The rapid loss of viability ofAzotobacter in aqueous solutions

Smith, Darwin; Wyss, Orville

doi:10.1007/bf02219119

Cited by 16 publications

(2 citation statements)

References 13 publications

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“…Few data existed on centrifugation injury and they concluded that such damage was slight. Smith and Weiss (1969) and Winslow and Brooke (1927), however, reported progressive losses in the viabilities of Azotobacter spp. and Bacillus and Serratia spp.…”

Section: Introductionmentioning

confidence: 98%

Loss of salt-tolerance and transformation efficiency in Escherichia coli associated with sub-lethal injury by centrifugation

Wyber

Andrews

Gilbert

1994

Lett Appl Microbiol

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Sub-lethal injury of Escherichia coli has been detected following centrifugation at g-forces between 5 and 30 kg. The extent of injury was measured either as a reduction in colony forming ability when plated onto NaCl-containing plates (2% w/v), or as a reduction in transformation efficiency associated with plasmid pBR322 encoding ampicillin resistance. In both cases, the extent of sub-lethal injury was found to increase with increasing centrifugal force and probably reflects structural damage to the cell envelope.

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Section: Introductionmentioning

confidence: 98%

Loss of salt-tolerance and transformation efficiency in Escherichia coli associated with sub-lethal injury by centrifugation

Wyber

Andrews

Gilbert

1994

Lett Appl Microbiol

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“…Contrasting results have been reported on the effect of centrifugation on the viability of cells. Farwell and Brown (1971) concluded that centrifugation injury was in general negligible, while Smith and Weiss (1969) found a progressive loss of viability ofAzotobacter spp., in agreement with similar observations reported by Winslow and Brooke (1927) for Bacillus and Serratia spp. Gilbert et al (1990) reported that centrifugation of exponential and stationary phase cells of Escherichia coli at 10 OOOg had no effect on their viability, although some lipopolysaccharide associated with the cell envelope was shed into the medium on harvesting by centrifugation, and the susceptibility of the cells towards bisbiguanide antiseptics increased 100-fold.…”

Section: Introductionmentioning

confidence: 99%