The Quest for High-Speed and Low-Volume Bioanalysis

Laurell, Thomas; Nilsson, Johan; Markó-Varga, György

doi:10.1021/ac053408t

Cited by 28 publications

(25 citation statements)

References 42 publications

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“…The criteria of homogeneity were established by SDS polyacrylamide gel electrophoresis (Davis, 1964;Weber & Osborn, 1969) and by twodimensional immunoelectrophoresis (Laurell, 1965).…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Highly Purified Alpha‐1‐antitrypsin

Plancot

Delacourte

Han

et al. 1977

International Journal of Peptide and Protein Research

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Highly purified human alpha‐1‐antitrypsin (phenotype MM) was obtained by an original method of preparative electrophoresis. The criteria of homogeneity were assured by one arc in crossed immunoelectrophoresis and one band on polyacrylamide gel. A unique N‐terminal amino acid (pyroglutamic acid) and a unique C‐terminal residue (lysine) were identified. Determined by gel electrophoresis, its molecular weight was 47,000 daltons.

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“…The criteria of homogeneity were established by SDS polyacrylamide gel electrophoresis (Davis, 1964;Weber & Osborn, 1969) and by twodimensional immunoelectrophoresis (Laurell, 1965).…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Highly Purified Alpha‐1‐antitrypsin

Plancot

Delacourte

Han

et al. 1977

International Journal of Peptide and Protein Research

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“…[1][2][3] Recently, the drying process of droplets containing nonvolatile materials is focused as an important problem in inkjet printing, which is attracting increasing attention as a key factor in the next-generation fabrication processes for microelectronics [4][5][6] and biomaterials. [7][8][9] A problem that needs to be considered here is the control of the thickness profiles of the solute films deposited on the substrate after the droplets totally dry up.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of Drying Process of Polymer Solution Droplets: Analysis of Polymer Transport and Control of Film Profiles

Kajiya

Doi

2011

J. Soc. Rheol., Jpn

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We report a series of experimental works about the drying process of polymer solution droplets. Our aims are to analyze the local polymer transport in the drying droplet and to precisely control the profile of polymer film formed after the droplet dries up. For the analysis of the polymer transport, we have established a dynamical observation method for the polymer concentration field by combining the fluorescence microscopy with the lateral profile observation. We observed that a large spatial inhomogeneity of the polymer concentration grows inside the droplet which is caused by the outward capillary flow. For the control of the film profile, we proposed two methods: adding the small amount of surfactant and switching between the solvent evaporation and condensation. These two processes enable us to make a polymer film having a flat profile. Our results propose effective suggestions for controlling the fabrication process of polymer films in inkjet-printing technologies.

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“…Most widely-used immobilization strategies including physical adsorption, electrostatic and hydrophobic interactions, and chemical binding have been implanted onto the PSi surface [12][13][14][15][16][17][18]. These methods share some common disadvantages such as nonspecific adsorption, decrease of biological activity of immobilized protein, and uncontrolled orientation of attachment.…”

Section: Introductionmentioning

confidence: 99%

Covalently derivatized NTA microarrays on porous silicon for multi-mode detection of His-tagged proteins

Pei

Tang

et al. 2010

Sci. China Chem.

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Porous silicon (PSi) was applied as a supporting substrate for stepwise covalent derivatization of undecylenic acid, N-hydroxysuccinimidyl ester (NHS-ester) and nitrilotriacetic acid (NTA). By taking the advantages of porous silicon as a supporting matrix such as high surface area to volume ratio, infrared transparency, porous semiconductors for laser desorption/ionization mass spectroscopy, and low fluorescence background, a multi-mode detection biochip prototype can be realized. We prepared such a protein microarray by spotting NTA microarray dots on NHS-ester derivatized PSi, converting the rest of chip area into poly(ethylene glycol) background, loading Ni II , and finally affinity-binding histidine-tagged (His-tagged) proteins. With the multi-mode analyses of infrared spectroscopy, X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), matrix-assisted laser desorption/ionization mass spectroscopy (MALDI-MS), and fluorescence scanning, two example proteins, His-tagged thioredoxin-urodilatin and His-tagged aprotinin, were well qualified and quantified. porous silicon, microarray, multi-mode, NTA, His-tagged protein

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The Quest for High-Speed and Low-Volume Bioanalysis

Cited by 28 publications

References 42 publications

Isolation and Characterization of Highly Purified Alpha‐1‐antitrypsin

Isolation and Characterization of Highly Purified Alpha‐1‐antitrypsin

Dynamics of Drying Process of Polymer Solution Droplets: Analysis of Polymer Transport and Control of Film Profiles

Covalently derivatized NTA microarrays on porous silicon for multi-mode detection of His-tagged proteins

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