The ratio of ADSCs to HSC‐progenitors in adipose tissue derived SVF may provide the key to predict the outcome of stem‐cell therapy

Kilinc, Mehmet O.; Santidrián, Antonio F.; Minev, Ivelina; Toth, R. A.; Nguyen, Duong Thanh; Lander, Elliot B.; Berman, Mark; Minev, Boris; Szalay, Aladar A.

doi:10.1186/s40169-018-0183-8

Cited by 42 publications

(44 citation statements)

References 55 publications

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“…The SVF fraction can be roughly divided into CD45 + cells, which contain the leukocytes, and CD45cells, comprising the ASCs, ECs, EPCs, etc. Although previous studies have indicated the efficacy of leukocytes in SVF employed in clinical treatments [Le et al, 2018], the focus of most clinical research has been to characterize the ASC-containing CD45cell fraction [Kilinc et al, 2018]. The live ASC cell population in the SVF was significantly reduced over time and declined with increasing temperatures, and the most prominent decrease was observed during storage at 37 ° C. Here, we report that storage significantly alters the SVF cell composition.…”

Section: Figure 2 Monocytesmentioning

confidence: 75%

Lipoaspirate Storage Time and Temperature: Effects on Stromal Vascular Fraction Quality and Cell Composition

Svalgaard

Juul

Vester-Glovinski

et al. 2020

Cells Tissues Organs

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The adipose tissue-derived stromal vascular fraction (SVF) is a promising candidate for use in cell therapy and tissue engineering due to its regenerative and immunomodulatory properties. Some therapies are based on using the complete SVF product, whereas others depend on the expansion of adipose-derived stromal cells (ASCs) in culture. The latter application often involves a time delay between adipose tissue harvest and SVF isolation. This study investigated how storage time and temperature affected cell quality and composition. Aliquots of lipoaspirate were stored cold (4 ° C), at room temperature (18-20 ° C), or at 37 ° C. SVF was isolated on sequential time points over a period of 48 h, and the following were assessed: cell viability, vitality, composition, and the proliferative potential of the ASCs. When the lipoaspirate was stored cold, the viability of the SVF remained stable for up to 48 h; however, the vitality of the SVF decreased significantly after 24 h. When stored at higher temperatures (room temperature or 37 ° C), the vitality of the SVF decreased after 8 h. The ASC fraction in the SVF decreased rapidly after 8 h when stored at higher temperatures, whereas this change was delayed significantly when the lipoaspirate was stored cold. Tendencies towards increases in the lag phase, population doubling time (PDt), and time to reach confluency were observed when the lipoaspirate was stored at higher temperatures. The vitality of the SVF was correlated significantly with the time of the lag phase and the time required to reach confluence, whereas no correlation was observed with the PDt. Both prolonged storage time and increased temperature during lipoaspirate storage negatively affected the quality of the obtained SVF. Our results suggest that lipoaspirate should be stored for no longer than 24 h at 4 ° C to maintain the optimal quality for the isolation of SVF and the expansion of ASCs.

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Section: Figure 2 Monocytesmentioning

confidence: 75%

Lipoaspirate Storage Time and Temperature: Effects on Stromal Vascular Fraction Quality and Cell Composition

Svalgaard

Juul

Vester-Glovinski

et al. 2020

Cells Tissues Organs

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“…ASCs attracted significantly more Lineage − Sca-1 + c-kit + cells to the BM in comparison with BM-MSCs. This supportive effect in hematopoietic recovery was a result of the dose-dependent effect of ASCs [31], but the effective ASC:HSC ratio differed among several research groups [31,32]. ASCs were also able to help hematopoietic reconstitution when they were directly The number of registered clinical trials is obtained from the website of the U.S. National Library of Medicine under the terms "adipose-derived stromal/stem cells" and "bone marrow mesenchymal stromal/stem cells" respectively (until September 2019)…”

