The recovery of DNA profiles from saliva and touch evidences after postal bomb explosion

Berti, Andrea; Barni, F.; Virgili, Amedeo; Colozza, C.; Maiorino, F.; Tocca, M.

doi:10.1016/j.fsigss.2011.09.097

Cited by 11 publications

(8 citation statements)

References 1 publication

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“…These type of analyses are often associated with artefacts: allele drop-in or drop-out, heterozygote peak imbalance and elevated stutter frequencies 18 , resulting in fluctuating results throughout different analyses. Thermal degradation caused by exposure to high temperatures, resulting in cleavage of covalent bonds within DNA strands, has been observed earlier but did not preclude DNA profiling post-blast depending on the type and amount of explosive 19 .…”

Section: Introductionmentioning

confidence: 63%

“…A first limitation of the current study is the use of a low amount of C-4 (7 g) compared to real-life cases in order of magnitude in which terrorists often use a larger amount of explosive. This difference has an effect on the temperature developed during the blast 19 and subsequently on the possibility of recovering FPs and DNA from the IED components 18 , 27 , 28 , as well as on the fragmentation of the IED itself 29 . A higher amount of explosive implies a more challenging recovery of FPs and potential DNA carrier components.…”

Section: Discussionmentioning

confidence: 99%

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Survival of forensic trace evidence on improvised explosive devices: perspectives on individualisation

Vanderheyden

Verhoeven²,

Vermeulen³

et al. 2020

Sci Rep

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Neutralisation of improvised explosive devices. A ballistic water jet was fired onto the suitcases with a RE70 M3 Plus Waterjet Disruptor (Chemring Technology Solutions). This weapon interrupts electrical circuits without recoil by firing an electrically initiated 0.5 caliber cartridge. This cartridge contains a projected waterjet of 150 ml, fired under high pressure. The weapon was oriented 45 • with the longitudinal surface of the suitcase. After each disarmament, fragments were collected and packaged separately. A test setup of this experiment can be found in Supplementary Figure S2. Detonation of improvised explosive devices. Prior to the experiment, a safety perimeter of 200 m was set. A measured amount of C-4 (7 g) was placed in the metal cans and the suitcases were closed with a zipper. The ignition was electrically initiated from a distance. The presence of wooden crates around each side of the test setup prevented the spread of post-explo fragments. Relocation of the test setup after every explosion prevented cross-contamination. After each detonation, fragments were collected and packaged separately. The test setup of this experiment can be found in Supplementary Figure S3.

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Section: Introductionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Survival of forensic trace evidence on improvised explosive devices: perspectives on individualisation

Vanderheyden

Verhoeven²,

Vermeulen³

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…However, Gashi et al measured the temperatures reached by the exhaust gases which were found to be significantly higher than experienced cartridge casings 15 [31][32][33][34] . The high pressure high heat environments caused by these explosions are similar in nature to those created for the combustion of smokeless powders and in some cases IEDs use large amount of smokeless powders as their accelerants 31 .…”

Section: Due To Heatmentioning

confidence: 97%

DNA recovery from fired hollow point ammunition

Booth

Chapman

2019

Australian Journal of Forensic Sciences

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Crime involving firearms are a concern to police departments worldwide. In the event that a firearm is used, a projectile and a cartridge case are likely to be expelled. Current methods for identification of cartridges and bullets from crime scenes involve the use of comparison microscopes for class and individual characteristics generated by the firearm. With the use of recent genetic profiling technology, identifying persons that have come into contact with ammunition before and after firing has become a possibility. Results for identification of those who have handled cartridge cases through DNA profiling has been found to be consistently reliable in controlled laboratory settings if appropriate and thorough recovery techniques are utilised. DNA from cartridges, casings and bullets from real life crime scenes have also been found to provide reproducible and reliable profiles in a quarter of the exhibits that were involved in the study. Due to the nature of this study, the environments were not controlled, and understanding of the downfalls were not fully understood. Furthermore, little analysis has been conducted regarding recovery of DNA from the projectile rather than the cartridge. This review aims to establish the factors that may influence the recovery of DNA from fired ammunition. These include the loading of the ammunition, the temperatures and pressure involved in the combustion process, the by-products of the combustion process and methodology used to remove DNA from the bullet. This discussion will aid in the resulting methodology for use in a pilot study, as proof of concept in this area has yet to be conducted.

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“…Microfluidic systems have been modified to integrate steps, including the use of an on-chip extraction paired with a multiplexed STR amplification [92]. Even in cases where improvised explosive disposals (IEDs) have thought to destroy most DNA evidence or limit the amount of DNA that can be extracted, improved multiplexes have been able to identify STR profiles [93].…”

Section: Microfluidic Systems For Dna Analysismentioning

confidence: 99%

Recent Advances in Forensic DNA Analysis

Romeika

Yan²

2014

J Forensic Res

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Forensic DNA (deoxyribonucleic acid) analysis or DNA profiling has played a major role in the criminal justice system. New techniques and technologies for DNA profiling continue to evolve every year. This paper reviews the literature reported during January 2011 through June 2013 in the field of forensic DNA analysis. Recent advances in almost all aspects of DNA analysis-which include sample collection, storage, and pretreatment, DNA extraction, DNA quantitation, quality assurance of DNA testing, and DNA databases are discussed. New developments now enable new kinds of forensically relevant information to be extracted from limited quantities of biological samples, and offer unprecedented potential for high-throughput DNA analysis.

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The recovery of DNA profiles from saliva and touch evidences after postal bomb explosion

Cited by 11 publications

References 1 publication

Survival of forensic trace evidence on improvised explosive devices: perspectives on individualisation

Survival of forensic trace evidence on improvised explosive devices: perspectives on individualisation

DNA recovery from fired hollow point ammunition

Recent Advances in Forensic DNA Analysis

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