The quantitation of pregnancy specific β1-glycoprotein by enzyme linked immunoassay

MacDonald, Daniel J.; Belfield, Alan; Steele, Carolyn J.; Mack, D.S.; Shah, Manoj

doi:10.1016/0009-8981(79)90184-0

Cited by 19 publications

(4 citation statements)

References 9 publications

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“…The birthweights of the infants born to these control subjects were corrected for maternal height, weight and gestation according to the tables published by Thomson et al (1968) and each had a birthweight of 210th centile of weight for gestation. hPL concentrations were assayed by radioimmunoassay and SP, concentrations by an enzyme-linked immunosorbcnt assay (Macdonald et al 1979). hPL and SP, concentrations increased with advancing gestation to a peak between 36 and 38 weeks and the peak concentrations for hPL and SP, for each individual patient was used in this analysis.…”

Section: Methodsmentioning

confidence: 99%

Human placental lactogen and pregnancy‐specific β₁‐glycoprotein in pregnant diabetic women

Pledger¹,

Lunan²,

Belfield³

1982

BJOG

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Serum placental lactogen (hPL) and pregnancy-specific PIglycoprotein (SP,) concentrations were measured in 16 insulin-dependent pregnant diabetic women and in 16 non-diabetic control subjects matched for placental weight. hPL concentrations were found to be significantly higher in diabetics with placental weight >90th centile compared with those in (i) non-diabetic controls with placental weight >90th centile and (ii) diabetics with placental weights <90th centile. SP, concentrations were higher in both diabetic groups compared with those in their respective nondiabetic controls, but no tllfference existed between the two diabetic groups. In the control subjects infant birthweights were lower in the women with small placentae, but no such difference was observed between the two diabetic groups.

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Section: Methodsmentioning

confidence: 99%

Human placental lactogen and pregnancy‐specific β₁‐glycoprotein in pregnant diabetic women

Pledger¹,

Lunan²,

Belfield³

1982

BJOG

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“…The optimal conditions for the assay of cytosol and G2 binding with anti-MS antibodies were derived by the initial experimental set-up according to MacDon ald al. [3], The standard assay was as follows: 100 pi of the cytosol or G2 (1 pg protein/ml) was allowed to bind to the wells in micro ELISA plates (M 129A from Dynatech AG, Switzerland) at 4 °C for 18-20 h. The unbound material was aspirated and the wells were washed three times (1 min each) with 200 pi of 0.01 % Tween 20 in phosphate (0.1 M) buffer saline (0.14 M) pH 7.4. The remaining binding sites in the wells were saturated with 100 pi of I % (w/v) bovine serum albu min in phosphate buffer, I h at 22 °C.…”

Section: Methodsmentioning

confidence: 99%

Quantitation of Multiple Sclerosis Specific Central Nervous System Antigens in Various Regions of MS Brains

Rastogi¹,

Clausen²,

Tourtellotte³

1985

Eur Neurol

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We have estimated the levels of partially purified multiple sclerosis (MS) specific glycoproteins (MSG2) in gray matter, white matter, spinal cord and brain stem of MS autopsy brains by an enzyme immunoassay in which antisera to a pooled MSG2 was employed. Similarly, the levels of KG2 (non-MS brain isolate corresponding to MSG2) were assayed in various topographic areas of non-MS autopsy brains. Comparison of data on MS and non-MS groups revealed that the MS specific antigens may be preferentially located in the normal appearing white matter of MS brains. The concentration of MS antigens was found to be highest in the normal appearing white matter of MS brains, followed by periplaque and finally plaque. These results indicate that the MS specific antigens are not the degraded products of MS brains. They further support the view that normal appearing white matter of MS brains should be the target tissue for the isolation of MS specific antigens.

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“…Pregnancy specific ß l glycoprotein is produced by the syncytiotrophoblasts during pregnancy, when peripheral blood concentrations greater than 400 mg/1 may occur during the last trimester (1). Additionally pregnancy Materials and Methods specific ft glycoprotein may be produced by trophoReagents blastic tumours in both men and women but under these ^ p re gnancy specific ß l glycoprotein standards -Hoechst Circumstances serum concentrations are 1/1000 of those Pharmaceuticals, Hounslow, England -A range of dilutions was • -" .…”

Section: Introductionmentioning

confidence: 99%

“…Supplied by Mercia Brocades. linked immunosorbent assay (1). Enzyme linked immuno-Diluted 1:1200 in carbonate-hydrogencarbonate buffer (pH 9.6) sorbent assay has several advantages over radioimmunobefore use · assay (4) so we wished to apply the technique to serum pregnancy specific ft glycoprotein analysis in malignancy.…”

Section: Introductionmentioning

confidence: 99%

Pregnancy Specific β1 Glycoprotein: A Comparison of Analysis by Radioimmunoassay and Enzyme Linked ImmunoSorbent Assay.Observations on Patients with Malignant Teratoma of Testis

Pledger¹,

Belfield²

1981

CCLM

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Summary: A comparison of serum pregnancy specific ß 1 glycoprotein analysis by enzyme linked immunosorbent assay and radioimmunoassay has been made. Excellent correlation was obtained between the two methods but radioimmunoassay was found to be more precise and to have a lower detection limit than enzyme linked immunosorbent assay. Using the more sensitive radioimmunoassay system, 2 out of 10 control male sera were found to contain detectable levels of pregnancy specific ß l glycoprotein. Sequential results are presented on patients with diagnosed malignant teratoma of testis who were undergoing chemotherapy.

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The quantitation of pregnancy specific β1-glycoprotein by enzyme linked immunoassay

Cited by 19 publications

References 9 publications

Human placental lactogen and pregnancy‐specific β₁‐glycoprotein in pregnant diabetic women

Human placental lactogen and pregnancy‐specific β₁‐glycoprotein in pregnant diabetic women

Quantitation of Multiple Sclerosis Specific Central Nervous System Antigens in Various Regions of MS Brains

Pregnancy Specific β1 Glycoprotein: A Comparison of Analysis by Radioimmunoassay and Enzyme Linked ImmunoSorbent Assay.Observations on Patients with Malignant Teratoma of Testis

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The quantitation of pregnancy specific β1-glycoprotein by enzyme linked immunoassay

Cited by 19 publications

References 9 publications

Human placental lactogen and pregnancy‐specific β1‐glycoprotein in pregnant diabetic women

Human placental lactogen and pregnancy‐specific β1‐glycoprotein in pregnant diabetic women

Quantitation of Multiple Sclerosis Specific Central Nervous System Antigens in Various Regions of MS Brains

Pregnancy Specific β1 Glycoprotein: A Comparison of Analysis by Radioimmunoassay and Enzyme Linked ImmunoSorbent Assay.Observations on Patients with Malignant Teratoma of Testis

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Human placental lactogen and pregnancy‐specific β₁‐glycoprotein in pregnant diabetic women

Human placental lactogen and pregnancy‐specific β₁‐glycoprotein in pregnant diabetic women