The redox state of the plastoquinone pool directly modulates minimum chlorophyll fluorescence yield in <i>Chlamydomonas reinhardtii</i>

Hohmann‐Marriott, Martin F.; Takizawa, Kayoko; Eaton‐Rye, Julian J.; Mets, Laurens; Minagawa, Jun

doi:10.1016/j.febslet.2010.01.052

Cited by 21 publications

(15 citation statements)

References 53 publications

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“…Ultimately, this results in a drop in the maximal fluorescence yield in state 2 conditions ( Figure 1A; see Supplemental Figures 1A and 1C online), which was present in the two strains containing an active kinase (the wild type and npq4) but absent in stt7-9 (as expected because of the disruption of STT7) and in the five npq4 stt7-9 clones. In these strains, a small fluorescence increase was observed in state 2 conditions, a phenomenon already observed upon reduction of the PQ pool in strains impaired in state transitions (Hohmann-Marriott et al, 2010).…”

Section: Generation Of a Double Mutant Impaired In State Transitions mentioning

confidence: 96%

A Dual Strategy to Cope with High Light in Chlamydomonas reinhardtii

et al. 2013

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Absorption of light in excess of the capacity for photosynthetic electron transport is damaging to photosynthetic organisms. Several mechanisms exist to avoid photodamage, which are collectively referred to as nonphotochemical quenching. This term comprises at least two major processes. State transitions (qT) represent changes in the relative antenna sizes of photosystems II and I. High energy quenching (qE) is the increased thermal dissipation of light energy triggered by lumen acidification. To investigate the respective roles of qE and qT in photoprotection, a mutant (npq4 stt7-9) was generated in Chlamydomonas reinhardtii by crossing the state transition-deficient mutant (stt7-9) with a strain having a largely reduced qE capacity (npq4). The comparative phenotypic analysis of the wild type, single mutants, and double mutants reveals that both state transitions and qE are induced by high light. Moreover, the double mutant exhibits an increased photosensitivity with respect to the single mutants and the wild type. Therefore, we suggest that besides qE, state transitions also play a photoprotective role during high light acclimation of the cells, most likely by decreasing hydrogen peroxide production. These results are discussed in terms of the relative photoprotective benefit related to thermal dissipation of excess light and/ or to the physical displacement of antennas from photosystem II.

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Section: Generation Of a Double Mutant Impaired In State Transitions mentioning

confidence: 96%

A Dual Strategy to Cope with High Light in Chlamydomonas reinhardtii

et al. 2013

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“…Steady-state fluorescence values (F s ) in the light are indicative of an over-reduced plastoquinone pool and consistent with diminished PS I function (32). Fig.…”

Section: Ppd1 Rnai Individuals Exhibit a Range Of Phenotypes Formentioning

confidence: 99%

The PsbP Domain Protein 1 Functions in the Assembly of Lumenal Domains in Photosystem I

Roose

Frankel

Bricker

2014

Journal of Biological Chemistry

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“…When cells are spun down for washing with the fresh medium, it is crucial to develop a rapid procedure to do so, as prolonged exposure to new conditions, such as the absence of light within the centrifuge or changes in pH may elicit a physiological response. A physiological response that has severe consequences for Chl fluorescence within a short period is anaerobic incubation (Hohmann-Marriott et al 2010) that can rapidly occur within cell pellets.…”

Section: Cell Suspensions and Thylakoidsmentioning

confidence: 99%

Chlorophyll fluorescence emission spectroscopy of oxygenic organisms at 77 K

Lamb¹,

Røkke²,

Hohmann‐Marriott³

2018

Photosynt.

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Photosynthetic fluorescence emission spectra measurement at the temperature of 77 K (-196°C) is an often-used technique in photosynthesis research. At low temperature, biochemical and physiological processes that modulate fluorescence are mostly abolished, and the fluorescence emission of both PSI and PSII become easily distinguishable. Here we briefly review the history of low-temperature chlorophyll fluorescence methods and the characteristics of the acquired emission spectra in oxygen-producing organisms. We discuss the contribution of different photosynthetic complexes and physiological processes to fluorescence emission at 77 K in cyanobacteria, green algae, heterokont algae, and plants. Furthermore, we describe practical aspects for obtaining and presenting 77 K fluorescence spectra.

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The redox state of the plastoquinone pool directly modulates minimum chlorophyll fluorescence yield in Chlamydomonas reinhardtii

Cited by 21 publications

References 53 publications

A Dual Strategy to Cope with High Light in Chlamydomonas reinhardtii

A Dual Strategy to Cope with High Light in Chlamydomonas reinhardtii

The PsbP Domain Protein 1 Functions in the Assembly of Lumenal Domains in Photosystem I

Chlorophyll fluorescence emission spectroscopy of oxygenic organisms at 77 K

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