The Pseudomonas Quinolone Signal (PQS): Not Just for Quorum Sensing Anymore

Lin, Jie; Cheng, Jimin; Wang, Yao; Shen, Xihui

doi:10.3389/fcimb.2018.00230

Cited by 209 publications

(211 citation statements)

References 91 publications

(174 reference statements)

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“…Virulent P. aeruginosa strains repulse healthy swarms. The importance of PQS in host pathogenesis (30)(31)(32) suggests a role for the stress response observed here during human infection. We investigated the potential role of the PQS-mediated repulsion response in the Liverpool epidemic strain LESB58, which is a hypervirulent isolate of P. aeruginosa (51).…”

Section: Resultsmentioning

confidence: 78%

“…aeruginosa regulates expression of virulence genes and group behaviors through a hierarchical QS network (29). The Pseudomonas quinolone signal 2-heptyl-3-hydroxy-4-quinolone (PQS) is a molecule secreted by P. aeruginosa that has diverse roles, including mediating cell-to-cell signaling through QS, regulating virulence factor expression, iron acquisition, inducing both oxidative stress and an antioxidative response, and modulating host immune responses (30,31). PQS is found in the lungs of cystic fibrosis patients, indicating the important role of this molecule in long-term persistence of P. aeruginosa infection (32).…”

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confidence: 99%

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PQS Produced by the Pseudomonas aeruginosa Stress Response Repels Swarms Away from Bacteriophage and Antibiotics

et al. 2019

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We investigate the effect of bacteriophage infection and antibiotic treatment on the coordination of swarming, a collective form of flagellum-and pilus-mediated motility in bacteria. We show that phage infection of the opportunistic bacterial pathogen Pseudomonas aeruginosa abolishes swarming motility in the infected subpopulation and induces the release of the Pseudomonas quinolone signaling molecule PQS, which repulses uninfected subpopulations from approaching the infected area. These mechanisms have the overall effect of limiting the infection to a subpopulation, which promotes the survival of the overall population. Antibiotic treatment of P. aeruginosa elicits the same response, abolishing swarming motility and repulsing approaching swarms away from the antibiotic-treated area through a PQS-dependent mechanism. Swarms are entirely repelled from the zone of antibiotic-treated P. aeruginosa, consistent with a form of antibiotic evasion, and are not repelled by antibiotics alone. PQS has multiple functions, including serving as a quorum-sensing molecule, activating an oxidative stress response, and regulating the release of virulence and host-modifying factors. We show that PQS serves additionally as a stress warning signal that causes the greater population to physically avoid cell stress. The stress response at the collective level observed here in P. aeruginosa is consistent with a mechanism that promotes the survival of bacterial populations. IMPORTANCE We uncover a phage-and antibiotic-induced stress response in the clinically important opportunistic pathogen Pseudomonas aeruginosa. Phage-infected P. aeruginosa subpopulations are isolated from uninfected subpopulations by the production of a stress-induced signal. Activation of the stress response by antibiotics causes P. aeruginosa to physically be repelled from the area containing antibiotics altogether, consistent with a mechanism of antibiotic evasion. The stress response observed here could increase P. aeruginosa resilience against antibiotic treatment and phage therapy in health care settings, as well as provide a simple evolutionary strategy to avoid areas containing stress.

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Section: Resultsmentioning

confidence: 78%

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confidence: 99%

PQS Produced by the Pseudomonas aeruginosa Stress Response Repels Swarms Away from Bacteriophage and Antibiotics

et al. 2019

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“…9,10 PQS as well as other QS-associated secondary metabolites of P aeruginosa do not only affect the pathogen itself but also other bacterial and host cells. 11 For example, PQS was demonstrated to repress growth of other bacteria, likely by an iron-chelating activity. [12][13][14] A reduction in oxygen consumption was also observed.…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I _Q site of mitochondrial complex I

