The putative transcriptional regulator STM14_3563 facilitates Salmonella Typhimurium pathogenicity by activating virulence-related genes

Song, Xiaorui; Zhang, Huan; Liu, Xiaoqian; Yuan, Jian; Wang, Peisheng; Lv, Runxia; Yang, Bin; Huang, Di; Jiang, Lingyan

doi:10.1007/s10123-019-00110-3

Cited by 3 publications

(6 citation statements)

References 44 publications

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“…Available studies have confirmed that the transcription factor is an essential regulatory molecule in ST, regulating virulence and environmental stress-related gene expression ( Song et al 2020 ). Among them, the members of LysR family are the transcription factors widely found in prokaryotes ( Yang et al 2020 ), playing important regulatory roles in the life activities of bacteria.…”

Section: Discussionmentioning

confidence: 99%

A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation

Ning

et al. 2021

Polish Journal of Microbiology

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Salmonella enterica subsp. enterica serovar Typhimurium (ST) is an intracellularly parasitic bacterium. This zoonotic pathogen causes food poisoning and thus imposes a severe threat to food safety. Here, to understand the regulatory roles of the novel transcription factor STM0859 on the response of ST to environmental stress and biofilm formation, the STM0859 gene-deficient strain and the complementation strain ΔSTM0859/STM0859 were generated, respectively. Then, its capacity of responding to environmental stresses and biofilm (BF) formation ability under different stresses, including acid, alkali, high salt, cholate, and oxidative stresses was tested. We further analyzed the interaction between the STM0859 protein and the promoter of the acid stress response-related gene rcsB by performing an electrophoresis mobility shift assay (EMSA). The results showed that acid resistance and BF formation capacities of ST-ΔSTM0859 strain were significantly weaker, as compared with those of Salmonella Typhimurium SL1344 (ST-SL1344) wild strain (p < 0.01). Quantitative qRT-PCR analysis showed that the expression levels of acid stress and BF formation-related genes, rcsB and rpoS, of ST-ΔSTM0859 strain were significantly reduced at the transcription levels, while the transcription levels of these genes were fully restored in complementation strain ST-ΔSTM0859/STM0859. The results of EMSA showed that STM0859 was capable of binding the promoter DNA fragments of the rcsB gene, suggesting that STM0859 can promote the transcription of the rcsB gene through interaction with its promoter, thereby exerting an indirectly regulatory role on the adaptive responses to acid stress and BF formation of ST. This study provided new insights into the regulatory mechanisms of the LysR family factors on the tolerances of ST under adverse environmental stresses.

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Section: Discussionmentioning

confidence: 99%

A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation

Ning

et al. 2021

Polish Journal of Microbiology

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“…Furthermore, in S. Typhimurium, SPI-3, SPI-6, SPI-11, SPI-12, SPI-13, and SPI-16 are responsible for pathogen survivability inside multinuclear cells and induction of a general systemic infection [38]. SPI-4 codes the siiA-F genes involved in intestine infection caused by Salmonella and the non-fibrous adhesion protein SiiE [37]. SPI-5 (similar to SPI-1) is associated with membrane wrinkling and activation of pro-inflammatory response [12,38], whereas in S. Typhi, SPI-7 transfers the Vi capsular antigen biosynthesis gene, which is a crucial virulence factor of typhoid fever [46].…”

Section: Salmonella Pathogenesismentioning

confidence: 99%

“…SPI-5 (similar to SPI-1) is associated with membrane wrinkling and activation of pro-inflammatory response [12,38], whereas in S. Typhi, SPI-7 transfers the Vi capsular antigen biosynthesis gene, which is a crucial virulence factor of typhoid fever [46]. For SPI-14 encoded in Salmonella, the virulence-regulating protein LoiA, belonging to the LysR family (LTTR, LysR-type transcriptional regulator), plays an essential role in pathogen adhesion to the intestinal epithelium [37,40]. Furthermore, SPI-14 is responsible for the regulation of SPI-1 genes [38].…”

