The Rate of Synthesis of Glycosaminoglycans and Collagen by Fibroblasts Cultured from Adult Human Liver Biopsies

Galambos, John T.; Hollingsworth, Martin A.; Falek, Arthur; Wd, Warren; McCain, Jack

doi:10.1172/jci108746

Cited by 58 publications

(17 citation statements)

References 18 publications

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“…Studies 25-30 years ago characterized the growth of mesenchymal cells derived from liver tissue, which in retrospect may have been derived from stellate cells (182,672). Cell studies of this type described a "fibroblast-like" phenotype with potential for extracellular matrix production.…”

Section: A Cell Isolation Methodsmentioning

confidence: 99%

“…Lower yields and dependence on costly technology greatly limit its applicability, although it remains valuable for obtaining ultrapurified stellate cells. Outgrowth of activated stellate cells from human liver biopsy material has gained favor (60, 691) and is reminiscent of earlier studies (182,672), albeit now utilizing more refined cell markers. The limitations of this approach are the potential heterogeneity of the culture and the inability to track early events in cellular activation, since the method relies on outgrowth of activated cells on plastic in tissue culture.…”

Section: A Cell Isolation Methodsmentioning

confidence: 99%

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Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver

Friedman

2008

Physiological Reviews

2,424

2,549

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The hepatic stellate cell has surprised and engaged physiologists, pathologists, and hepatologists for over 130 years, yet clear evidence of its role in hepatic injury and fibrosis only emerged following the refinement of methods for its isolation and characterization. The paradigm in liver injury of activation of quiescent vitamin A-rich stellate cells into proliferative, contractile, and fibrogenic myofibroblasts has launched an era of astonishing progress in understanding the mechanistic basis of hepatic fibrosis progression and regression. But this simple paradigm has now yielded to a remarkably broad appreciation of the cell's functions not only in liver injury, but also in hepatic development, regeneration, xenobiotic responses, intermediary metabolism, and immunoregulation. Among the most exciting prospects is that stellate cells are essential for hepatic progenitor cell amplification and differentiation. Equally intriguing is the remarkable plasticity of stellate cells, not only in their variable intermediate filament phenotype, but also in their functions. Stellate cells can be viewed as the nexus in a complex sinusoidal milieu that requires tightly regulated autocrine and paracrine cross-talk, rapid responses to evolving extracellular matrix content, and exquisite responsiveness to the metabolic needs imposed by liver growth and repair. Moreover, roles vital to systemic homeostasis include their storage and mobilization of retinoids, their emerging capacity for antigen presentation and induction of tolerance, as well as their emerging relationship to bone marrow-derived cells. As interest in this cell type intensifies, more surprises and mysteries are sure to unfold that will ultimately benefit our understanding of liver physiology and the diagnosis and treatment of liver disease.

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Section: A Cell Isolation Methodsmentioning

confidence: 99%

Section: A Cell Isolation Methodsmentioning

confidence: 99%

Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver

Friedman

2008

Physiological Reviews

2,424

2,549

View full text Add to dashboard Cite

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“…Culture and characterization of myofibroblasts grown from human liver explants of normal and fibrotic livers were reported about 40 years ago [35][36][37]. Tissue fragments were attached to plastic substratum.…”

Section: Liver Tissue Explantsmentioning

confidence: 99%

“…The outgrowth of cells with myofibroblast characteristics became detectable after 10-15 days, and the myofibroblastic cells were recovered and passaged by trypsinization after 3-4 weeks culturing from the explants of liver sections [38]. Generally, studies were carried out on homogeneous cultures of ''smooth muscle cells'' subcultured between three and ten passages, without noticeable phenotypic alterations and significant result variation from various passages [36,[38][39][40][41].…”

Section: Liver Tissue Explantsmentioning

confidence: 99%

“…Under these culture conditions, two cell types, which resemble smooth muscle cells and vascular endothelium, grew from the liver tissue [37], while epithelial cells were no longer present in these subcultures [36,37]. Moreover, using this culture method, early events of HSC activation cannot be traced and investigated, since it takes weeks for the cells to grow out of the liver tissue and onto plastic surface.…”

Section: Liver Tissue Explantsmentioning

confidence: 99%

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Human hepatic stellate cell isolation and characterization

et al. 2017

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The hepatic stellate cells (HSCs) localize at the space of Disse in the liver and have multiple functions. They are identified as the major contributor to hepatic fibrosis. Significant understanding of HSCs has been achieved using rodent models and isolated murine HSCs; as well as investigating human liver tissues and human HSCs. There is growing interest and need of translating rodent study findings to human HSCs and human liver diseases. However, species-related differences impose challenges on the translational research. In this review, we focus on the current information on human HSCs isolation methods, human HSCs markers, and established human HSC cell lines.

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Perivenular Fibrosis as Precursor Lesion of Cirrhosis

Worner¹,

Lieber²

1985

JAMA

158

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Thirty-four male alcoholics underwent sequential liver biopsies as part of their evaluation. Of 19 subjects with simple fatty livers, only three showed progression of liver disease: one developed perivenular fibrosis after two years; a second showed no progression after three years, but developed perivenular fibrosis after four years; the third subject likewise showed no progression after one year, but developed incomplete cirrhosis after six years. In contrast, of 15 subjects with perivenular fibrosis at the time of the initial biopsy, 13 progressed to more severe stages of liver disease during a one- to four-year follow-up interval. Nine developed fibrosis, one developed incomplete cirrhosis and three developed cirrhosis. Thus, patients with perivenular fibrosis at the fatty liver stage are likely to progress to more severe stages of alcoholic liver disease if they continue to consume alcohol.

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The Rate of Synthesis of Glycosaminoglycans and Collagen by Fibroblasts Cultured from Adult Human Liver Biopsies

Cited by 58 publications

References 18 publications

Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver

Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver

Human hepatic stellate cell isolation and characterization

Perivenular Fibrosis as Precursor Lesion of Cirrhosis

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