The radioimmunoassay of pregnanediol-3α-glucuronide

Samarajeewa, Padmananda; Cooley, G.; Kellie, A. E.

doi:10.1016/0022-4731(79)90169-9

Cited by 51 publications

(12 citation statements)

References 18 publications

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“…Urine specimens were assayed for the major urinary metabolite of progesterone, pregnanediol-3-glucuronide (PdG), and for hCG. PdG was measured in duplicate or triplicate by RIA [20]. Human CG was measured in duplicate with an ultrasensitive polyclonal immunoradiometric assay designed to measure intact hCG (␣ and ␤ subunits in the dimeric form) [19,21].…”

Section: Hormone Assays and Hormonal Identification Of Ovulation And mentioning

confidence: 99%

Rescue of the Corpus Luteum in Human Pregnancy1

Baird

Weinberg

McConnaughey

et al. 2003

Biology of Reproduction

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Rescue of the corpus luteum from its programmed senescence maintains progesterone production required for pregnancy. In primates, chorionic gonadotropin produced by the developing conceptus acts as the primary luteotrophic signal. The purpose of this research was to assess corpus luteum rescue by examining changes in daily urinary progesterone metabolite levels during the first week after implantation. We determined the variability in progesterone metabolite profiles and evaluated its relationship to early pregnancy loss in 120 naturally conceived human pregnancies, including 43 early pregnancy losses. In other primates, an abrupt increase in the progesterone metabolite occurs at the time of implantation. This pattern occurred in an estimated 45% of the pregnancies in the present study. In the remaining pregnancies, there was a delayed rise (18%), neither a rise or decline (22%), or a decline (15%) during the week after implantation. The estimated rate of early pregnancy loss increased across these categories (from 5% loss with an abrupt rise at implantation to 100% loss with progesterone metabolite decline). Low urinary hCG levels in early pregnancy were significant determinants of a decline in postimplantation progesterone metabolite. However, preimplantation steroid metabolite levels were not significant, suggesting no inherent problem with the corpus luteum. Examination of individual progesterone metabolite profiles in relation to hCG profiles also indicated that few losses were caused by corpus luteum failure. Delineating the functional importance of an abrupt progesterone rise at the time of implantation may provide new strategies for promoting successful implantation in assisted reproduction. corpus luteum, corpus luteum function, human chorionic gonadotropin, pregnancy, progesterone

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Section: Hormone Assays and Hormonal Identification Of Ovulation And mentioning

confidence: 99%

Rescue of the Corpus Luteum in Human Pregnancy1

Baird

Weinberg

McConnaughey

et al. 2003

Biology of Reproduction

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“…The concentration of pregnanediol glucoronide in the urine was determined by the method of Samarajeewa et al (1979). Urinary creatinine was measured by the kinetic alkaline picrate method.…”

Section: Determination Of Ovulationmentioning

confidence: 99%

The effects of moderate physical activity on menstrual cycle patterns in adolescence: Implications for breast cancer prevention

Bernstein¹,

Ross²,

Løbo³

et al. 1987

Br J Cancer

219

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Summary Girls who engage in strenuous physical activity are often amenorrheic and have recently been reported to be at a reduced risk of breast cancer. To determine whether moderate amounts of exercise affect menstrual cycle patterns and ovulatory frequency in young postmenarcheal girls, the menstrual cycles and physical activity patterns of 168 high school girls were monitored for a 6 month period. Anovulatory cycles were associated with later age at menarche, fewer elapsed years since menarche and greater levels of energy expended per week in physical activity. After adjusting for age at menarche and years since menarche, there was a significant dose-related trend in the risk of anovular menstrual cycles associated with increasing levels of physical activity (1-sided P=0.03). Major determinants of average cycle length were weekly average energy expenditure (< 750 kcal wk-1 associated with cycles that were on average 2.4 days longer), age at menarche (an increase of 0.7 days per year of age) and race (Asians having cycles about 1.9 days longer than Caucasians). Because a major determinant of breast cancer risk may be the cumulative number of ovulatory cycles, these data suggest that regular participation in moderate physical activity, by reducing the frequency of ovulatory cycles in adolescence, may provide an opportunity for the primary prevention of breast cancer.

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“…Urine samples were stored in the domestic freezer until being taken to the laboratory, where they were stored at -20°C until required for hormonal measurements. Samples were assayed for estrone glucuronide (El-G) and pregnanediol glucuronide (Pd-3e-G), using established RIAs [13,14]. The reagents were provided by the WHO Matched Reagents Program [15].…”

Section: Sample Collection and Monitoring Of Ovarian Functionmentioning

confidence: 99%

Luteinizing hormone releasing hormone agonist for postpartum contraception

et al. 1996

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GnRH agonists have advantages over steroids for hormonal contraception during the postpartum period in breastfeeding women. Symptoms of hypoestrogenism were not reported any time in either the controls or the treated groups, as estrone levels were not suppressed to menopausal values. Once-daily administration of GnRH agonist could be a reliable, acceptable and safe contraceptive method during the postpartum period in breastfeeding women. More information is required to establish GnRH analog contraceptive efficacy.

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The radioimmunoassay of pregnanediol-3α-glucuronide

Cited by 51 publications

References 18 publications

Rescue of the Corpus Luteum in Human Pregnancy1

Rescue of the Corpus Luteum in Human Pregnancy1

The effects of moderate physical activity on menstrual cycle patterns in adolescence: Implications for breast cancer prevention

Luteinizing hormone releasing hormone agonist for postpartum contraception

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