Section: Salmonella Pathogenesismentioning

confidence: 99%

“…Expression of SPI-1 genes is induced under the influence of environmental stimuli present in the gastrointestinal tract, i.e., low oxygen levels, high osmolarity, and neutral pH [ 33 ]. SPI-2 enables survival and growth of the pathogen in macrophages, leading to a general systemic condition [ 37 , 39 ]. SPI-2 is also responsible for SCV location and inhibits the fusion of lysosomes and SCV [ 40 ].…”

Section: Salmonella Pathogenesismentioning

confidence: 99%

“…SPI-2 contains genes that code effector, chaperones, and translocon proteins [ 12 ] inducted under the influence of environmental stimuli present in macrophages; low levels of inorganic phosphate (Pi) and Mg 2+ ions, as well as lightly acidic pH [ 33 ]. Moreover, SPI-2 genes codes a type III secretion system (similar to SPI-1) and transfers over 30 unique protein effectors to host cells during infection [ 37 ]. These proteins play an important role in protecting the pathogen against the immune response of the host by modification of intracellular vacuoles [ 51 ].…”

Section: Salmonella Pathogenesismentioning

confidence: 99%

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Transcriptional Regulation of the Multiple Resistance Mechanisms in Salmonella—A Review

et al. 2021

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The widespread use of antibiotics, especially those with a broad spectrum of activity, has resulted in the development of multidrug resistance in many strains of bacteria, including Salmonella. Salmonella is among the most prevalent causes of intoxication due to the consumption of contaminated food and water. Salmonellosis caused by this pathogen is pharmacologically treated using antibiotics such as fluoroquinolones, ceftriaxone, and azithromycin. This foodborne pathogen developed several molecular mechanisms of resistance both on the level of global and local transcription modulators. The increasing rate of antibiotic resistance in Salmonella poses a significant global concern, and an improved understanding of the multidrug resistance mechanisms in Salmonella is essential for choosing the suitable antibiotic for the treatment of infections. In this review, we summarized the current knowledge of molecular mechanisms that control gene expression related to antibiotic resistance of Salmonella strains. We characterized regulators acting as transcription activators and repressors, as well as two-component signal transduction systems. We also discuss the background of the molecular mechanisms of the resistance to metals, regulators of multidrug resistance to antibiotics, global regulators of the LysR family, as well as regulators of histone-like proteins.

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The LysR-Type Transcription Regulator YhjC Promotes the Systemic Infection of Salmonella Typhimurium in Mice

Yan

et al. 2023

IJMS

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Salmonella Typhimurium is a Gram-negative intestinal pathogen that can infect humans and a variety of animals, causing gastroenteritis or serious systemic infection. Replication within host macrophages is essential for S. Typhimurium to cause systemic infection. By analyzing transcriptome data, the expression of yhjC gene, which encodes a putative regulator in S. Typhimurium, was found to be significantly up-regulated after the internalization of Salmonella by macrophages. Whether yhjC gene is involved in S. Typhimurium systemic infection and the related mechanisms were investigated in this study. The deletion of yhjC reduced the replication ability of S. Typhimurium in macrophages and decreased the colonization of S. Typhimurium in mouse systemic organs (liver and spleen), while increasing the survival rate of the infected mice, suggesting that YhjC protein promotes systemic infection by S. Typhimurium. Furthermore, by using transcriptome sequencing and RT-qPCR assay, the transcription of several virulence genes, including spvD, iroCDE and zraP, was found to be down-regulated after the deletion of yhjC. Electrophoretic mobility shift assay showed that YhjC protein can directly bind to the promoter region of spvD and zraP to promote their transcription. These findings suggest that YhjC contributes to the systemic virulence of S. Typhimurium via the regulation of multiple virulence genes and YhjC could represent a promising target to control S. Typhimurium infection.

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The putative transcriptional regulator STM14_3563 facilitates Salmonella Typhimurium pathogenicity by activating virulence-related genes

Cited by 3 publications

References 44 publications

A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation

A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation

Transcriptional Regulation of the Multiple Resistance Mechanisms in Salmonella—A Review

The LysR-Type Transcription Regulator YhjC Promotes the Systemic Infection of Salmonella Typhimurium in Mice

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