The quantitative analysis of sugars in plant extracts by ion-exchange chromatography

Noggle, G. R.; Zill, L.P.

doi:10.1016/0003-9861(52)90500-6

Cited by 22 publications

(8 citation statements)

References 9 publications

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“…In view of the well-known reaction of the borate ion with OH-rich comnpounds and its recent application in the borating of sugars in order that they may be separated on ion-exchange columns (14,18), the authors conceived and tested an hypothesis as to one essential role of boron in plants. The hypothesis is that boron combines with sugar to form a sugar-borate coiiplex (ionizable) whichl is translocated withi greater facility than are nonborated, non-ionized sugar molecules.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, in order to get a response to added sucrose it was sometimes necessary to excise the root tips from the germinating pea or lima bean seeds and to culture the tips in an oxygenated base nutrient solution for 18 hours to effect a comparatively low level of endogenous respiration. In all cases in which the root tips did respond to an exogenous source of sucrose, without exception a greater respiration (02 uptake) occurred when 5 p.p.m.…”

Section: Introductionmentioning

confidence: 99%

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The Role of Boron in the Translocation of Sucrose

1953

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Role of Boron in the Translocation of Sucrose

1953

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“…The results are shown in figure 1. Each collected fraction was analyzed for sugar with the anthrone reagent and with orcinol (7), and for radioactivity by plating aliquots on aluminum planchets and counting in a gas-flow proportional counter. The first peak through the column was not identified.…”

Section: Methodsmentioning

confidence: 99%

“…KHYM and ZILL (4) de1nonstrated that a mixture of these sugar-borate complexes could be resolved by ionexchange chromatography on strong-base anion exchange resins. Subsequently, NOGGLE and ZILL (7) showed that this procedure could be used for the quantitative analysis of the sugars found in plant extracts and wlhen this method was tried as a preparative method for separating labeled sugars prepared biosynthetically, it was found to give results that overcame many of the difficulties encountered in otlher preparative procedures. The experimental results are presented in this paper.…”

Section: Introductionmentioning

confidence: 99%

The Biosynthesis of Carbon-14-Labeled Compounds. III. The Separation and Isolation of Sugars by Ion-exchange Chromatography

Noggle

Zill

1953

Plant Physiol.

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“…The recent demonstration by KHYM and ZILL (2) that sugars could be separated by ion-exchange has opened up the possibility of making a complete separation of all the sugars present in plant extracts. The method has been adapted to the quantitative analysis of the sugars in plant extracts (5) and additional work has shown its application to preparative procedures.…”

mentioning

confidence: 99%

The Biosynthesis of Carbon-14-Labeled Compounds. II. The Chromatographic Separation of the Monosaccharides, Disaccharides, and Trisaccharides from Plant Extracts

1952

Plant Physiol.

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During the development of a method for the separation of biosynthetically prepared C14-labeled glucose and fructose (4), it was found necessary to remove sucrose from the plant extracts before the monosaccharide separation could be effected. MONTGOMERY et al. (3) used the Tiselius displacement technique for isolating 6-(a-D-glucopyranosyl)-D-glucose from an enzymatic starch hydrolyzate, and indicated in a footnote that the method could be adapted to a separation of monosaccharides and disaccharides. WHISTLER and DURSO (7) demonstrated that a mixture of charcoal and Celite (Celite 535) could be used for the column separation of monosaccharides, disaccharides, and trisaccharides. Using the former technique, we found that glucose and sucrose could be separated but that the method involved the use of three charcoal columns in series, and rather large volumes of solution were required to carry out the separation. The latter method does not need elaborate equipment and enables one to handle rather large amounts of sugar without resorting to excessively large volumes of desorbing solutions. It was found possible, by using this method to separate sucrose from the monosaccharide fraction of plant sugar extracts and, in addition, to isolate a trisaccharide fraction.The general procedure followed is that of WHISTLER and DURSO (7) and is here briefly summarized. A mixture of Darco G-60 and Celite 535 was selected as the adsorbent. Other charcoals were tried but the Darco G-60 gave the most consistent results. Equal amounts by weight of the charcoal and Celite were mixed, washed with water, filtered, and dried. The adsorbent (35 gm.) was packed dry in a glass chromatographic tube 25 mm. in diameter and 300 mm. long, the bottom of which was attached to a receiving flask, which in turn was connected to a vacuum line. The adsorbent was slowly added to the tube (vacuum on) until a column 250 mm. long was attained. During the filling process the sides of the tube were vigorously tapped to aid in packing the adsorbent. Before the addition of the sugar solution 150 ml. of water were passed through the column, the flow rate then being maintained at approximiiately 200 ml. per hour. Whistler

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The quantitative analysis of sugars in plant extracts by ion-exchange chromatography

Cited by 22 publications

References 9 publications

The Role of Boron in the Translocation of Sucrose

The Role of Boron in the Translocation of Sucrose

The Biosynthesis of Carbon-14-Labeled Compounds. III. The Separation and Isolation of Sugars by Ion-exchange Chromatography

The Biosynthesis of Carbon-14-Labeled Compounds. II. The Chromatographic Separation of the Monosaccharides, Disaccharides, and Trisaccharides from Plant Extracts

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