We study the helicity distributions of light flavor quark-antiquark (qq) pairs in the nucleon sea. The valence quarks are handled by adopting the light-cone SU(6) quark-spectator-diquark model and the sea qq pairs are treated from statistical consideration by introducing the helicity suppression factors lq(x) andlq(x) to parametrize the helicity distributions of q-flavor sea quark and antiquark respectively, while ∆lq(x) = lq(x) −lq(x) represents a combined effect of helicity contribution due to sea qq pairs. From fitting the nucleon polarization asymmetries A N 1 in inclusive deep inelastic scattering processes and the single-spin asymmetries A W ± L in Drell-Yan type processes, we find a significant asymmetry between the quark and antiquark helicity distributions of the nucleon sea. Therefore the quark-antiquark asymmetry of helicity distributions of nucleon sea qq pairs, i.e., ∆qs(x) = ∆qs(x), plays an important role for a comprehensive understanding of the nucleon spin content.