The amino‐acids of alcoholic extracts of lucerne hay cut in different stages of growth and mature grass and legume hays are estimated quantitatively by a rapid approximate paper chromatographic method. It is found that the amino‐acids of the lucerne cut in varying stages of growth decline in quantity with increasing age, although some of the amino‐acids form a fairly constant percentage of the total nitrogen at each stage. Differences in asparagine‐aspartic acid content of lucerne hay from two different sources, as well as those of glycine, serine, alanine and proline, caused by manuring, are noted. Certain family amino‐acid differences are observed among the mature hays. The alcoholic extracts of fenugreek seeds are found to contain few free amino‐acids; other acids exist in peptide combination.