEF and fucoidan. Notably, it was observed that single treatment of the LIPEF obviously suppressed the H2O2-enhanced expression of ROCK protein and increased the phosphorylation of Akt in the H2O2-treated NSC-34 cells. Moreover, the LIPEF can be easily modified to concentrate on a specific area. Accordingly, this technique can be used as an advanced remedy for ROCK inhibition without the drawback of drug metabolism. Therefore, we suggest the LIPEF would be a promising strategy as a treatment for motor neurodegeneration and warrant further probe into its potential in treating other neuronal degenerations.

Effect of high-frequency low-intensity pulsed electric field on protecting SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury via ERK pathway

“…Treatment of SH-SY5Y cells with 500 μM H 2 O 2 for 24 h reduced the cell viability to 52% compared to the control cells, which was used in the subsequent experiments. To examine whether H-LIPEF could produce protection and increase the cell viability against the oxidative insult, cells were pretreated with H-LIPEF for 4 h and then exposed to H 2 O 2 in the continuous administration of H-LIPEF for another 24 h. In our previous study, the low frequency LIPEF with parameters of 2 Hz, 60V/cm, and 2 ms pulse duration was used in the mouse motor neurons NSC-34 [ 12 ], but the result showed that this parameter combination is ineffective in the SH-SY5Y cells. In this study, we used H-LIPEF in our experiments, and found that the H-LIPEF stimulation using the parameters of 200 Hz, 10 V/cm, and 2 ms pulse duration produced significant protection against the oxidative insult caused by H 2 O 2 to SH-SY5Y human neural cell line.…”

“…In our previous study, non-invasive low-intensity pulsed electric field (LIPEF) was employed, in conjunction with fucoidan, in preventing oxidative stress-induced mouse motor neuron death [ 12 ]. It found that LIPEF suppressed the hydrogen peroxide (H 2 O 2 )-enhanced expression of ROCK protein and increased the phosphorylation of Akt in H 2 O 2 -treated NSC-34 cell line.…”

“…Therefore, the approach has only limited applicability, as the specific AC signal is by no means sufficient in targeting complex signal transductions in a biological process or disease treatment. By contrast, the pulsed electric field (PEF) features a composition of multiple sinusoidal subcomponents with diverse frequencies and intensities simultaneously [ 12 ]. The study points out that thanks to its multi-frequency components, PEF can facilitate significantly the development of new disease therapeutics in fulfilling different demands.…”

Effect of high-frequency low-intensity pulsed electric field on protecting SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury via ERK pathway

“…Treatment of SH-SY5Y cells with 500 µM H 2 O 2 for 24 h reduced the cell viability to 52% compared to the control cells, which was used in the subsequent experiments. To examine whether H-LIPEF could produce protection and increase the cell viability against the oxidative insult, cells were pretreated with H-LIPEF for 4 h and then exposed to H 2 O 2 in the continuous administration of H-LIPEF for another 24 h. In our previous study, the low frequency LIPEF with parameters of 2 Hz, 60V/cm, and 2 ms pulse duration was used in the mouse motor neurons NSC-34 [12], but the result showed that this parameter combination is ineffective in the SH-SY5Y cells. In this study, we used H-LIPEF in our experiments, and found that the H-LIPEF stimulation using the parameters of 200 Hz, 10 V/cm, and 2 ms pulse duration produced significant protection against the oxidative insult caused by H 2 O 2 to SH-SY5Y human neural cell line.…”

Application of non-invasive low-intensity pulsed electric field with thermal cycling-hyperthermia for synergistically enhanced anticancer effect of chlorogenic acid on PANC-1 cells

“…The method of such non-invasive strategy of LIPEF is somewhat similar to that of tumor treating fields [19], but in a non-contact approach with different waveform and field strength. Furthermore, exposure to non-invasive LIPEF can be viewed as the simultaneous interaction of multiple frequencies [20] with a biological sample due to the electrical dispersion caused by the encounter with biological dielectrics.…”