The regulatory effect of flavonoids extracted from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways

Abstract

Context: ALI is a common disease characterized by acute pulmonary inflammatory disorder. Abutilon theophrasti Medik. (Malvaceae), as a Chinese traditional medicine, is used for the treatment of inflammation. Its main constituents are flavonoid compounds. Objective: This study investigates the regulatory effect of a TFE from Abutilon theophrasti leaves on gene expression in LPS-induced ALI mice via the NF-κB and MAPK signaling pathways. Materials and methods: Kunming mice were intragastrically administered TFE (0.25, 0.5, 1.0 g/kg) for 5 days, and then ALI was induced via intranasal administration 40 μg of LPS in 10 μL PBS after intragastric administration on the 5th day, and PBS and DEX (2 mg/kg) were negative and positive control groups, respectively. Results: The relative expression of iNOS gene was 0.707, 0.507 and 0.483 for 0.25, 0.5 and 1.0 g/kg TFE, and COX-2 gene expression was also reduced after treatment by three concentrations of TFE with 0.768, 0.545, and 0.478. The mRNA expression levels of p65 were 0.61, 0.43 and 0.27 for 0.25, 0.5 and 1.0 g/kg TFE and IκB levels were increased in a dose-dependent manner with 3.99, 13.69 and 34.36. 0.5 and 1.0 g/kg TFE inhibited the expression of ERK1/2 with 0.59 and 0.38, p38MAPK with 0.62 and 0.54, and JNK with 0.37 and 0.29, and JNK mRNA expression was 0.60 for 0.25 g/kg TFE. Discussion and conclusion: These results indicate that the regulatory mechanisms of TFE on gene expression in LPS-induced ALI mice include inhibition of the NF-κB and MAPK signaling pathways.

Ethnopharmacological and botanical evaluation of medicinal plants used by Brazilian Amazon Indian community

“…In addition to the diseases described above, numerous other conditions that affect the members of Cacoal community have been reported, such as snakebite accidents, for which Herrania mariae is employed. Similar to that observed with other plants reported in our study, no data corroborating this use were found, but according to data from Tian et al (2019), in a Alexandre Zandonadi Meneguelli; Ely Eduardo Saranz Camargo; Danieli Fernanda Buccini;…”

Anti-inflammatory Mechanism of Rhein in Treating Asthma Based on Network Pharmacology

“…In this study, we revealed a signalling nexus involving rhein, MAPK/NFκB pathway and in ammation in the HBE cells as shown in the summary diagram (Fig.7) .We have demonstrated that rhein can reduce the expression of phosphorylation-p38 and p65 induced by LPS+OVA via the MAPK/NF-κB pathway in HBE cells, and further reduce the in ammatory responses [37,38]. In the future, the anti-in ammatory effects of rhein need to be veri ed by further animal experiments.…”

Anti-inflammatory Mechanism of Rhein in Treating Asthma Based on Network Pharmacology

“…In this study, we revealed a signalling nexus involving rhein, MAPK/NFκB pathway and in ammation in the HBE cells as shown in the summary diagram( Figure.7) .We have demonstrated that rhein can reduce the expression of phosphorylation-p38 and p65 induced by LPS+OVA via the MAPK/NF-κB pathway in HBE cells, and further reduce the in ammatory responses [39,40]. In the future, the anti-in ammatory effects of rhein need to be veri ed by further animal experiments.…”