The Relation Between the P1A1/A2 Polymorphism of ITGB3 Gene and Myocardial Infarction in Elite Athletes

Abstract

A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve es s: : P1A1/A2 polymorphism on Integrin Subunit Beta 3 (ITGB3) gene's correlation with increased risk of myocardial infarction (MI) is not clear and various researches have reported contradictory results concerning the P1A1/A2 polymorphism of ITGB3 gene. We have focused on the relation P1A1/A2 polymorphism and MI, 26 male subjects under 45 years old who had MI. Due to the increasing concern of sudden death on athletes, we have compared these subjects with professional athletes. We aimed to investigate the frequency of P1A1/A2 polymorphism, in subjects under 45 years old suffering myocardial infarction (MI) and elite athletes who are at the same age range. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Peripheral blood was collected from 26 subjects with MI grouped as positive control, 25 healthy subjects grouped as negative control and 37 subjects as elite athletes. DNA was isolated from totally 88 peripheral blood samples. DNA extraction was followed by the PCR-RFLP method in order to detect the PlA1/A2 polymorphism. Amplified DNA products were digested with HpaII restriction enzyme. We aimed to see two bands, at 81 and 127 bp, after enzyme digestion. Standard descriptive statistical methods were applied to summarize the data. R Re e--s su ul lt ts s: : Three genotypes could be determined after assessment with bioanalyzer; genotype P1A1/P1A1 (81 and 127 bp fragments), genotype P1A1/P1A2 (81,127 and 208 bp fragments) and genotype P1A2/P1A2 (208 bp). Heterozygous polymorphic phenotype (P1A1/P1A2) was observed 8%, 7.7% and 17% on positive control group, negative control group, and elite athletes, respectively. Homozygous phenotype (PlA2/A2) was observed in 7.7% of positive control group only. C Co on nc cl lu us si io on n: : Our study highlights the significance of screening on athletes to prevent sudden deaths during sports. For next step, these obtained results will be analysed on larger populations to try to answer the question that "can sudden cardiac deaths (SCDs) be avoided and what should be done?" K Ke ey yw wo or rd ds s: : Myocardial infarction; polymorphism, genetic; ITGB3 protein, human Ö ÖZ ZE ET T A Am ma aç çl la ar r: : İntegrin Subünit Beta 3 (ITGB3) genindeki P1A1/A2 polimorfizmi ile miyokard infarktüsü (MI) artan riski ile ilişkisi net değildir ve çeşitli araştırmalar ITGB3 geninin P1A1/A2 polimorfizmi ile ilgili çelişkili sonuçlar bildirmiştir. P1A1/A2 polimorfizm ve MI arasındaki ilişkiye, 45 yaşın altındaki 26 erkek MI hastası üzerinde odaklandık. Atletlerde ani ölümle ilgili artan endişeler nedeniyle, bu örnekleri profesyonel sporcularla karşılaştırdık. Kırk beş yaş altı miyokard infarktüsü (MI) geçirmiş bireylerde ve aynı yaş aralığında elit atletlerde P1A1/ A2 polimorfizm sıklığını araştır-mayı amaçladık. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Pozitif kontrol grubu olarak MI hastası 26 örnekten, negatif kontrol olarak gruplandırılan 25 sağlıklı örnekten ve elit atlet grubu olarak 37 örnekten periferik kan top...

Clinical Significance of Platelet Antigen 1/Platelet Antigen 2 (PlA1/A2) Polymorphism of Integrin b3 (ITGB3) Gene in Coronary Artery Disease

“…As the CAD is a complex multifactorial disease, the PlA role in the pathogenesis of CAD is largely dependent on other factors that influence the impact of any altered PlA phenotype and the disease outcome depends on the sum of other multiple interacting factors determining the disease severity [2] . Thus, the identification of a contribution by a single gene polymorphism to a multifactorial, polygenic pathological process is challenging and requires a very large sample size [19] .…”