The relation of phage pattern and lysogenicity in the phage typing of staphylococci of phage-group II

Winkler, Kurt; Grootsen, C.

doi:10.1007/bf02538445

Cited by 9 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…) radiation (Morse, 1962). The phages in lytic group I1 described by Winkler & Grootsen (1961) were arranged in three groups, y, 6 and e according to their lytic spectra on fifty strains. The phages within each group are probably coimmune.…”

Section: Methodsmentioning

confidence: 99%

Lysogenic Conversion of Staphylococci to Loss of -Toxin

Winkler¹,

Waart²,

Grootsen³

et al. 1965

Journal of General Microbiology

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Lysogenic Conversion of Staphylococci to Loss of -Toxin

Winkler¹,

Waart²,

Grootsen³

et al. 1965

Journal of General Microbiology

Self Cite

View full text Add to dashboard Cite

“…The majority of clinical isolates possess one or more temperate phages, presumably as prophages integrated into the chromosome, the presence of which may also affect the phage-typing pattern (27,417,544); lysogenicity patterns combined with phage types have been used for epidemiological and evolutionary comparisons of clinical isolates (27, 526a). The lysogenic status of a strain has also been reported to affect its ability to engage in gene transfer via transformation (422), transduction (81, 82, 84; however, see reference 476), and mixed culture (251, 256).…”

Section: Staphylococcal Bacteriophages and Transductionmentioning

confidence: 99%

Antimicrobial resistance of Staphylococcus aureus: genetic basis.

Lyon¹,

Skurray²

1987

Microbiological Reviews

382

217

View full text Add to dashboard Cite

“…Smith and Condemine (36) stated that "technology needs to be developed to simplify map construction by using maps that already exist for related organisms." This project outlines one such technology that was applied to Staphylococcus aureus.The species is divided into five phage groups on the basis of typing patterns produced on infection with staphylococcal phages (23,32,33,42,45,48). A detailed genetic and physical map has been established in the phage group III S. aureus NCTC 8325 by using methods of classical genetics and pulsedfield gel electrophoresis (PFGE) analysis (27,28,31).…”

mentioning

confidence: 99%

Construction of a chromosome map for the phage group II Staphylococcus aureus Ps55

Bannantine

Pattee

1996

J Bacteriol

View full text Add to dashboard Cite

The genome size and a partial physical and genetic map have been defined for the phage group II Staphylococcus aureus Ps55. The genome size was estimated to be 2,771 kb by pulsed-field gel electrophoresis (PFGE) using the restriction enzymes SmaI, CspI, and SgrAI. The Ps55 chromosome map was constructed by transduction of auxotrophic and cryptic transposon insertions, with known genetic and physical locations in S. aureus NCTC 8325, into the Ps55 background. PFGE and DNA hybridization analysis were used to detect the location of the transposon in Ps55. Ps55 restriction fragments were then ordered on the basis of genetic conservation between the two strains. Cloned DNA probes containing the lactose operon (lac) and genes encoding staphylococcal protein A (spa), gamma hemolysin (hlg), and coagulase (coa) were also located on the map by PFGE and hybridization analysis. This methodology enabled a direct comparison of chromosomal organization between NCTC 8325 and Ps55 strains. The chromosome size, gene order, and some of the restriction sites are conserved between the two phage group strains.A physical map of a chromosome consists of an ordered set of restriction fragments that places genomic sequence in the order it exists inside the cell. The resolution of chromosome structure can vary from a macrorestriction map consisting of only a few large, restriction fragments (10, 14, 17, 37) to thousands of small clones of ordered chromosomal DNA (4, 13) to a complete nucleotide sequence (11). The advantages of a physical map over a genetic map are severalfold. Physical maps are faster to construct and require considerably less laborintensive methods. Methods in genetic mapping, such as conjugation, transformation, transduction, and mutagenesis, can be bypassed in physical map construction. Many prokaryotic physical maps have been constructed as a starting point to characterize the chromosome because methods of genetic analysis were underdeveloped (1,2,5,14,15,17,43,47). Other physical maps were constructed to confirm previously established genetic maps (10,28,46).With the establishment of long-range genomic mapping techniques (20,36), there has been a recent surge in the field of prokaryotic genomic mapping. With these maps constructed, a preliminary assessment of chromosomal diversity among the prokaryotes may become possible. This assessment of chromosomal diversity has already been examined for species in the genus Neisseria (8), where a newly constructed Neisseria meningitidis map was compared with a previously constructed map of a gonococcal strain. Smith and Condemine (36) stated that "technology needs to be developed to simplify map construction by using maps that already exist for related organisms." This project outlines one such technology that was applied to Staphylococcus aureus.The species is divided into five phage groups on the basis of typing patterns produced on infection with staphylococcal phages (23,32,33,42,45,48). A detailed genetic and physical map has been established in the phage group III S. aure...

show abstract

The relation of phage pattern and lysogenicity in the phage typing of staphylococci of phage-group II

Cited by 9 publications

References 7 publications

Lysogenic Conversion of Staphylococci to Loss of -Toxin

Lysogenic Conversion of Staphylococci to Loss of -Toxin

Antimicrobial resistance of Staphylococcus aureus: genetic basis.

Construction of a chromosome map for the phage group II Staphylococcus aureus Ps55

Contact Info

Product

Resources

About