The ras-related ypt protein is an ubiquitous eukaryotic protein: isolation and sequence analysis of mouse cDNA clones highly homologous to the yeast YPT1 gene.

Abstract

The YPT1 gene of the yeast Saccharomyces cerevisiae codes for a guanine nucleotide‐binding protein which is essential for cell viability. Using as hybridization probe cloned yeast YPT1 gene sequences, we have isolated from cDNA libraries prepared from RNA of mouse F9 and C3H10T1/2 cells several overlapping cDNA clones with identical sequence in the regions of overlap. The cDNAs were derived from a gene, designated ypt1, which codes for a protein of 205 amino acids with 71% homology to the yeast YPT1 gene product. Amino acid sequences typical for guanine nucleotide‐binding proteins and characteristic for ypt proteins are perfectly conserved in the mouse ypt1 protein. Two mRNAs of 1600 and 3200 nucleotides, originating from the mouse ypt1 gene and differing in the length of their 3′‐non‐translated region, were identified in mouse F9 cells and in all mouse tissues examined. A monoclonal antibody specifically recognizing the 23.5‐kd yeast YPT1 protein cross‐reacted with a protein of identical size on protein blots of mouse, rat, pig, bovine and human cell lines.

Identification of ras-related, YPT family genes in Schizosaccharomyces pombe.

“…Since S.pombe and S. cerevisiae are phylogenetically distantly related (Huysmans et al, 1983), these results suggest that the YPT family genes are widely conserved in eukaryotes. A gene that is 72 % homologous to Sc YPTI in amino acid sequence and can complement S. cerevisiae yptl has indeed been isolated from mouse and named yptl (Haubruck et al, 1987(Haubruck et al, , 1989. Genes encoding exactly the same amino acid sequence as mouse yptl have been isolated from a rat brain cDNA library (Touchot et al, 1987) and a human pheochromocytoma cDNA library (Zahraoui et al, 1989), and are named rabi and Hrabl respectively.…”

Tomato Rab1A Homologs as Molecular Tools for Studying Rab Geranylgeranyl Transferase in Plant Cells

“…Mouse RablA was first proposed to be a mammalian homolog of YPTlp on both the basis of its sequence similarity with YPTlp (>70% identity) and its ability to replace YPTlp in yeast when expressed at high levels (Haubruck et al, 1987(Haubruck et al, , 1989. Subsequent studies have confirmed the role of RablA in ER-to-Golgi transport in mammalian tissue-culture cells (Nuoffer et al, 1994).…”

Molecular structure of ras‐related small GTP‐binding protein genes of rice plants and GTPase activities of gene products in Escherichia coli

“…By random sequencing of these cDNA libraries and computer anal- Fig. 2A shows a comparison of deduced amino acid sequences of ricl, yptm2 [lo], ara5 [6] and yptl [19]. ricl shares 83%, 81%, and 71% amino acid identity with yptm2, ara5 and yptl, respectively.…”

“…Fig. 2B shows a comparison of deduced amino acid sequences of ric2, ara2 [6], ypt3 [19] and rgpl [I 13. ric2 shares 79%, 68% and 58% amino acid identity with ara2, ypt3 and rgpl, respectively. The amino acid sequences of ric2 are conserved in four regions (shaded boxes in Fig.…”

“…Recently, many ras-related small GTP-binding protein genes were identified in various organisms including plants [6-l 21. To analyze an evolutional relationship between ricl and other ras-related small GTP-binding protein genes [3,6,11,19,23,24] an evolutional tree on the basis of amino acid homology was made (Fig. 3).…”