The proteins of the aqueous humour

Dernouchamps, J.P.

doi:10.1007/bf00140422

Cited by 71 publications

(26 citation statements)

References 137 publications

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“…The severity in which the aqueous humor barrier is broken may increase with protein concentration (Dernouchamps, 1982). This might be due to iris and ciliary vessel dilation (Krohne and Vestre, 1987), due to the presence of the parasite or deposition of immune complexes in the iris stroma (Molleda et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Ocular alterations in dogs naturally infected by Leishmania (Leishmania) chagasi

Brito

Alves

Maia

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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Ocular conditions, anti-Leishmania antibodies and total protein of the aqueous humor were studied in dogs naturally infected by Leishmania (Leishmania) chagasi. Fifty dogs were analyzed and assigned into two groups of 25 animals each. All animals were submitted to routine ophthalmic exam. Results showed that 76% of the affected animals presented ocular signs, being uveitis the predominant. The mean of total protein in the aqueous humor of animals with uveitis was higher (P<0.05) when compared to the mean of animals with other ocular signs or no clinical sign. The anti-Leishmania chagasi antibody values in plasma were superior to those found in the aqueous humor (P<0.05).Keywords: dog, Leishmania (Leishmania) chagasi, aqueous humor, antibodies, protein RESUMO Estudaram-se as condições oculares, os anticorpos anti-Leishmania e os valores de proteína total no humor aquoso de cães infectados naturalmente por

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Section: Discussionmentioning

confidence: 99%

Ocular alterations in dogs naturally infected by Leishmania (Leishmania) chagasi

Brito

Alves

Maia

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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“…[27][28][29] This can complicate the interpretation of a comparative proteomic analysis of AH between uveitis and control samples. In our study, we focused on the proteins of low molecular weight, while especially proteins with high molecular weight seem to accumulate in the case of a compromised blood-aqueous barrier.…”

Section: Discussionmentioning

confidence: 99%

“…Under normal conditions, the protein concentration of AH is low (0.1-0.5 mg/mL) [27][28][29] ; however, during inflammation the integrity of the blood-aqueous barrier can be severely compromised, causing remarkably increased protein concentrations. [27][28][29] This can complicate the interpretation of a comparative proteomic analysis of AH between uveitis and control samples.…”

Section: Discussionmentioning

confidence: 99%

Intraocular Biomarker Identification in Uveitis Associated With Juvenile Idiopathic Arthritis

Ayuso

Boer

Byers

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To investigate the presence of biomarkers in aqueous humor (AH) from patients with uveitis associated with juvenile idiopathic arthritis (JIA).METHODS. AH (n ¼ 73) and serum (n ¼ 105) samples from 116 children were analyzed using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-ToF MS). The samples were divided into the following groups: JIA, silent chronic anterior uveitis (AU), other uveitis entities, and noninflammatory controls. Statistical biomarker identification was performed using the SELDI-ToF Biomarker Analysis Cluster Wizard followed by multivariate statistical analysis. Biochemical identification of biomarkers was performed by polyacrylamide gel protein separation, followed by liquid chromatography tandem mass spectrometry. ELISA was performed in a number of AH samples representing all four study groups.RESULTS. In the JIA group, one AH protein peak at mass/charge (m/z) 13,762 had qualitative and quantitative differences in expression compared with the other uveitis entities and the controls, but not to the group of silent chronic AU. Its quantitative expression in AH of patients with JIA and other silent chronic AU was positively associated with uveitis activity. The protein at m/z 13,762 in AH was identified as transthyretin (TTR). The TTR concentration in AH differed significantly between the study groups (P ¼ 0.006) with considerably higher TTR concentrations in JIA and silent chronic AU samples positive for m/z 13,762 than those of the other uveitis and control groups.CONCLUSIONS. TTR is a potential intraocular biomarker of JIA-associated uveitis. Its role in the pathogenesis of silent chronic AU with and without arthritis needs further investigation.

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“…Ocular inflammation, trauma and post-operative conditions may cause breakdown of the bloodaqueous barrier which results in an increase of the protein content and cell number in the aqueous (Pedersen 1975;Dernochamps 1982;Saari et al 1985).…”

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confidence: 99%