The Relationship Between EBV Virus and Breast Cancer in Khuzestan Province of Iran

Saeedi, Zahra; Hadi, Faranak; Hejazi, Seyed Hesamaldin; Salahshournia, Zahra

doi:10.29252/jabr.01.01.07

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…53 In contrast, a low percentage of EBV DNA was also found in 5.12% of FFPE tissues of Iranian women. 54 However, EBV DNA was not detected in FFPE tissues of Iranian women using PCR targeting the Bam HI-H rightward open reading frame 1 (BHRF1) region. 55 Moreover, EBV DNA was detected using PCR in 20% of fresh tissues of Egyptian BC patients, 56 4.7% of Mexican BC patients, 57 and 31% of Argentinian BC patients.…”

Section: Discussionmentioning

confidence: 99%

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

Metwally¹,

Abo-Shadi²,

Fattah³

et al. 2021

IDR

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Background Oncogenic viruses, their possible association with breast cancer (BC) and effect on its clinical course are interesting issue. The present study evaluates the presence of human papillomavirus (HPV), EpsteinBarr virus (EBV), and human mammary tumor virus (HMTV) in BC and their relation with clinico-pathological characteristics. Patients and Methods This study was conducted on 80 Egyptian women with BC and 30 control women without known oncological disease. Forty formalin-fixed paraffin-embedded (FFPE) tissues, forty fresh tissue samples, and white blood cells (WBCs) of BC patients and WBCs of controls were subjected to a qualitative polymerase chain reaction (PCR). Quantitative real-time PCR was used to measure viral loads in fresh tissues of BC. The result was correlated with clinico-pathological characteristics of BC. Results HPV was detected in 33 (41.25%), EBV in 30 (37.5%) and HMTV in 33 (41.25%) BC patients. None of the control women was positive for HPV or EBV while HMTV was detected in 7 (23.3%). Among 40 BC WBCs specimens, HPV/HMTV were found together in 25%, followed by EBV/HMTV in 2.5% and EBV/HPV in 2.5%. However, the three viruses (HPV/EBV/HMTV) were found together in only 5%. In the 40 fresh BC tissues, the three viruses were found together in 12 (30%), EBV/HMTV in 7 (17.5%), HPV/HMTV in 4 (10%), and HPV/EBV in 4 (10%). EBV, HMTV, or multiple viral infections were associated with younger age of BC women. HPV, EBV, and HMTV median loads in fresh tissues were 4.8×10 3 copies/μL, 6.3×10 3 copies/μL, and 97 copies/μL, respectively. Conclusion WBCs could be a more suitable specimen instead of fresh tissue for HMTV detection in BC patients to avoid invasive procedures. The presence of HPV, EBV, and HMTV together in Egyptian women with BC was significantly associated with younger age.

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Section: Discussionmentioning

confidence: 99%

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

Metwally¹,

Abo-Shadi²,

Fattah³

et al. 2021

IDR

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“…Overall, 6.8% (7/102) of samples comprised 11.76% (6/51) in malignant tissues and 1.9% (1/51) in adjacent non-malignant tissues found to be positive for presence of EBV genome, which was statistically significant (P<0.05).Similar to our results, several case-control reports from Iran and other parts of the world have demonstrated higher prevalence of EBV in malignant tumors as compared with control groups. In several studies, the prevalence rate of EBV in malignant breast tissues and nonmalignant control groups were as follows: 9.3% and 0% in a study by Aghdam et al (Iran, 2017), 8% and 0% in a study by Malekpour et al (Iran, 2015), 5.1% and 0% in a study by Saeedi et al (Iran, 2018), 10% and 0% in a study by Antonsson et al (Australia, 2012), 13.6% and 4.5% in a study by Ladera et al (Venezuela, 2017), respectively (28)(29)(30)(31)(32). It is noteworthy that despite other investigations, our study used adjacent non-malignant tissues to improve the accuracy of viral causality in the development of malignancy.…”