Rieger

Thierbach²,

Ommer-Bläsius

et al. 2020

FASEB BioAdvances

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Pseudomonas aeruginosa is a Gram‐negative bacterium of the proteobacteria class, and one of the most common causes of nosocomial infections. For example, it causes chronic pneumonia in cystic fibrosis patients. Patient sputum contains 2‐heptyl‐4‐hydroxyquinoline N‐oxide [HQNO] and Pseudomonas quorum sensing molecules such as the Pseudomonas quinolone signal [PQS]. It is known that HQNO inhibits the enzyme activity of mitochondrial and bacterial complex III at the Qi (quinone reduction) site, but the target of PQS is not known. In this work we have shown that PQS has a negative effect on mitochondrial respiration in HeLa and A549 cells. It specifically inhibits the complex I of the respiratory chain. In vitro analyses showed a partially competitive inhibition with respect to ubiquinone at the IQ site. In competing studies with Rotenone, PQS suppressed the ROS‐promoting effect of Rotenone, which is typical for a B‐type inhibitor. Prolonged incubation with PQS also had an effect on the activity of complex III.

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“…In comparison to standard Pseudomonas aeruginosa strain (PAO1), PhelS10 produced 2.1 times higher PQS. These signaling systems create a global regulatory network and are believed to regulate the expression of up to 12% of the genome of species belonging to P. aeruginosa, including genes related to biofilm production (Lin et al, 2018). Indeed, PhelS10 produced 22% more biofilm than strain PAO1 (Fig 2).…”

Section: Resultsmentioning

confidence: 99%

An endophytic bacteria (Pseudomonas aeruginosa) strain translocated and protected tomato (Solanum lycopersicum L.) plants from its parasitic weed Phelipanche aegyptiaca

Iasur-Kruh

Nassar

Lidor

et al. 2019

Preprint

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Phelipanche aegyptiaca is an obligate holo-parasitic weed lacking a functional photosynthetic system, which subsists on roots of a wide range of host crops, causing severe losses in yield quality and quantity. The parasite and its host are connected through their vascular system, forming a unique ecological system that enables the exchange of various substances. In a previous study, it was suggested that endophytic bacteria, which naturally inhabit the internal tissues of plants, can also be transmitted from the parasitic weed to its host and vice versa.In the current study, we investigate the characteristics of a previously isolated Pseudomonas sp. PhelS10 strain, using both biochemical and molecular methods. Our results revealed that production of Pseudomonas aeruginosa quinolone signal (PQS) was 2.1 times higher than that of the standard Pseudomonas aeruginosa strain (PAO1), which contributed to a 22% higher biofilm formation capability. PhelS10 strain was detected in the xylem of tomato plants using FISH analysis. In addition, PhelS10 strain was found in the parasitic weed's inner tissues, confirming the hypothesis that endophytic bacteria traffic between the plant host and its parasitic weed.

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The Pseudomonas Quinolone Signal (PQS): Not Just for Quorum Sensing Anymore

Cited by 209 publications

References 91 publications

PQS Produced by the Pseudomonas aeruginosa Stress Response Repels Swarms Away from Bacteriophage and Antibiotics

PQS Produced by the Pseudomonas aeruginosa Stress Response Repels Swarms Away from Bacteriophage and Antibiotics

Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I _Q site of mitochondrial complex I

An endophytic bacteria (Pseudomonas aeruginosa) strain translocated and protected tomato (Solanum lycopersicum L.) plants from its parasitic weed Phelipanche aegyptiaca

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The Pseudomonas Quinolone Signal (PQS): Not Just for Quorum Sensing Anymore

Cited by 209 publications

References 91 publications

PQS Produced by the Pseudomonas aeruginosa Stress Response Repels Swarms Away from Bacteriophage and Antibiotics

PQS Produced by the Pseudomonas aeruginosa Stress Response Repels Swarms Away from Bacteriophage and Antibiotics

Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I Q site of mitochondrial complex I

An endophytic bacteria (Pseudomonas aeruginosa) strain translocated and protected tomato (Solanum lycopersicum L.) plants from its parasitic weed Phelipanche aegyptiaca

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Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I _Q site of mitochondrial complex I