The R2R3-MYB gene PsMYB58 positively regulates anthocyanin biosynthesis in tree peony flowers

Zhang, Yanzhao; Xu, Shuzhen; Ma, Huiping; Duan, Xujia; Gao, Shouxin; Zhou, Xiaojun; Cheng, Yongqiang

doi:10.1016/j.plaphy.2021.04.034

Cited by 38 publications

(19 citation statements)

References 42 publications

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“…The MYB superfamily is grouped into four subfamilies: 1R-MYB (MYB-related and R3-MYB), R2R3-MYB, 3R-MYB (R1R2R3-MYB), and 4RMYB. R2R3-MYB is the largest subfamily, with some members regulating plant growth and developmental [11], primary and secondary metabolism [12][13][14][15][16][17], response to various biotic and abiotic stresses [18][19][20][21], hormone synthesis, and signal transduction [22]. In recent years, more studies focused on the R2R3-MYB TFs regulating carotenoid metabolism.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Comparative Analysis of the R2R3-MYB Gene Family in Five Solanaceae Species and Identification of Members Regulating Carotenoid Biosynthesis in Wolfberry

Yin

Guo

Shi

et al. 2022

IJMS

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The R2R3-MYB is a large gene family involved in various plant functions, including carotenoid biosynthesis. However, this gene family lacks a comprehensive analysis in wolfberry (Lycium barbarum L.) and other Solanaceae species. The recent sequencing of the wolfberry genome provides an opportunity for investigating the organization and evolutionary characteristics of R2R3-MYB genes in wolfberry and other Solanaceae species. A total of 610 R2R3-MYB genes were identified in five Solanaceae species, including 137 in wolfberry. The LbaR2R3-MYB genes were grouped into 31 subgroups based on phylogenetic analysis, conserved gene structures, and motif composition. Five groups only of Solanaceae R2R3-MYB genes were functionally divergent during evolution. Dispersed and whole duplication events are critical for expanding the R2R3-MYB gene family. There were 287 orthologous gene pairs between wolfberry and the other four selected Solanaceae species. RNA-seq analysis identified the expression level of LbaR2R3-MYB differential gene expression (DEGs) and carotenoid biosynthesis genes (CBGs) in fruit development stages. The highly expressed LbaR2R3-MYB genes are co-expressed with CBGs during fruit development. A quantitative Real-Time (qRT)-PCR verified seven selected candidate genes. Thus, Lba11g0183 and Lba02g01219 are candidate genes regulating carotenoid biosynthesis in wolfberry. This study elucidates the evolution and function of R2R3-MYB genes in wolfberry and the four Solanaceae species.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Comparative Analysis of the R2R3-MYB Gene Family in Five Solanaceae Species and Identification of Members Regulating Carotenoid Biosynthesis in Wolfberry

Yin

Guo

Shi

et al. 2022

IJMS

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“…Our study showed that highly expressed MYB genes, such as Cluster-24474.31177, Cluster-24474.28911, Cluster-24474.26570, Cluster-24474.35504, Cluster-24474.30825, and Cluster-24474.45773, were concentrated mainly in orange and red leaves, which, in turn, may regulate key structural genes involved in anthocyanin biosynthesis positively. Similarly, the overexpression of PsMYB58 (an anthocyanin regulatory R2R3-MYB gene in tree peony flowers) in tobacco enhanced anthocyanin accumulation in various organs [58]. In contrast, we also found some MYBs (Cluster-24474.33458, Cluster-24474.28542, Cluster-24474.31177, Cluster-24474.34995, Cluster-24474.35337, and Cluster-24474.31121) that were downregulated dramatically in red leaves.…”

Section: Transcription Factors Associated With Anthocyanin Biosynthesismentioning

confidence: 59%

Metabolome and Transcriptome Analyses Unravels Molecular Mechanisms of Leaf Color Variation by Anthocyanidin Biosynthesis in Acer triflorum

