The radioprotective effect of N-acetylcysteine against x-radiation-induced renal injury in rats

Mercantepe, Tolga; Topçu, Atilla; Rakıcı, Sema Yılmaz; Tümkaya, Levent; Yılmaz, Adnan; Mercantepe, Filiz

doi:10.1007/s11356-019-06110-0

Cited by 18 publications

(19 citation statements)

References 48 publications

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“…NAC treatments (300 mg/kg, sc), starting either 4 h prior to or 2 h after radiation exposure, and with six subsequent daily injections over 7 days, reduced early deaths in abdominally irradiated (X-rays, 20 Gy) C57BL/6 mice [ 95 ]. More recently, radioprotective effects of NAC have been demonstrated in multiple studies [ 96 , 97 ], but the use of GSH or NAC with oncoradiotherapy cannot be supported because it may also favor cancer cell metastasis and radioresistance. Erdosteine (a homocysteine derivative) is a potent free radical scavenger, increases GPx and catalase (CAT) activities and GSH intracellular levels.…”

Section: Medical Countermeasuresmentioning

confidence: 99%

Nuclear and Radiological Emergencies: Biological Effects, Countermeasures and Biodosimetry

et al. 2022

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Atomic and radiological crises can be caused by accidents, military activities, terrorist assaults involving atomic installations, the explosion of nuclear devices, or the utilization of concealed radiation exposure devices. Direct damage is caused when radiation interacts directly with cellular components. Indirect effects are mainly caused by the generation of reactive oxygen species due to radiolysis of water molecules. Acute and persistent oxidative stress associates to radiation-induced biological damages. Biological impacts of atomic radiation exposure can be deterministic (in a period range a posteriori of the event and because of destructive tissue/organ harm) or stochastic (irregular, for example cell mutation related pathologies and heritable infections). Potential countermeasures according to a specific scenario require considering basic issues, e.g., the type of radiation, people directly affected and first responders, range of doses received and whether the exposure or contamination has affected the total body or is partial. This review focuses on available medical countermeasures (radioprotectors, radiomitigators, radionuclide scavengers), biodosimetry (biological and biophysical techniques that can be quantitatively correlated with the magnitude of the radiation dose received), and strategies to implement the response to an accidental radiation exposure. In the case of large-scale atomic or radiological events, the most ideal choice for triage, dose assessment and victim classification, is the utilization of global biodosimetry networks, in combination with the automation of strategies based on modular platforms.

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Section: Medical Countermeasuresmentioning

confidence: 99%

Nuclear and Radiological Emergencies: Biological Effects, Countermeasures and Biodosimetry

et al. 2022

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“…TSPCs were highly sensitive to ROS-induced cytotoxicity by reducing their capacity of self-renewal and differentiation [ 15 , 35 , 36 ]. NAC is a potent antioxidant and has been widely used in clinical practice to improve organ damage caused by oxidative stress [ 22 – 25 ] because NAC can scavenge ROS, minimizing oxidative stress-related cell damage [ 25 , 37 ]. In this study, we found that treatment with NAC at a range of doses significantly promoted the proliferation of TSPCs in a dose-dependent manner and mitigated ROS production to preserve the survival of TSPCs in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…The application of antioxidants can alleviate local ROS accumulation and inflammation in injured tendons [ 16 , 20 , 21 ]. N-acetyl-L-cysteine (NAC) is the most common drug for neutralizing ROS to relieve OS and has been widely used in the respiratory and circulatory systems to relieve OS-related damage in cells [ 22 – 24 ]. Functionally, NAC can inhibit the generation of ROS and scavenge ROS because it can be deacetylated to form L-cysteine, which promotes the synthesis of endogenous glutathione to inhibit OS.…”

Section: Introductionmentioning

confidence: 99%

N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin α5/β1/PI3K/AKT signaling

Zhou

Wang

et al. 2023

BMC Mol and Cell Biol

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Background Tendon injury is associated with oxidative stress, leading to reactive oxygen species (ROS) production and inflammation. N-acetyl-L-cysteine (NAC) is a potent antioxidant. However, how NAC affects the biological functions of tendon stem/progenitor cells (TSPCs) and tendon repair has not been clarified. Method The impacts of NAC on the viability, ROS production, and differentiation of TSPCs were determined with the cell counting kit-8, fluorescence staining, Western blotting, and immunofluorescence. The effect of NAC on gene transcription in TSPCs was analyzed by transcriptomes and bioinformatics and validated by Western blotting. The potential therapeutic effect of NAC on tendon repair was tested in a rat model of Achilles tendon injury. Results Compared with the untreated control, treatment with 500 µM NAC greatly promoted the proliferation of TSPCs and significantly mitigated hydrogen peroxide-induced ROS production and cytotoxicity in vitro. NAC treatment significantly increased the relative protein expression of collagen type 1 alpha 1 (COL1A1), tenascin C (TNC), scleraxis (SCX), and tenomodulin (TNMD) in TPSCs. Bioinformatics analyses revealed that NAC modulated transcriptomes, particularly in the integrin-related phosphoinositide 3-kinase (PI3K)/AKT signaling, and Western blotting revealed that NAC enhanced integrin α5β1 expression and PI3K/AKT activation in TSPCs. Finally, NAC treatment mitigated the tendon injury, but enhanced the protein expression of SCX, TNC, TNMD, and COLIA1 in the injured tissue regions of the rats. Conclusion NAC treatment promoted the survival and differentiation of TSPCs to facilitate tendon repair after tendon injury in rats. Thus, NAC may be valuable for the treatment of tendon injury.

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“…Thus, after irradiation of rats at a dose of 2 Gy an imbalance in pro‐ and antioxidative processes was shown, and oxidative stress was formed in enterocytes of the rat small intestine (Garau et al, 2011; Khizhniak et al, 2013). When rodents were irradiated at a dose of 5 Gy, individual apoptotic cells were found along the “crypt‐villus” axis of the intestinal epithelium (Garau et al, 2011; Mercantepe et al, 2019). Nevertheless, these changes probably did not affect the functional state of the intestinal barrier, which did not reveal in electrophysiological characteristics and intestinal permeability under irradiation in doses less than 10 Gy in our study.…”

Section: Discussionmentioning

confidence: 99%

Dose and time dependence of functional impairments in rat jejunum following ionizing radiation exposure

et al. 2021

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The radioprotective effect of N-acetylcysteine against x-radiation-induced renal injury in rats

Cited by 18 publications

References 48 publications

Nuclear and Radiological Emergencies: Biological Effects, Countermeasures and Biodosimetry

Nuclear and Radiological Emergencies: Biological Effects, Countermeasures and Biodosimetry

N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin α5/β1/PI3K/AKT signaling

Dose and time dependence of functional impairments in rat jejunum following ionizing radiation exposure

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