Section: Hematopoiesis-regulating Properties Of Ascsmentioning

confidence: 99%

Adipose-derived stromal cells in regulation of hematopoiesis

et al. 2020

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Over the past decade, mesenchymal stromal cells (MSCs) found in the bone marrow microenvironment have been considered to be important candidates in cellular therapy. However, the application of MSCs in clinical settings is limited by the difficulty and low efficiency associated with the separation of MSCs from the bone marrow. Therefore, distinct sources of MSCs have been extensively explored. Adipose-derived stromal cells (ASCs), a cell line similar to MSCs, have been identified as a promising source. ASCs have become increasingly popular in many fields, as they can be conveniently extracted from fat tissue. This review focuses on the properties of ASCs in hematopoietic regulation and the underlying mechanisms, as well as the current applications and future perspectives in ASC-based therapy.

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“…Patients received intraventricular SVF injection volumes of 3.5-20 cc (median: 4 cc) containing 4.05 × 10 5 to 6.2 × 10 7 cells/cc and contained on average 8% hematopoietic and 7.5% ADSCs by fluorescence-activated cell sorting (FACS) cytometry [11,45].…”

Section: Patientsmentioning

confidence: 99%

Human intracerebroventricular (ICV) injection of autologous, non-engineered, adipose-derived stromal vascular fraction (ADSVF) for neurodegenerative disorders: results of a 3-year phase 1 study of 113 injections in 31 patients

Duma¹,

Kopyov²,

Kopyov³

et al. 2019

Mol Biol Rep

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We have chosen to test the safety of human intracerebroventricular (ICV) brain injections of autologous non-geneticallymodified adipose-derived stromal vascular fraction (ADSVF). In this IRB-approved trial, 24 patients received ICV ADSVF via an implanted reservoir between 5/22/14 and 5/22/17. Seven others were injected via their ventriculo-peritoneal shunts. Ten patients had Alzheimer's disease (AD), 6 had amyotrophic lateral sclerosis (ALS), 6 had progressive multiple sclerosis (MS-P), 6 had Parkinson's "Plus" (PD+), 1 had spinal cord injury, 1 had traumatic brain injury, and 1 had stroke. Median age was 74 (range 41-83). Injections were planned every 2-3 months. Thirty-one patients had 113 injections. Patients received SVF injection volumes of 3.5-20 cc (median:4 cc) containing 4.05 × 10 5 to 6.2 × 10 7 cells/cc, which contained an average of 8% hematopoietic and 7.5% adipose stem cells. Follow-up ranged from 0 to 36 months (median: 9.2 months). MRIs post injection(s) were unchanged, except for one AD patient whose hippocampal volume increased from < 5th percentile to 48th percentile (NeuroQuant ® volumetric MRI). Of the 10 AD patients, 8 were stable or improved in tests of cognition. Two showed improvement in P-tau and ß-amyloid levels. Of the 6 MS-P patients all are stable or improved. Four of 6 ALS patients died of disease progression. Twelve of 111 injections (11%) led to 1-4 days of transient meningismus, and mild temperature elevation, which resolved with acetaminophen and/or dexamethasone. Two (1.8% of injections) required hospitalization for these symptoms. One patient (0.9% of injections) had his reservoir removed and later replaced for presumed infection. In this Phase 1 safety trial, ADSVF was safely injected into the human brain ventricular system in patients with no other treatment options. Secondary endpoints of clinical improvement or stability were particularly promising in the AD and MS-P groups. These results will be submitted for a Phase 2 FDA-approved trial.

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The ratio of ADSCs to HSC‐progenitors in adipose tissue derived SVF may provide the key to predict the outcome of stem‐cell therapy

Cited by 42 publications

References 55 publications

Lipoaspirate Storage Time and Temperature: Effects on Stromal Vascular Fraction Quality and Cell Composition

Lipoaspirate Storage Time and Temperature: Effects on Stromal Vascular Fraction Quality and Cell Composition

Adipose-derived stromal cells in regulation of hematopoiesis

Human intracerebroventricular (ICV) injection of autologous, non-engineered, adipose-derived stromal vascular fraction (ADSVF) for neurodegenerative disorders: results of a 3-year phase 1 study of 113 injections in 31 patients

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