The reactive extraction of phenylalanine with aliquat 336: Buffer co-extraction equilibrium and mass transfer kinetics

Scarpello, Justin T.; Stuckey, David C.

doi:10.1002/1097-0290(20000905)69:5<469::aid-bit1>3.0.co;2-m

Cited by 15 publications

(9 citation statements)

References 20 publications

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“…Scheme of the mechanism assumed for the extraction of GlcNAc, ManNAc and Neu5Ac. by ion transfer was subject to several publications since the 1980s [34][35][36][37][38][39][40]. The kinetic model used here to describe the reactive twostep extraction of the carbohydrates and pyruvate is based on a model published by Chan and Wang [41] for the reactive extraction of amino acids.…”

Section: Theorymentioning

confidence: 99%

Reactive extraction of N-acetylneuraminic acid—Kinetic model and simulation of integrated product removal

Zimmermann

Masuck

Kragl

2008

Separation and Purification Technology

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Section: Theorymentioning

confidence: 99%

Reactive extraction of N-acetylneuraminic acid—Kinetic model and simulation of integrated product removal

Zimmermann

Masuck

Kragl

2008

Separation and Purification Technology

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“…The previous models were developed for a contactor of similar design to the Lewis cell used in this present study where a twofilm theory model of interphase mass transfer was assumed. In addition to the standard assumptions of twofilm theory (i.e., steady-state transfer and equilibrium at the interface), it is assumed that for reactive extraction using Aliquat 336 the extraction reaction rate is instantaneous and does not limit the mass transfer process (2)(3)(4)(5)11). Figure 1 shows a schematic of the concentration profiles either side of the interface according to the twofilm theory for the case under study.…”

Section: Theorymentioning

confidence: 99%

“…A suitable extraction system involving the recovery of phenylalanine, an amino acid, by the quaternary ammonium compound Aliquat 336 has been studied previously by a number of researchers with recovery yields of above 95% being reported (2)(3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

Co‐Extraction during Reactive Extraction of Phenylalanine using Aliquat 336: Interfacial Mass Transfer

Pursell¹,

Mendes-Tatsis²,

Stuckey³

2003

Biotechnology Progress

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Reactive liquid-liquid extraction can be used to separate hydrophilic fermentation products that would not otherwise partition into nonpolar solvents. However, during extraction of the target solute other compounds present in the extraction medium will also react with the ion exchange reagent and are thus co-extracted. In this study the effect of co-extraction on the interfacial flux of the target solute phenylalanine has been investigated for reactive extraction using Aliquat 336. The effect of co-extracting compounds has been included in a new interfacial flux balance, and experimental results reveal that the interfacial concentrations are equal to the final equilibrium conditions of the system. Using this information a simple mass transfer model has been developed from which film mass transfer coefficients may be determined. Co-extraction of other compounds present in the feed was found to reduce the interfacial flux of the target solute by reducing the driving force. Co-extraction did not affect the value of the film mass transfer coefficient, and therefore, co-extraction does not effect the transport properties of the solute to the interface. Extraction from a multicomponent fermentation broth resulted in a reduced flux, which arises from a reduction in the driving force caused by high levels of co-extraction. Furthermore, the flux was also reduced as the result of a mass transfer resistance caused by soluble surface-active compounds present in the fermentation broth adsorbing to the interface. The biomass associated with the fermentation broth was also found to reduce the solute flux, and it is believed that this is due to blockage of the interfacial area.

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“…In addition to separation approaches for organic acids, the applicability of reactive extraction for amino acid separation was studied previously [17,18,19,20,21,22,23,24,25], also focusing on L-Phe separation [26] and aiming at a detailed description of L-Phe mass-transfer kinetics [8,27,28,29]. However, most of these studies used ''aqueous model solutions'' instead of complex fermentation media and focused their interest only on anionselective carriers like Aliquat 336.…”

Section: List Of Symbolsmentioning

confidence: 99%

“…The quarternary ammonia salt trioctyl-methyl ammonium chloride (Aliquat 336, Henkel, Germany) represents a standard liquid anion exchanger for the extraction of heavy metals on an industrial scale but also for enzymes [30], organic and amino acids [8,9,10,11,12,13,14,15,16,27,28,29]. Furthermore, tri-isooctyl-amine (Alamine 308, Henkel, Germany) was considered.…”

Section: Anion-specific Carriersmentioning

confidence: 99%

Integrated L-phenylalanine separation in an E. coli fed-batch process: from laboratory to pilot scale

Maass

et al. 2002

Bioprocess and Biosystems Engineering

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Pilot-scale reactive-extraction technology for fully integrated L-phenylalanine (L-Phe) separation in Escherichia coli fed-batch fermentations was investigated in order to prevent an inhibition of microbial L-Phe production by-product accumulation. An optimal reactive-extraction system, consisting of an organic kerosene phase with the cation-selective carrier DEHPA (di-2-ethylhexyl phosphonic acid) and an aqueous stripping phase including sulphuric acid, was found particularly efficient. Using this system with two membrane contactors, mass-transfer coefficients of up to 288 x 10(-7) cm s(-1) for the aqueous/organic and 77 x 10(-7) cm s(-1) for the organic/stripping phase were derived from experimental data using a simple modelling approach. Concentration factors higher than 4 were achieved in the stripping phase as compared to the aqueous donor phase. Reactive extraction enabled a 98% cation portion of L-Phe in the stripping phase, leading to final product purity higher than 99% after L-Phe precipitation. A doubling of L-Phe/glucose yield was observed when kerosene/DEHPA was added to the fermentation solution in the bioreactor to experimentally simulate a fully integrated L-Phe separation process.

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The reactive extraction of phenylalanine with aliquat 336: Buffer co-extraction equilibrium and mass transfer kinetics

Cited by 15 publications

References 20 publications

Reactive extraction of N-acetylneuraminic acid—Kinetic model and simulation of integrated product removal

Reactive extraction of N-acetylneuraminic acid—Kinetic model and simulation of integrated product removal

Co‐Extraction during Reactive Extraction of Phenylalanine using Aliquat 336: Interfacial Mass Transfer

Integrated L-phenylalanine separation in an E. coli fed-batch process: from laboratory to pilot scale

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