The putative cell cycle gene, enhancer of rudimentary, encodes a highly conserved protein found in plants and animals

Gelsthorpe, Mark E.; Pulumati, Malini R.; McCallum, Carrol; Dang-Vu, Khoi; Tsubota, Stuart I.

doi:10.1016/s0378-1119(96)00701-9

Cited by 43 publications

(81 citation statements)

References 24 publications

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“…In solution, purifi ed ERH protein forms homodimers, suggesting ERH might function as a dimer in cell. The Drosophila E(r) protein can be phosphorylated by casein kinase II (CKII) in vitro (Gelsthorpe et al, 1997), and mutations in E(r) at two CKII consensus sites T18 and S24 compromise E(r) function in vivo (Gelsthorpe et al, 2006). Although it remains to be determined whether CKII also phosphorylates ERH in mammalian cells, mass-spectrometry data indicate that S24 might be a major phosphorylation site on human ERH.…”

Section: Structure and Regulation Of Erhmentioning

confidence: 99%

The enigmatic ERH protein: its role in cell cycle, RNA splicing and cancer

Luo

2013

Protein Cell

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Enhancer of rudimentary homolog (ERH) is a small, highly conserved protein among eukaryotes. Since its discovery nearly 20 years ago, its molecular function has remained enigmatic. It has been implicated to play a role in transcriptional regulation and in cell cycle. We recently showed that ERH binds to the Sm complex and is required for the mRNA splicing of the mitotic motor protein CENP-E. Furthermore, cancer cells driven by mutations in the KRAS oncogene are particularly sensitive to RNAi-mediated suppression of ERH function, and ERH expression is inversely correlated with survival in colorectal cancer patients whose tumors harbor KRAS mutation. These recent fi ndings indicate that ERH plays an important role in cell cycle through its mRNA splicing activity and is critically required for genomic stability and cancer cell survival.

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Section: Structure and Regulation Of Erhmentioning

confidence: 99%

The enigmatic ERH protein: its role in cell cycle, RNA splicing and cancer

Luo

2013

Protein Cell

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“…3 ER is a highly conserved protein that has been found in plants, animals and protists, but has yet to be found in fungi. 2 ER is 76% identical to the ER homologue, ERH, of vertebrates, 49% identical to the C. elegans ERH and 40% identical to the plant (Arabidopsis) ERH. 2 The vertebrate ER homologues are very highly conserved.…”

Section: Introductionmentioning

confidence: 98%

“…1,2 An examination of the amino acid sequence does not reveal any motifs that indicate a putative function or mode of regulation of the protein other than two conserved casein kinase II sites. 3 A mutagenic analysis of these sites indicate that they are important in the regulation of the activity of ER.…”

Section: Introductionmentioning

confidence: 99%

Interactions between enhancer of rudimentary and Notch and deltex reveal a regulatory function of enhancer of rudimentary in the Notch signaling pathway in Drosophila melanogaster

Tsubota

Vogel

Phillips

et al. 2011

fly

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“…The high conservation of e(r) suggests that it encodes a vital function, 3 however previous mutagenic analyses revealed that e(r) hypomorphic mutations were viable, fertile, and morphologically wild-type and provide no compelling argument for the high conservation of the gene. 2,7 Data from the current study indicate that in Drosophila melanogaster, deficiencies of the e(r) gene are viable and morphologically wild-type, but that the null males and females have a decreased viability and that the females have a decreased fertility.…”

Section: Discussionmentioning

confidence: 96%

“…2 Subsequent studies revealed that the gene is highly conserved, present in species as diverse as insects, vertebrates, higher plants, and slime molds. 3 Current BLAST 4 searches have also identified the gene in protists, nematodes, flatworms, but not yeasts (S. I. Tsubota, unpublished data). Studies in Xenopus revealed that ER is nuclearly localized and binds to the transcription factor, DCoH/PCD (dimerization cofactor of HNF1/pterin-4a-carbinolamine dehydratase), and thus may be acting as a transcription cofactor of the HNF1 homeobox transcription factor.…”

Section: Introductionmentioning

confidence: 99%

The Generation and Analysis of Deficiencies Within a Small Genomic rRegion on the X Chromosome ofDrosophila melanogasterContaining Two Genes,enhancer of rudimentaryandCG15352

Roykhman

Ibach²,

Harding³

et al. 2007

Fly

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KEy WoRDS enhancer of rudimentary, CG15352, P element mutagenesis ABBREVIATIoNS e(r) enhancer of rudimentary r rudimentary AcKNoWlEDGEMENTSThis work was supported by grants from the National Institutes of Health (R15 GM64364), Saint Louis University, the Monsanto Company (Monsanto Scholars Program) and SUNY Brockport. ABSTRAcTThe enhancer of rudimentary gene, e(r), encodes a 104-amino-acid, highly conserved transcription cofactor. Hypomorphic mutations of e(r) show an enhancement of a hypomorphic rudimentary mutant wing phenotype. These mutants in a wild-type background are viable, fertile, and morphologically wild-type. Since the only mutant alleles were hypomorphic, it was important to isolate null mutations to determine if any other phenotypes might be associated with a loss-of-function of e(r). We utilized a marked P element, P{SUPor-P, y + }, located 895 bp upstream of the start of transcription of e(r) to generate nineteen deficiencies in the region. Deficiencies of e(r) enhance the mutant wing phenotype of a hypomorphic rudimentary allele, r hd1 . In a wild-type background, the deficiencies of e(r), unlike the hypomorphic alleles, have a low viability and females have low fertility. The expression of e(r) in the nurse cells of the ovary is consistent with the low fertility, and suggests an ovarian function for e(r). Deficiencies of CG15352, the gene directly upstream of e(r), are not associated with any obvious mutant phenotypes and present the possibility that it encodes a nonvital or redundant function.

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The putative cell cycle gene, enhancer of rudimentary, encodes a highly conserved protein found in plants and animals

Cited by 43 publications

References 24 publications

The enigmatic ERH protein: its role in cell cycle, RNA splicing and cancer

The enigmatic ERH protein: its role in cell cycle, RNA splicing and cancer

Interactions between enhancer of rudimentary and Notch and deltex reveal a regulatory function of enhancer of rudimentary in the Notch signaling pathway in Drosophila melanogaster

The Generation and Analysis of Deficiencies Within a Small Genomic rRegion on the X Chromosome ofDrosophila melanogasterContaining Two Genes,enhancer of rudimentaryandCG15352

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