The Quality of Porcine Mesenchymal Stem Cells and Their Osteo- and Adipogenic Cell Derivatives – The Level of Proapoptotic Bad Protein Expression / Jakość Mezenchymalnych Komórek Macierzystych Świni Oraz Ich Pochodnych Zróżnicowanych W Kierunku Komórek Szeregu Osteo- I Adipogennego – Poziom Ekspresji Proapoptotycznego Białka Bad

Opiela, Jolanta; Bartel, Żaneta; Romanek, Joanna; Wieczorek, J.; Wilczek, Piotr

doi:10.2478/aoas-2013-0050

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…In turn, the osteogenic differentiation of MSCs may involve TIMP-2, MEF2a and TAZ. Simultaneously, no significant differences have been detected in the semi-quantitative profile of proapoptotic Bcl-2-associated death promoter (BAD) protein between porcine BM-MSCs, and their osteogenic and adipogenic derivatives (Opiela et al, 2013). Romanek et al (2017) analyzed the impact of high hydrostatic pressure (HHP) applied before cryopreservation on the survival rate, proliferative activity, multipotency extent and apoptosis incidence in porcine post-thaw MSCs.…”

Section: Immunophenotypic Cytobiochemical Cytophysiological and Molec...mentioning

confidence: 99%

Multi-faceted characterization of mesenchymal stem cells and their application as a powerful tool for agrobiotechnology, assisted reproductive technologies, and veterinary and regenerative biomedicine – a review

Romanek

Trzcińska

Samiec

2024

Annals of Animal Science

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A broad spectrum of cytologically specific immunophenotypic, biochemical, physiological and molecular attributes of neonatal and adult mesenchymal stem cells (MSCs) exists. Taking into account the biological traits of these types of multipotent stem cells, they play a pivotal role in the wide utilization of MSC-mediated engineering and MSC-based assisted reproductive technologies (ARTs) for animal production, veterinary research, regenerative and reconstructive medicine, and cell/tissue transplantology. Consequently, this paper sought to comprehensively provide research highlights, and insightful interpretation of current goals and future targets related to the agricultural, embryological and biomedical application of MSC-assisted biotechnological strategies and MSC-dependent interdisciplinary research techniques.

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Section: Immunophenotypic Cytobiochemical Cytophysiological and Molec...mentioning

confidence: 99%

Multi-faceted characterization of mesenchymal stem cells and their application as a powerful tool for agrobiotechnology, assisted reproductive technologies, and veterinary and regenerative biomedicine – a review

Romanek

Trzcińska

Samiec

2024

Annals of Animal Science

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Trichostatin A-Mediated Epigenetic Transformation of Adult Bone Marrow-Derived Mesenchymal Stem Cells Biases theIn VitroDevelopmental Capability, Quality, and Pluripotency Extent of Porcine Cloned Embryos

Samiec

Opiela

Lipiński

et al. 2015

BioMed Research International

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The current research was conducted to explore the in vitro developmental outcome and cytological/molecular quality of porcine nuclear-transferred (NT) embryos reconstituted with adult bone marrow-derived mesenchymal stem cells (ABM-MSCs) that were epigenetically transformed by treatment with nonspecific inhibitor of histone deacetylases, known as trichostatin A (TSA). The cytological quality of cloned blastocysts was assessed by estimation of the total cells number (TCN) and apoptotic index. Their molecular quality was evaluated by real-time PCR-mediated quantification of gene transcripts for pluripotency- and multipotent stemness-related markers (Oct4, Nanog, and Nestin). The morula and blastocyst formation rates of NT embryos derived from ABM-MSCs undergoing TSA treatment were significantly higher than in the TSA-unexposed group. Moreover, the NT blastocysts generated using TSA-treated ABM-MSCs exhibited significantly higher TCN and increased pluripotency extent measured with relative abundance of Oct4 and Nanog mRNAs as compared to the TSA-untreated group. Altogether, the improvements in morula/blastocyst yields and quality of cloned pig embryos seem to arise from enhanced abilities for promotion of correct epigenetic reprogramming of TSA-exposed ABM-MSC nuclei in a cytoplasm of reconstructed oocytes. To our knowledge, we are the first to report the successful production of mammalian high-quality NT blastocysts using TSA-dependent epigenomic modulation of ABM-MSCs.

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5. MMP-2, TIMP-2, TAZ and MEF2a Transcript Expression in Osteogenic and Adipogenic Differentiation of Porcine Mesenchymal Stem Cells

Opiela

Lipiński

Romanek

et al. 2016

Annals of Animal Science

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Mesenchymal stem cell (MSC) differentiation is regulated intrinsically by transcription factors and extrinsically by the extracellular matrix. We tested whether matrix metalloproteinase-2 (MMP-2) and its inhibitor TIMP-2, MEF2a and TAZ transcription factors are involved in porcine MSC differentiation towards adipocytes and osteocytes. Flow cytometry and immunofluorescence were used to investigate the expression levels of multipotent cell surface markers CD73 and CD105. Real-time PCR was performed to detect the osteogenic-and adipogenic-specific markers, osteocalcin and aP2, respectively, and to estimate the MMP-2, TIMP-2, MEF2a and TAZ transcript expression levels in three groups of cell, i.e., undifferentiated MSCs, adipocytes (A) and osteocytes (O). We showed that at the transcript level, the differentiation of MSCs towards adipocyte fate may involve MMP-2, TIMP-2 and TAZ. We also show that the differentiation of MSCs toward osteocyte fate may involve TIMP-2, MEF2a and TAZ. Our research provides preliminary data on the possible role of the MMP-2, TIMP-2 and TAZ transcripts in adipogenic differentiation and of the TIMP-2, TAZ and MEF2a transcripts in the osteogenic differentiation of porcine MSCs. We report for the first time the possible involvement of MEF2a in the osteogenesis of porcine MSCs. Our work may provide additional evidence for the MMP-independent function of TIMP-2 during osteogenesis.

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Abstract: J o l a n t a O p i e l a 1 , Ż a n e t a B a r t e l 2 , J o a n n a R o m a n e k 1 , J a r o s ł a w W i e c z o r e k 1 , P i o t r W i l c z e k

Cited by 3 publications

References 20 publications

Multi-faceted characterization of mesenchymal stem cells and their application as a powerful tool for agrobiotechnology, assisted reproductive technologies, and veterinary and regenerative biomedicine – a review

Multi-faceted characterization of mesenchymal stem cells and their application as a powerful tool for agrobiotechnology, assisted reproductive technologies, and veterinary and regenerative biomedicine – a review

Trichostatin A-Mediated Epigenetic Transformation of Adult Bone Marrow-Derived Mesenchymal Stem Cells Biases theIn VitroDevelopmental Capability, Quality, and Pluripotency Extent of Porcine Cloned Embryos

5. MMP-2, TIMP-2, TAZ and MEF2a Transcript Expression in Osteogenic and Adipogenic Differentiation of Porcine Mesenchymal Stem Cells

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