The protective role of resveratrol against sulfoxaflor‐induced toxicity in testis of adult male rats

Abstract

This work was designed to explore the protective role of resveratrol (RES) against sulfoxaflor (Sulfx)‐induced reproductive toxicity in adult male rats. The animals were divided into six groups: Control group, Sulfx treated groups (79.5 and 205 mg/kg/day), RES treated group (20 mg/kg/day), RES + Sulfx treated groups (20 mg/kg Res + 79.5 or 205 mg/kg Sulfx) orally for 28 consecutive days. Testicular samples were collected from all groups at the end of the treatment period. Tissue supernatants were isolated for oxidative stress and cellular energy parameters; tissue samples were prepared for histopathological examination. In addition, caspase‐3 activity was calculated to assess spermatogenesis. Finally, DNA laddering assay was performed to detect DNA fragmentation as a hallmark of apoptosis. Our results showed that Sulfx treatment induced a significant increase in testicular levels of MDA, NOx, GSSG and reduced GSH level and cellular energy parameters in a dose‐dependent manner compared to the control group. The results were confirmed by histopathological study which showed pathological changes in Sulfx treated groups. A significant increase in caspase 3 and DNA fragmentation was also observed. However, concomitant administration of RES to Sulfx ‐treated rats showed significant modulation against Sulfx‐induced reproductive toxicity and attenuated the biochemical, apoptotic and histopathological changes. In conclusion, our results suggest that exposure to Sulfx at the two selected doses induces testicular toxicity and these effects can be ameliorated by supplementation of RES.

Biochemical and histopathological evaluations of thiamethoxam on the male reproductive system

“…These findings indicated increased LPO and oxidative stress as a result of TMX treatment. This was in agreement with [77], who discovered that daily oral ingestion of Sulfx for 28 days at low (79.5 mg/kg) and high (205 mg/kg) doses resulted in significant increase in testicular MDA, GSSG, and NOx level versus a dosedependent drop in GSH content. [51] discovered that giving imidacloprid (22.5 mg/kg b.wt) by orogastric each day for 56 days caused testicular free radical damage, as evidenced by exceptionally high malondialdehyde (MDA) levels, a noticeable downturn in antioxidant enzyme activity, and lower glutathione (GSH) and total antioxidant capacity (TAC) levels.…”

“…11 (2022) In comparison to the control group, the current findings revealed considerable DNA damage in the treatment groups in male testis as measured by percent DNA in tail, Comet percent, tail length, tail moment, and olive moment. This was in line with the findings of [77], who discovered that Sulfx treatment caused a significant increase in testicular DNA fragmentation, indicating poor spermatogenesis [53]. On the other hand, found that TMX administration did not result in considerable sperm DNA fragmentation.…”

“…This view is supported by [78], who found adolescent histological morphology of the testicle in males treated with TMX, as evidenced by dramatic declines in spermatogenesis, reasonable tubular atrophy, and higher occurrences of spermatic giant cells. This was in line with the findings of [77], who discovered that Sulfx administration, particularly at high doses, resulted in a testis damage characterized by a decrease in testicles morphometric parameters such as the diameter and size of seminiferous tubules due to germ cell spermatogenic deterioration. In addition, [70] discovered that treating male Wistar rats with imidacloprid (45 and 90 mg/kg, b.wt) for 28 days caused oxidative stress, which resulted in histopathological deficient spermatogenesis, as evidenced by spermatocyte exhaustion and testis and epididymal deteriorating alterations.…”

Effects and possible mechanisms of combined exposure to noise and carbon monoxide on male reproductive system in rats

“…RSV has been shown to ameliorate toxicant-induced male reproductive toxicity by attenuating oxidative stress and apoptosis. 23,57 And, there is now growing evidence that PTE protects the body's organs from environmental toxins by counteracting oxidative stress. Better yet, PTE has better oral utilization relative to RSV.…”

Reproductive effects of sulfoxaflor in male Sprague Dawley rats

“…In the current study, an increment in abnormal sperm morphology and DNA damage observed after 4 weeks after SXF administration revealed that SFX has more adverse effects on male reproductive tissue. Furthermore, another way SFX affects the function of the male reproductive system is to damage DNA (Said et al 2021 ), since the morphology of sperm is organized by various autosomal and Y-specific genes (Bansal et al 2017 ).…”