Section: Discussionmentioning

confidence: 91%

MiRNA-218 Is Frequently Downregulated in Malignant Breast Tumors: A Footprint of Epstein-Barr Virus Infection

et al. 2021

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Scan to discover onlineBackground & Objective: The role of Epstein-Barr Virus in development of breast cancer is frequently studied. In this regard, miRNAs are among the contributing elements in the molecular pathophysiology of EBV-related diseases. In addition, a growing number of host miRNAs are believed to be implicated in pathogenesis of breast cancer. MiR-218 is a tumor suppressive miRNA that is subjected to dysregulation in various EBV-associated cancers. We aimed to investigate the frequency of EBV and its relationship with expression status of tumor suppressive miR-218 in breast cancer and adjacent normal tissue.Methods: A total number of 51 fresh malignant breast cancer tissues (cases) and their adjacent normal tissues (controls) were collected. Nested-PCR and RT-qPCR were set to identify EBV frequency and miR-218 expression in cases and controls, respectively.Results: Out of all samples, 6.8% (7/102) comprising 11.6% (6/51) in malignant tissues and 1.9% (1/51) in normal control tissues were positive for EBV (P<0.05). Quantitative data showed that miR-218 was significantly downregulated in malignant tissues compared to control tissues (P<0.0001). In addition, reduced expression of miR-218 was associated with adverse clinical outcomes, metastasis, and higher grades of malignancy. Given the presence of EBV, lower expression of miR-218 was observed in breast cancer group in comparison with normal group (P<0.05). Conclusion:Our results raise the possibility of the relation between EBV infection and miR-218 downregulation in breast cancer and propose further investigations in this regard.

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“…In this study, they reported that EBV was found in 5.12% of breast cancer tissue but reported that there were no significant relationships between breast cancer and EBV. [ 30 ] These differences could be justified based on the regional differences. In 2021, a study was performed by Sinclair et al .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Relative Frequency of Epstein–Barr Virus Infection in Patients with Recurrent Breast Cancer Compared with Patients with Nonrecurrent Breast Cancer

Samani

Safaee

Nematollahi

et al. 2023

Advanced Biomedical Research

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Background: The roles of Epstein–Barr virus (EBV) in breast cancer and breast lymphoma by transfecting EBV DNA have been indicated in different studies, but few investigations have been conducted on its roles in recurrence of breast cancer. Here, we aimed to evaluate the roles of EBV in recurrent breast cancer tissue. Materials and Methods: This is a cross-sectional retrospective study that was performed in 2020–2021 in Isfahan on patients with breast cancer. The study population consisted of 30 tissue samples from recurrent breast cancer and 30 samples from nonrecurrent breast cancer. We collected demographic data of patients including age using a checklist. Other collected data were type of cancer, stages of cancer, tumor size in greatest dimension, lymph node involvements, and presence of metastasis. Furthermore, we evaluated all of the pathology samples from both groups for the presence of DNA of EBV and compared the data of both groups. Results: The DNA of EBV was positive in 8 patients of the relapsed group (26.6%) and 7 patients in the nonrelapsed patients (23.3%). There was no significant difference between two groups regarding positive DNA of EBV (P = 0.39). There were no significant differences between two groups of positive DNA of EBV with and without recurrent breast cancer regarding type of cancer (P = 0.63), stage of cancer (P = 0.19), tumor size in greatest dimension (P = 0.31), mean lymph node involvement (P = 0.27), number of lymph node involvement (P = 0.43), and metastasis (P = 0.69). Conclusion: EBV might have no significant role in recurrence of breast cancer.

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The Relationship Between EBV Virus and Breast Cancer in Khuzestan Province of Iran

Cited by 4 publications

References 41 publications

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

Presence of HPV, EBV and HMTV Viruses Among Egyptian Breast Cancer Women: Molecular Detection and Clinical Relevance

MiRNA-218 Is Frequently Downregulated in Malignant Breast Tumors: A Footprint of Epstein-Barr Virus Infection

Evaluation of the Relative Frequency of Epstein–Barr Virus Infection in Patients with Recurrent Breast Cancer Compared with Patients with Nonrecurrent Breast Cancer

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