The Regulation Role of PBK in Cell Cycle and Apoptosis of Prostate Cancer

Abstract

Objective: To investigate the effect of PBK on cell cycle and apoptosis of prostate cancer cell lines.Methods: Obtain the differences expression gene, PBK, which related to prostate cancer from high-throughput microarray, Furthermore, the cell cycle and apoptosis of prostate cancer cells were detected by flow cytometry after transfection with PBK inhibitor and overexpression plasmid.Results: After DU145/LNCaP transfection, the PBK expression levels were measured at 48 hours by RT-qPCR, compared to the blank control groups, the data showed the expression of PBK was significantly decreased in the groups of Si-PBK cell lines (DU145, the inhibition rate=84.78% P<0.01; LNCaP, the inhibition rate=94.79%, P<0.01); While the expression of PBK was significantly increased in the overexpression groups (DU145, fold=1.20, P<0.05; LNCaP, fold=1.35, P<0.05). The results of cell cycle analysis showed that after inhibition of the expression of PBK caused cell cycle arrest at the G0/G1 phase (LNCaP, P<0.05; DU145, P<0.01); On the contrary, a higher proportion of S, G2/M phase was shown in the over-expression-PBK DU145/LNCaP cell lines (LNCaP, P<0.05; DU145, P<0.01). Lastly, the cell apoptosis of DU145 and LNCaP cells was significantly increased after the inhibition of PBK expression (P<0.05) while an opposite result shown in the PBK overexpression cell lines (LNCaP, P<0.01; DU145, P<0.05). Conclusion: PBK can shorten the cell cycle and inhibit apoptosis of prostate cancer cells, which indicates that PBK can sever as a tumor promoter in prostate cancer. technology to carry out gene detection in 5 cases of PCa patients in

PBK is a potential therapeutic target and prognostic biomarker in ovarian cancer: integrating bioinformatics and clinical analysis

“…At the N-terminus of PBK protein there is an important phosphorylation site Thr-9 in which cdk1/cyclin B enables to activate. Moreover，the mitotic phosphorylation of Thr-9 is also involved in cytokinesis (Fang 2019). Domain analysis revealed that the Pkinase domain of PBK likely interacted with many other domains in the cell-cycle signaling pathway (Supplementary table).…”