The real potential of semen production of Bali Bull: over year observation at Singosari National Artificial Insemination Center (SNAIC), Singosari-Indonesia

Nugraha, Chairdin Dwi; Widodo, Nashi; Kuswati, Kuswati; Suyadi, Suyadi

doi:10.1051/e3sconf/202233500045

Cited by 4 publications

(5 citation statements)

References 12 publications

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“…The relationship between testosterone with sperm quality and sperm motility has not been proven (Rajak et al 2014a;Rajak et al 2014b). Bali cattle producing frozen semen's productivity is essential and closely related to the value of semen volume, sperm motility, and sperm concentration (Nugraha et al 2022). Information regarding the correlation of semen quality, libido, and testosterone concentration in Bali bulls is limited.…”

Section: Introductionmentioning

confidence: 99%

Correlation between Semen Quality, Libido, and Testosterone Concentration in Bali Bulls

Iskandar

Sonjaya²,

Arifiantini

et al. 2022

JITV

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Indonesia has two National Artificial Insemination Centers (AIC) and more than 15 Regional Artificial Insemination Centers (RAIC) spread across several provinces. Bulls in the AIC must have a high libido and produce good quality semen. This study examines the correlation between libido with semen quality and testosterone concentration to determine potential frozen semen production from Bali bulls in South Sulawesi RAIC. Ten Bali bull were used in this study. Semen collection was carried out twice a week with semen evaluation following the RAIC protocol. At the same time, blood samples and libido measurements were carried out from each bull. The frozen semen production potential was calculated by multiplying the semen volume, motility, and sperm concentration. The results showed that the quality of fresh semen and testosterone concentrations did not differ between high and low libido of Bali bulls. Libido has a positive correlation with semen volume (r= 0.52) and sperm motility (r= 0.62), while testosterone concentration has a negative correlation with semen volume (r= -0.65), sperm motility (r= -0.60), and libido (r= -0.48). Bulls with high and low libido have good frozen semen production potential, ranging from 19,755 – 21,640 straws per year. Bali bulls in RAIC have fresh semen quality and testosterone concentrations under normal conditions, with high potential for frozen semen production, although only 60% of Bali cattle have high libido and 40% have low libido.

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Section: Introductionmentioning

confidence: 99%

Correlation between Semen Quality, Libido, and Testosterone Concentration in Bali Bulls

Iskandar

Sonjaya²,

Arifiantini

et al. 2022

JITV

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“…The highest ejaculate volume corresponded to 4 and 5 years-old bulls, but the peak volume can be achieved at different ages in various breeds [ 15 ]. Bali bull semen volume can vary due to age, climate, temperature, and cage humidity [ 4 ]. The color of the semen obtained in this study was milky white, similar to that of normal cattle.…”

Section: Discussionmentioning

confidence: 99%

“…To achieve the sustainable breeding of Bali bulls, good re productive performance of both female and male cattle is important to achieve genetic improvement, particularly when driven by artificial insemination (AI) programs [ 4 ]. AI has been effectively used in livestock species like cattle, sheep, and goats.…”

Section: Introductionmentioning

confidence: 99%

Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

et al. 2023

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Background:The conservation of Bali bulls, the Indonesian native breed of cattle, is crucial for cattle breeding in Indonesia. To guarantee the spread of Bali bulls through artificial insemination the quality of the frozen semen must be high. To this end, using an extender material in addition to semen increasing spermatozoa's survival during cryopreservation is important. Green tea extract (GTE) can be used as cryoprotectant because its high antioxidant activity can help avoid reactive oxygen species formation.Methods: Semen of five Bali bulls from the National Artificial Insemination Center at Singosari, Indonesia was collected routinely twice a week. First, fresh semen inspection was performed to determine the feasibility of using Bali bulls as animal samples. The extender used in this study was Tris-based egg yolk. The divided samples into four treatments: T0 (no GTE added to the extender), T1 (0.05 mg GTE plus 100 mL extender), T2 (0.10 mg GTE plus 100 mL extender), and T3 (0.15 mg GTE plus 100 mL extender). The semen freezing process was conducted according to standard procedures and sperm quality parameters, i.e., sperm motility, viability, abnormalities, and membrane integrity observed pre-freezing and postthawing.Results: There were significant differences in total motility, progressive motility, viability, and integrity membrane of Bali bull sperm at both pre-freezing and post-thawing after adding GTE into the extender. In contrast, there were no differences in abnormalities among treatments. Conclusion:Adding GTE at a 0.15 mg into 100 mL Tris-based egg yolk extender can improve the quality of cryopreserved Bali bull sperm.

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“…Sperm DNA testing using the Sperm-Bos-Halomax® method determined the sperm DNA fragmentation index (SDFi) (Baiee et al 2018) (Figure 1). The evaluation of sperm DNA damage is important for revealing infertility in males and examining motility and viability (Nugraha et al 2022). Damage to the sperm's DNA can lead to embryonic death.…”

Section: Evaluation Of Spermatozoa Dna Fragmentationmentioning

confidence: 99%

Evaluation Deoxyribonucleic acid (DNA) fragmentation of local Indonesian cattle frozen sperm using Halomax method

et al. 2023

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Abstract. Prabowo TA, Bintara S, Yusiati LM, Sitaresmi PI, Widayati. 2023. Evaluation Deoxyribonucleic acid (DNA) fragmentation of local Indonesian cattle frozen sperm using Halomax method. Biodiversitas 24: 2225-2230. Deoxyribonucleic acid (DNA) is a molecular chain containing genetic material that determines the quality of each individual produced. DNA damage in cattle can affect fertility and miscarriage of embryos. There is no simple research on DNA damage in local Indonesian cattle (Bos indicus Linnaeus 1758); therefore, miscarriage and infertility cases in Indonesia are still high, one of which can be attributed to sperm DNA damage. This study aimed to evaluate DNA damage in the frozen semen of local Indonesian cattle and suggested SDF (Sperm DNA Fragmentation) inclusion screening as the main requirement for frozen semen. The materials used in this study were 54 straws of frozen semen from nine males from three different types of local cattle, including Bali, Madura, and Aceh. Each type consisted of three bulls, each with six replicates. Sperm-Bos-Halomax was used to examine sperm DNA damage. Data were analyzed using one-way analysis of variance (ANOVA). Aceh cattle had the highest post average compared to Bali and Madura at 63.33±2.43%. Abnormality of Balinese cattle had the lowest average compared to Aceh and Madura at 8.83±1.04%. Evaluation of sperm DNA damage in local cattle using the Sperm-Bos-Halomax® method showed that the highest average DNA damage was 1.72±0.62% in Aceh, and the lowest was 1.67±0.66% in Madura cattle. The sperm DNA damage in Bali cattle was 1.67±0.70%. The sperm DNA quality in the frozen semen of the local cattle in this study was good, but it is still necessary to determine SDF as the main standard in frozen semen.

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The real potential of semen production of Bali Bull: over year observation at Singosari National Artificial Insemination Center (SNAIC), Singosari-Indonesia

Cited by 4 publications

References 12 publications

Correlation between Semen Quality, Libido, and Testosterone Concentration in Bali Bulls

Correlation between Semen Quality, Libido, and Testosterone Concentration in Bali Bulls

Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

Evaluation Deoxyribonucleic acid (DNA) fragmentation of local Indonesian cattle frozen sperm using Halomax method

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