The Regeneration of Plants Free of LSV and TBV From Infected Lilium Bulb-Scale Explants in the Presence of Virazole

Blom-Barnhoorn, G.J.; Aartrijk, J. van

doi:10.17660/actahortic.1985.164.17

Cited by 11 publications

(12 citation statements)

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“…Tissue culture companies can play an important role in the project, as the application of meristem culture can keep lilies free from LSV (Asjes et al 1974;Van Aartrijk & Blom-Barnhoorn, 1978), and TBV (Blom- Barnhoorn & Van Aartrijk, 1985). LVX does not frequently occur, so usually LVX-free starting material can be selected.…”

Section: Virus Damagementioning

confidence: 99%

Production of high quality, healthy ornamental crops through meristem culture

Zaayen¹,

Eijk²,

Versluijs³

1992

Acta Botanica Neerlandica

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An increasing number of ornamental crops are being multiplied in vitro. In this article, the application of meristern culture to plants free from viruses is reviewed and is shown to be useful and cost-effective. First, a literature survey is given on results ofmeristem culture applied to ornamentals and on damage due to virus diseases. Secondly trials and results with lily, Aistroemeria and Delphinium and their viruses are discussed.Virus-free plants have a considerable surplus value compared with infected plants. Additional advantages are that a virus-free crop cannot be a source of contamination for other varieties of the same crop, and that there are no phytosanitary limitations. Virus-free does not mean resistant, however, and re-infection is possible, but can be prevented by strict measures.Meristem culture applied to in vitro cultures oflily, Alstroemeria and Delphinium always yielded a much lower amount of virus-free plantlets than the usual procedure, i.e. meristem culture applied to plants grown in the glasshouse.

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Section: Virus Damagementioning

confidence: 99%

Production of high quality, healthy ornamental crops through meristem culture

Zaayen¹,

Eijk²,

Versluijs³

1992

Acta Botanica Neerlandica

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“…Adenine arabinoside at 10mg1-1 and higher, completely retarded the growth of the meristems. Simpkins et al (1981) and Blom-Barnhoorn & Van Aartrijk (1985) Table I. Average number of Ornithogalurn cv.…”

Section: The Effect Of Anti-viral Compoundsmentioning

confidence: 99%

“…Four different viruses were then eliminated from the plants. Likewise, Klein & Livingstone (1982) and Blom-Barnhoorn & Van Aartrijk (1985) found that the addition of Virazole (ribavirin) aided the elimination of viruses from potatoes and lilies respectively. There is, however, no known chemical method for totally eliminating viruses from bulbous plants while other treatments such as heat only seem to reduce the virus concentration (Bertaccini et al 1989).…”

Section: Introductionmentioning

confidence: 99%

“…These include: -exploiting erratic virus distribution within plants -meristem-tip culture -thermotherapy -chemotherapy A combination of these techniques is sometimes used. Since Morel & Martin (1952) first demonstrated the elimination of viruses from Dahlia plants by the use of apical meristems, other researchers also succeeded in eliminating viruses from bulbous ornamentals through meristem-tip culture, for example Freesia (Brants & Vermeulen 1965) Hyacinthus (Asjes et al 1974), Lilium (Blom-Barnhoorn & Van Aartrijk 1985) and Gladiolus (Bertaccini & Marani 1986). …”

Section: Introductionmentioning

confidence: 99%

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Elimination of ornithogalum mosaic virus in the Ornithogalum cv. Rogel through meristem tip culture and chemotherapy

Vcelar¹,

Ferreira²,

Niederwieser³

1992

Plant Cell Tiss Organ Cult

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Virus-indexed Ornithogalum cv. Rojel plants were produced by eliminating Ornithogalum mosaic virus (OMV) through meristem-tip culture. The best plantlet regeneration was obtained from meristems derived from adventitious buds which developed on leaf explants taken from mother plants at the flowering stage. Acyclovir had no effect as an anti-viral compound on plantlet regeneration or virus elimination. Adenine arabinoside retarded plantlet development at concentrations of 10mg1-1 and higher, while 5.0 mg 1 -I suppressed the virus concentration beneath a detectable level in young plants. All the mature plants, however, tested positively for the presence of OMV.

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“…The elimination of LSV was achieved in Lilium × elegans and L. longiflorum by tissue culture in early excision of shoots regenerated from scale pieces , meristematic tip culture Nesi et al, 2009), and meristematic tip (Nesi et al, 2009) or callus (Xu et al, 2000) culture in combination with thermotherapy. Meristematic tip culture was also used to eliminate Tulip breaking virus (TBV), which is genetically almost identical to LMoV, in L. longiflorum (Blom-Barnhoorn and van Aartrijk, 1985). Ozaki et al (1996) and Xu et al (2000) were successful in eradicating CMV in L. longiflorum through chemotherapy or in combination with callus culture.…”

Section: Introductionmentioning

confidence: 99%

Production of Virus-free Bulblets by Meristematic Tip Culture with Antiviral Chemical in Lilium brownii var. colchesteri

Masuda

Thien

Hải

et al. 2011

J. Japan. Soc. Hort. Sci.

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Lilium brownii var. colchesteri has unique and ornamental floral characteristics in graceful harmony with flower and anther colors, flower shape, elegant fragrance, and flower color change from yellowish cream to white during anthesis. There are, however, few accessions conserved in Japan up to the present, and they often show abnormally shaped flowers and mosaic leaves seemingly due to virus infection. Virus-free bulblets were established by combining meristematic tip culture and chemotherapy. At initial diagnosis with RT-PCR, Cucumber mosaic virus (CMV), Lily mottle virus (LMoV) and Lily symptomless virus (LSV) were detected from leaf tissues of the mother plants. All regenerated bulblets obtained by meristematic tip culture of the mother plants were still infected by at least one of the viruses. The meristematic tip of the bulblets infected with either LSV or LMoV was selected for subsequent culture with 2,4-dioxohexahydro-1,3,5-triazine (DHT), an antiviral chemical. LSV was eliminated successfully in mericlones from bulblets with LSV, whereas LMoV was not from those with LMoV. The virusfree bulblets were transferred to new medium without DHT, and multiplied by in vitro scaling. They were then acclimated in a phytotron glass room at 20°C. The plants were confirmed to be virus-free after 18-months' acclimation. It was concluded that the combination of meristem tip culture and chemotherapy is practical for producing virus-free plants of L. brownii var. colchesteri.

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The Regeneration of Plants Free of LSV and TBV From Infected Lilium Bulb-Scale Explants in the Presence of Virazole

Cited by 11 publications

References 0 publications

Production of high quality, healthy ornamental crops through meristem culture

Production of high quality, healthy ornamental crops through meristem culture

Elimination of ornithogalum mosaic virus in the Ornithogalum cv. Rogel through meristem tip culture and chemotherapy

Production of Virus-free Bulblets by Meristematic Tip Culture with Antiviral Chemical in Lilium brownii var. colchesteri

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