The relationship between embb306 and embb406 mutations and ethambutol resistant in Mycobacterium tuberculosis isolated from patiens in west of Iran

Mohammadi, Bahman; Mohajeri, Parviz; Rouhi, Samaneh; Ramazanzadeh, Rashid

doi:10.14196/mjiri.32.117

Cited by 4 publications

(6 citation statements)

References 11 publications

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“…The MDR-TB rate is 11% in India [ 22 ]. In a previous study, the MDR rate was higher than in our study [ 23 , 24 ]. Therefore, TB control was successful in the west of Iran.…”

Section: Discussioncontrasting

confidence: 83%

Molecular epidemiology of Mycobacterium tuberculosis isolates in Iran using spoligotyping

Ramazanzadeh

Shakib

Rouhi

et al. 2020

New Microbes and New Infections

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Spoligotyping can help assess the transmission of Mycobacterium tuberculosis strains. We aimed to study the genotyping of M. tuberculosis isolated from patients with tuberculosis from the west of Iran by spoligotyping. Forty-seven M. tuberculosis isolates were collected from the west of Iran. All samples were cultured on Löwenstein-Jensen medium incubated at 37°C for 8 weeks. Bacterial isolates were identified as M. tuberculosis using standard biochemical tests. Drug resistance patterns of M. tuberculosis to rifampicin and isoniazid were determined, and multidrug-resistant (MDR) strains were isolated. After DNA extraction, spoligotyping was performed. We found new spoligotypes 4162 and 4163, which correlated with atypical lineage. Atypical and unknown lineages also had correlations with the MDR tuberculosis rate (4%). The most prevalent spoligointernational types were orphan (34%), 2669 (23.4%) and 127 (14.8%) types. The most prevalent clades were Ural-2 (NEW-1) (25.53%) and atypical (23.40%) lineages. The predominant clade was Ural-2 (NEW-1) and an atypical lineage restricted to Iran. The rate of MDR was low. Knowledge of the circulating isolates in the west of Iran will help implement control programmes, so knowledge of the dynamic transmission of local isolates is crucial.

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“…The MDR-TB rate is 11% in India [ 22 ]. In a previous study, the MDR rate was higher than in our study [ 23 , 24 ]. Therefore, TB control was successful in the west of Iran.…”

Section: Discussioncontrasting

confidence: 83%

Molecular epidemiology of Mycobacterium tuberculosis isolates in Iran using spoligotyping

Ramazanzadeh

Shakib

Rouhi

et al. 2020

New Microbes and New Infections

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“…Mutations in the embB gene can cause EMB resistance by a mechanism of drug target alteration [6]. In previous studies, the embB mutation rates in EMB r isolates varied widely, from 10 [50] to 100% [23]. In this study, 62.5% of the EMB r isolates mutated in embB, closer to Bakula et al's finding, which focused only on MDR isolates [17].…”

Section: Discussionsupporting

confidence: 72%

“…Single or combined resistance to INH, RIF, and EMB was detected in 54.2% (n 5 13), 66.7% (n 5 16), and 33.3% (n 5 8) of isolates, respectively. MDR isolates accounted for 6 2 8C R: 5 0 -TACGGCGTTTCGATGAACC-3 0 embB F: 5 0 -CGACGCCGTGGTGATATTCG-3 0 863 bp [23] 61.5 8C R: 5 0 -CCACGCTGGGAATTCGCTTG-3 0 ubiA F: 5 0 -ACGTTGAGCTTGAGGCTAGC-3 0 909 bp [24] 61.6 8C R: 5 0 -CGCTGTCGCGAATACTGCT-3 0 pncA F: 5 0 -CGGATTTGTCGCTCACTACA-3 0 756 bp [25] 6 2 8C R: 5 0 -TCCGCCGCCGAACAGTTCATCCCGGT-3 0 IS6110 F: 5 0 -CTCGTCCAGCGCCGCTTCGG-3 0 130 bp [26] 6 3 8C R: 5 0 -CCTGCGAGCGTAGGCGTCGG-3 0 ) of all RIF r cases. Statistically significant, all isolates belonging to the NEW-1 sub-lineage were mono-resistant (P-value 5 0.0137).…”

Section: Resultsmentioning

confidence: 99%

Identification of mutations in rpoB, pncA, embB, and ubiA genes among drug-resistant Mycobacterium tuberculosis clinical isolates from Iran

