The RANKL expression and osteoclast in alveolar bone of rat diabetic model at different mechanical force application

Abstract

Background: Diabetes is a serious and important public health problem, especially in relation to dental treatment. Because of its complications in periodontal tissue, diabetes can be contraindicated in patients undergoing orthodontic treatment. The receptor activator of nuclear factor-κb ligand (RANKL) is an essential cytokine inducing osteoclastogenesis. Osteoblasts produce this cytokine which has been suggested to play an integral role in osteoclast activation during bone remodeling of orthodontic tooth movement. Purpose: The aim of this study was to determine the correlation between RANKL expression of osteoblast and the number of osteoclasts in the alveolar bone of diabetic rat models at different mechanical force application. Methods: This study used animal subjects, white rats (Rattus norvegicus) of the Wistar strain (n=24) divided into six groups. The mechanical force to which they were subjected ranged between 10, 20, and 30 gramforce (grf). The animal models with diabetes were injected with a stratified dose of Streptozotocin. An orthodontic appliance was inserted in both the maxillary incisors for seven days. The tissue was subjected to histological analysis of osteoclasts and immunohistochemistry analysis of RANKL expression on the pressure and tension side of the alveolar bone. Results:The results of this study showed that the increase in mechanical force produced a rise in RANKL expression and osteoclast number on the pressure and tension side of the alveolar bone of diabetic rat models. Conclusion: There was a correlation between the RANKL of osteoblast and osteoclast numbers in the alveolar bone of diabetic models with different mechanical force application.

The effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movements

“…In addition, experimental animals were induced with Streptozotocin (SC-200719, Santa Cruz) for 5 consecutive days at doses of 40, 35, 30, 25, and 20 mg/kg BW intraperitoneally followed by incubation for 7 days. 17 Diabetes in experimental animals was characterized by blood glucose levels >300 mg/dL.…”

“…A 30-g force of orthodontic appliance was applied in line with a previous study for 7 days. 17 In diabetes cacao groups, cacao extract was given orally using a gastric probe for 7 days after the orthodontic appliance was inserted. The doses of cacao extract were 125, 250, and 500 mg/kg BW.…”

Alveolar Bone Osteoclast Profile in the Periodontitis Wistar Rats Model with the Snail Slime (Achatina Fulica) Application

“…The experimental animals were euthanised using ether anaesthesia; then the jaw was cut in the mandibular incisor region. The sample was prepared using 10% formalin for 24 hours, decalcified using 14% EDTA, for 30 days, dehydrated, cleared, and embedded in paraffin, then cut in the longitudinal direction with a thickness of 5 microns for the Hematoxylin-Eosin (HE) painting procedure [12]. Staining procedures were performed using HE to determine the histological feature of osteoclasts.…”