et al. 2022

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Acer triflorum Komarov is an important ornamental tree, and its seasonal change in leaf color is the most striking feature. However, the quantifications of anthocyanin and the mechanisms of leaf color change in this species remain unknown. Here, the combined analysis of metabolome and transcriptome was performed on green, orange, and red leaves. In total, 27 anthocyanin metabolites were detected and cyanidin 3-O-arabinoside, pelargonidin 3-O-glucoside, and peonidin 3-O-gluside were significantly correlated with the color development. Several structural genes in the anthocyanin biosynthesis process, such as chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and dihydroflavonol 4-reductase (DFR), were highly expressed in red leaves compared to green leaves. Most regulators (MYB, bHLH, and other classes of transcription factors) were also upregulated in red and orange leaves. In addition, 14 AtrMYBs including AtrMYB68, AtrMYB74, and AtrMYB35 showed strong interactions with the genes involved in anthocyanin biosynthesis, and, thus, could be further considered the hub regulators. The findings will facilitate genetic modification or selection for further improvement in ornamental qualities of A. triflorum.

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“…Since the function of PsMYB2 had not been studied by others, we first examined its role in anthocyanin biosynthesis. Phylogenetic analyses indicated that the PsMYB2 protein was different from MYB TFs identified in tree peony recently, which participated in the regulation of anthocyanin biosynthesis, such as PsMYB57 ( Zhang et al, 2020 ), PsMYB58 ( Zhang et al, 2021 ), and PsMYB114L ( Zhang et al, 2019 ). It belonged to a small cluster including VvMYB5a/b -like TFs ( Figure 6B ).…”

Section: Discussionmentioning

confidence: 89%

Glucose Supply Induces PsMYB2-Mediated Anthocyanin Accumulation in Paeonia suffruticosa ‘Tai Yang’ Cut Flower

Zhang

Li²,

Zhang³

et al. 2022

Front. Plant Sci.

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Tree peony (Paeonia suffruticosa) is a well-known Chinese ornamental plant with showy flower color. However, the color fading problem during vase time seriously blocks its development in the cut flower market. In this study, we found that exogenous glucose supply improved the color quality of P. suffruticosa ‘Tai Yang’ cut flowers with increased total soluble sugar and anthocyanin contents of petals. Besides, the promotion effect of glucose was better than the osmotic control of 3-O-methylglucose (3OMG) treatment and the glucose analog mannose treatment. The structural genes, including PsF3H, PsF3′H, PsDFR, PsAOMT, and PsUF5GT, were remarkably upregulated under glucose treatment. Meanwhile, the regulatory genes, including PsbHLH1, PsbHLH3, PsMYB2, PsWD40-1, and PsWD40-2, also showed a strong response to glucose treatment. Among these five regulatory genes, PsMYB2 showed less response to 3OMG treatment but was highly expressed under glucose and mannose treatments, indicating that PsMYB2 may have an important role in the glucose signal pathway. Ectopic overexpression of PsMYB2 in Nicotiana tabacum resulted in a strong pigmentation in petals and stamens of tobacco flowers accompanied with multiple anthocyanin biosynthetic genes upregulated. More importantly, the overexpression of PsMYB2 enhanced the ability of glucose-induced anthocyanin accumulation in Arabidopsis thaliana seedlings since PsMYB2-overexpressing Arabidopsis showed higher expression levels of AtPAL1, AtCHS, AtF3H, AtF3′H, AtDFR, and AtLDOX than those of wild type under glucose treatment. In summary, we suggested that glucose supply promoted petal coloration of P. suffruticosa ‘Tai Yang’ cut flower through the signal pathway, and PsMYB2 was a key component in this process. Our research made a further understanding of the mechanism that glucose-induced anthocyanin biosynthesis of P. suffruticosa cut flowers during postharvest development, laying a foundation for color retention technology development of cut flowers.

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The R2R3-MYB gene PsMYB58 positively regulates anthocyanin biosynthesis in tree peony flowers

Cited by 38 publications

References 42 publications

Genome-Wide Comparative Analysis of the R2R3-MYB Gene Family in Five Solanaceae Species and Identification of Members Regulating Carotenoid Biosynthesis in Wolfberry

Genome-Wide Comparative Analysis of the R2R3-MYB Gene Family in Five Solanaceae Species and Identification of Members Regulating Carotenoid Biosynthesis in Wolfberry

Metabolome and Transcriptome Analyses Unravels Molecular Mechanisms of Leaf Color Variation by Anthocyanidin Biosynthesis in Acer triflorum

Glucose Supply Induces PsMYB2-Mediated Anthocyanin Accumulation in Paeonia suffruticosa ‘Tai Yang’ Cut Flower

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