The protective role of bone morphogenetic protein-8 in the glucocorticoid-induced apoptosis on bone cells

Kósa, János P.; Kis, Adrián; Bácsi, Krisztián; Balla, Bernadett; Nagy, Zsolt; Takács, István; Szabó, Gábor; Lakatos, Péter

doi:10.1016/j.bone.2011.01.017

Cited by 23 publications

(13 citation statements)

References 29 publications

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“…Samples were analyzed using an ABI Prism 7900HT Fast Real-Time PCR System (Applied Biosystems) (15,16). Relative quantification (RQ) studies (17) were prepared from collected data (threshold cycle numbers, referred to as Ct) with ABI Prism 7900HT Sequence-Detection System (SDS) software 2.3 (Applied Biosystems).…”

Section: Polymerase Chain Reaction (Pcr) Primers and Reagent Kitsmentioning

confidence: 99%

Influence of IL13 on Periostin Secretion by Synoviocytes in Osteoarthritis

Moue

Tajika

Ishikawa

et al. 2017

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Section: Polymerase Chain Reaction (Pcr) Primers and Reagent Kitsmentioning

confidence: 99%

Influence of IL13 on Periostin Secretion by Synoviocytes in Osteoarthritis

Moue

Tajika

Ishikawa

et al. 2017

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“…Because MMP-2 and MMP-9 are the major matrix metalloproteinases (MMPs), their activity is increased in many malignant cancers. MMP-2 and MMP-9 also play key roles in tumor cell invasion and metastasis due to their ability to degrade type IV collagen, a major component of the ECM [29][30][31] .…”

Section: Wwwchinapharcom Wang Xf Et Almentioning

confidence: 99%

Icaritin suppresses the proliferation of human osteosarcoma cells in vitro by increasing apoptosis and decreasing MMP expression

Wang

2014

Acta Pharmacol Sin

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Aim: To explore whether icaritin, a prenylflavonoid derivative of the Chinese tonic herb Epimedium, could suppress the proliferation of human osteosarcoma cells in vitro, and to elucidate the mechanisms of the action. Methods: Human osteosarcoma SaOS 2 cell line was used in the present study. The proliferation of the cells was examined using MTT assay and immunofluorescence DAPI staining. Cell motility was studied with the scratch assay. Cell apoptosis was determined by Annexin V-FITC and PI double staining using flow cytometry. Western blotting and RT-PCR were used to measure the expression of mRNAs and proteins in the cells. Results: Icaritin (5-15 μmol/L) suppressed the proliferation of SaOS 2 cells in vitro in a dose-dependent manner. Furthermore, the cell motility was significantly decreased after exposure to icaritin. Moreover, icaritin (5 μmol/L) time-dependently induced the apoptosis of SaOS 2 cells, markedly suppressed MMP-2 and MMP-9 expression, upregulated caspase-3 and caspase-9 expression, and increased the level of cleaved caspase-3 in the cells. Co-exposure to the caspase-3 inhibitor zVAD-fmk (10 μmol/L) compromised the icaritininduced caspase-3 expression and apoptosis in SaOS 2 cells. Conclusion: Icaritin suppresses the proliferation of SaOS 2 human osteosarcoma cells by increasing apoptosis and downregulating MMP expression.

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“…Bone remodeling regulates the bone metabolism through the maintenance of the balance between resorption and formation. Previous studies found that GCs could inhibit bone marrow-derived mesenchymal stem cell proliferation, promote adipogenic differentiation and induce osteoblast apoptosis [ 4 , 5 , 6 ], thus breaking the balance between bone resorption and formation. The majority of GC effects depend greatly on the glucocorticoid receptor (GR).…”

Section: Introductionmentioning

confidence: 99%

Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R

Yao

Zhang

Wang

et al. 2016

IJMS

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Pigment epithelial-derived factor (PEDF) is known as a widely expressed multifunctional secreted glycoprotein whose biological actions are cell-type dependent. Recent studies demonstrated that PEDF displays cytoprotective activity in several cell types. However, it remains unknown whether PEDF is involved in glucocorticoid-induced osteoblast death. The aim of this study was to examine the role of PEDF in osteoblast survival in response to dexamethasone, an active glucocorticoid analogue, and explore the underlying mechanism. In the present study, dexamethasone (DEX) was used to induce MC3T3-E1 pre-osteoblast apoptosis. PEDF mRNA and protein levels and cell apoptosis were determined respectively. Then PEDF receptor (PEDF-R)- and lysophosphatidic acid (LPA)-related signal transductions were assessed. Here we show that DEX down-regulates PEDF expression, which contributes to osteoblast apoptosis. As a result, exogenous recombinant PEDF (rPEDF) inhibited DEX-induced cell apoptosis. We confirmed that PEDF-R was expressed on MC3T3-E1 pre-osteoblast membrane and could bind to PEDF which increased the level of LPA and activated the phosphorylation of Akt. Our results suggest that PEDF attenuated DEX-induced apoptosis in MC3T3-E1 pre-osteoblasts through LPA-dependent Akt activation via PEDF-R.

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The protective role of bone morphogenetic protein-8 in the glucocorticoid-induced apoptosis on bone cells

Cited by 23 publications

References 29 publications

Influence of IL13 on Periostin Secretion by Synoviocytes in Osteoarthritis

Influence of IL13 on Periostin Secretion by Synoviocytes in Osteoarthritis

Icaritin suppresses the proliferation of human osteosarcoma cells in vitro by increasing apoptosis and decreasing MMP expression

Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R

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