2021
DOI: 10.3390/ijms22020875
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The Proteome and Citrullinome of Hippoglossus hippoglossus Extracellular Vesicles—Novel Insights into Roles of the Serum Secretome in Immune, Gene Regulatory and Metabolic Pathways

Abstract: Extracellular vesicles (EVs) are lipid bilayer vesicles which are released from cells and play multifaceted roles in cellular communication in health and disease. EVs can be isolated from various body fluids, including serum and plasma, and are usable biomarkers as they can inform health status. Studies on EVs are an emerging research field in teleost fish, with accumulating evidence for important functions in immunity and homeostasis, but remain to be characterised in most fish species, including halibut. Pro… Show more

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Cited by 9 publications
(11 citation statements)
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“…Deimination of complement components has previously been reported for a various complement components, including some identified here, in serum and plasma from a range of species [12][13][14][15][16][17][18][19][20][21][22][23][24][25]29]. Interestingly, in bovine serum-EVs, a number of deiminated complement components has been identified, including C1q, C3, C4A, C5a, C7, C8, C9 factor B, Factor H, C4-binding protein [20], while in teleost fish serum both C3 and C4 were identified in deiminated form but in teleost serum-EVs C3 was more dominant in deiminated form, compared with C4 [12,13,16]. This indicates that deimination mediated regulation of complement-related mechanisms, including via EVs, may differ between animal species and may possibly also link to different export of PAD isoforms in EVs between species-also bearing in mind that lower in phylogeny, only one PAD form will be responsible for all deimination, while in mammals there are more PAD isoyzmes with target-specific preferences.…”
Section: Discussionsupporting
confidence: 62%
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“…Deimination of complement components has previously been reported for a various complement components, including some identified here, in serum and plasma from a range of species [12][13][14][15][16][17][18][19][20][21][22][23][24][25]29]. Interestingly, in bovine serum-EVs, a number of deiminated complement components has been identified, including C1q, C3, C4A, C5a, C7, C8, C9 factor B, Factor H, C4-binding protein [20], while in teleost fish serum both C3 and C4 were identified in deiminated form but in teleost serum-EVs C3 was more dominant in deiminated form, compared with C4 [12,13,16]. This indicates that deimination mediated regulation of complement-related mechanisms, including via EVs, may differ between animal species and may possibly also link to different export of PAD isoforms in EVs between species-also bearing in mind that lower in phylogeny, only one PAD form will be responsible for all deimination, while in mammals there are more PAD isoyzmes with target-specific preferences.…”
Section: Discussionsupporting
confidence: 62%
“…Plasma EVs were assessed for morphology using TEM, using a pool of plasma EVs from five animals. The procedure was similar as previously described [16,20]. Following thawing of isolated EV pellets (stored frozen for 1 week before imaging), the EVs were resuspended in 100 mM sodium cacodylate buffer (pH 7.4).…”
Section: Transmission Electron Microscopy (Tem)mentioning
confidence: 99%
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