The Pyoverdins of Pseudomonas syringae and Pseudomonas cichorii

Bultreys, Alain; Gheysen, Isabelle; Wathelet, Bernard; Schäfer, Mathias; Budzikiewicz, H.

doi:10.1515/znc-2004-9-1001

Cited by 22 publications

(23 citation statements)

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“…When the first amino acid is lysine bound with its e-amino group to the chromophore (pyoverdin 11) (Bultreys et al 2004) the A 1 fragment (m/z 457, -NH 3 m/z 440, B o m/z 357) is of medium abundance, otherwise the fragmentation corresponds to that described above for Suca/Suc side chains, (RDA-NH 3 m/z 286; m/z 256) resulting in an abundant m/z 204 ion (Fig. 5).…”

Section: Resultsmentioning

confidence: 99%

Characterization of the chromophores of pyoverdins and related siderophores by electrospray tandem mass spectrometry

et al. 2006

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Characteristic fragment ions of the various chromophores of the pyoverdin siderophore family obtained by collision activated dissociation of the [M+2H]2+ ions are reported allowing unambiguous identification. Tandem mass spectrometrical studies revealed the existence of the first example of a ferribactin with a succinamide side chain, and they add some information to the problem in which way a malic acid side chain is attached to the chromophore.

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Section: Resultsmentioning

confidence: 99%

Characterization of the chromophores of pyoverdins and related siderophores by electrospray tandem mass spectrometry

et al. 2006

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“…syringae B301D (18) and present in numerous Pseudomonas syringae strains, including P. syringae pv. tomato LMG5093 (13,15). This Pvd pss contains the 2,3-diamino-6,7-dihydroxy-quinoline chromophore characteristic of all Pvd in addition to a heptapeptide consisting of two ␤-hydroxyaspartic acids, two serines, two threonines and one lysine (18).…”

Section: Vol 189 2007 Salicylic Acid and Siderophores In Dc3000 Patmentioning

confidence: 99%

Salicylic Acid, Yersiniabactin, and Pyoverdin Production by the Model PhytopathogenPseudomonas syringaepv. tomato DC3000: Synthesis, Regulation, and Impact on Tomato andArabidopsisHost Plants

2007

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A genetically tractable model plant pathosystem, Pseudomonas syringae pv. tomato DC3000 on tomato and Arabidopsis thaliana hosts, was used to investigate the role of salicylic acid (SA) and iron acquisition via siderophores in bacterial virulence. Pathogen-induced SA accumulation mediates defense in these plants, and DC3000 contains the genes required for the synthesis of SA, the SA-incorporated siderophore yersiniabactin (Ybt), and the fluorescent siderophore pyoverdin (Pvd). We found that DC3000 synthesizes SA, Ybt, and Pvd under iron-limiting conditions in culture. Synthesis of SA and Ybt by DC3000 requires pchA, an isochorismate synthase gene in the Ybt genomic cluster, and exogenous SA can restore Ybt production by the pchA mutant. Ybt was also produced by DC3000 in planta, suggesting that Ybt plays a role in DC3000 pathogenesis. However, the pchA mutant did not exhibit any growth defect or altered virulence in plants. This lack of phenotype was not attributable to plant-produced SA restoring Ybt production, as the pchA mutant grew similarly to DC3000 in an Arabidopsis SA biosynthetic mutant, and in planta Ybt was not detected in pchA-infected wild-type plants. In culture, no growth defect was observed for the pchA mutant versus DC3000 for any condition tested. Instead, enhanced growth of the pchA mutant was observed under stringent iron limitation and additional stresses. This suggests that SA and Ybt production by DC3000 is costly and that Pvd is sufficient for iron acquisition. Further exploration of the comparative synthesis and utility of Ybt versus Pvd production by DC3000 found siderophore-dependent amplification of ybt gene expression to be absent, suggesting that Ybt may play a yet unknown role in DC3000 pathogenesis.

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“…The few examples which have been encountered so far concern the pyoverdine of Pseudomonas aeruginosa strain R 0 which has one Gln residue in its peptide chain (Ruangviriyachai et al 2001), while strain P. aeruginosa R produces an almost identical pyoverdine, but with two Gln residues in its peptide chain (Gipp et al 1991). Another example concerns Pseudomonas cichorii pyoverdine which has a Gly residue in its peptidic part replaced by a Ser residue in the Pseudomonas syringae pyoverdine, all the other components being strictly identical within the two compounds (Bultreys et al 2004). These discrete changes occurring at the level of neutral aminoacyl residues do not affect the overall charges of the molecules and thus did not affect their IEF patterns.…”

Section: Introductionmentioning

confidence: 99%

Siderotyping of fluorescent Pseudomonas: molecular mass determination by mass spectrometry as a powerful pyoverdine siderotyping method

et al. 2007

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The numerous pyoverdines so far characterized as siderophores of fluorescent Pseudomonas could be usually differentiated one from each others by the two physico-chemical and physiological methods of siderotyping, i.e., siderophore-isoelectrofocusing and siderophore-mediated iron uptake. As shown in the present paper, the structural diversity of the peptide chain characterizing these molecules results in a very large panel of molecular masses representing 64 different values ranging from 889 to 1,764 Da for the 68 compounds included in the study, with only a few structurally different compounds presenting an identical molecular mass. Thus, the molecular mass determination of pyoverdines through mass spectrometry could be used as a powerful siderotyping method.

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The Pyoverdins of Pseudomonas syringae and Pseudomonas cichorii

Cited by 22 publications

References 1 publication

Characterization of the chromophores of pyoverdins and related siderophores by electrospray tandem mass spectrometry

Characterization of the chromophores of pyoverdins and related siderophores by electrospray tandem mass spectrometry

Salicylic Acid, Yersiniabactin, and Pyoverdin Production by the Model PhytopathogenPseudomonas syringaepv. tomato DC3000: Synthesis, Regulation, and Impact on Tomato andArabidopsisHost Plants

Siderotyping of fluorescent Pseudomonas: molecular mass determination by mass spectrometry as a powerful pyoverdine siderotyping method

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