The regulation of liver gene expression by carbohydrates is mouse strain specific

Abstract

C57BL/6J and BALB/cJ mouse strains were analyzed by deep mRNA sequencing of the liver in the fasted state and following ingestion of standard laboratory mouse chow supplemented with plain drinking water or water containing 20% glucose, sucrose or fructose. Supplementation with these carbohydrates induced unique extents and temporal changes in gene expressions in a strain specific manner. Fructose and sucrose stimulated gene changes peaked at 3 h postprandial, whereas glucose effects peaked at 12 h postprandial in C57BL/6J mice and at 6 h postprandial in BABL/cJ mice. Network analyses revealed that fructose changed genes were primarily involved in lipid metabolism and were more complex in C57BL/6J than in BALB/cJ mice. These data demonstrate that there are qualitative and quantitative differences in the normal physiological responses of the liver between these two strains of mice and C57BL/6J is more sensitive to sugar intake than BALB/cJ.

Hepatic gene expression profiles during fed–fasted–refed state in mice

“…To date, several microarray profiling studies have been performed to investigate the Fed-Fast-Refed cycle. However, most transcriptomic studies in the liver have focused on a single aspect of the cycle, such as transition from fed to fasted or from fasted to refed states (Chi et al, 2020;Hwangbo et al, 2020;Wahl and LaRocca, 2021), which might be inadequate. Our study investigated the Fed-Fast-Refed cycle comprehensively and combined RNA-Seq with miRNA-Seq analysis.…”