Bakhtiyariniya

Khosravi

Hashemzadeh

et al. 2022

AMicr

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Mycobacterium tuberculosis resistant to effective first-line drugs (FLDs) has challenged national and global tuberculosis control programs. This study aimed to identify mutations in 4 genes related to rifampin, pyrazinamide, and ethambutol resistance among clinical isolates of M. tuberculosis from southwestern Iran. After drug susceptibility testing of 6620 M. tuberculosis clinical isolates by proportional method, a total of 24 FLD-resistant strains were included in the study. Fragments of rpoB, pncA, embB, and ubiA genes were amplified and sequenced to mine the mutations by pairwise alignment with the corresponding M. tuberculosis H37Rv genes. Phenotypic resistance to rifampin, isoniazid, and ethambutol was detected in 67, 54, and 33% (n = 16, 13, and 8) of the isolates, respectively. Of rifampin-resistant isolates, 31% (5/16) were mono-resistant, and 56% (9/16) were multidrug-resistant (MDR). In 100% of rifampin-resistant isolates, mutations were found in the rifampin resistance-determining region (RRDR) of the rpoB, with S450L substitution being the most common, especially in MDRs (77.8%, 7/9). Resistance-conferring mutations in pncA were present in 12.5% (3/24) of FLD-resistant isolates. The embB and ubiA mutations were found in 62.5 and 12.5% (5/8 and 1/8) of ethambutol-resistant isolates, respectively, of which the embB D354A was the most common substitution (37.5%, 3/8). Sixteen distinct mutations were identified, one of which was novel. The sequence analysis of the RRDR segment was the best way to detect rifampin resistance. The rpoB S450L substitution could be a helpful molecular marker to predict MDR. In other genes, no mutation was identified as a reliable marker.

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“…This drug was first found effective in 1961. [ 7 8 ] EMB's target is the cell wall and this drug interferes with arabinosyl transferase. This enzyme is coded by embCAB operon and it is associated with the biosynthesis of arabinogalactan and lipoarabinomannan.…”

Section: Introductionmentioning

confidence: 99%

“…[ 13 ] Using the Lowenstein-Jensen proportion method and polymerase chain reaction (PCR)-sequencing, it was observed that 14% of M. tuberculosis strains were resistant to EMB in Iran and 85.71% of the resistant isolates had embB 306 mutations. [ 7 ] In another study in Iran using Lowenstein-Jensen proportion method, PCR- single-strand conformation analysis (SSCP; is a post-PCR technique that can be used to screen for mutations that are not limited to a single hot spot but are randomly distributed throughout the exons) and direct sequencing, it was observed that 2/32 of M. tuberculosis strains were resistant to EMB. The sequencing of the embB 306 gene in this study showed that both isolates of EMB-resistant M. tuberculosis were mutated.…”

Section: Introductionmentioning

confidence: 99%

Frequency of Codon 306 Mutations in embB Gene of Mycobacterium tuberculosis Resistant to Ethambutol: A Systematic Review and Meta-Analysis

et al. 2020

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Background: Ethambutol (EMB) resistance is a major concern in patients with tuberculosis (TB). The aim of this study was to determine the frequency rate of mutations in the embB 306 gene of Mycobacterium tuberculosis ( M. tuberculosis ) resistant to EMB, based on a systematic review and meta-analysis. Methods: Thirty-seven original articles (1997–2015) that have been published in valid databases were considered for this research. The articles were systematically reviewed for the prevalence and rate of mutations in embB 306 in EMB-resistant M. tuberculosis . Data were analyzed using meta-analysis and random effects models (CI 95%, P < 0.10). Results: With a 6,931 sample size in 37 original articles, the lowest rate was related to EMB resistance that was observed in 2014 with 0.05 (95% CI: 0.04–0.07) and the highest prevalence rate was 0.84 (95% CI: 0.68–1.01), observed in 1997. Lowest and highest prevalence rates of embB 306 gene mutation in M. tuberculosis were 0.03 (95% CI: 0.01–0.07) in 2014 and 0.78 (95% CI: 0.71–1.84) in 2005, in the USA, respectively. Conclusions: The present study revealed the prevalence and association of mutations in the embB 306 gene of M. tuberculosis with resistance to EMB. Detecting EMB-resistant M. tuberculosis can help in controlling and correcting the administration of drugs for patients with TB.

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The relationship between embb306 and embb406 mutations and ethambutol resistant in Mycobacterium tuberculosis isolated from patiens in west of Iran

Cited by 4 publications

References 11 publications

Molecular epidemiology of Mycobacterium tuberculosis isolates in Iran using spoligotyping

Molecular epidemiology of Mycobacterium tuberculosis isolates in Iran using spoligotyping

Identification of mutations in rpoB, pncA, embB, and ubiA genes among drug-resistant Mycobacterium tuberculosis clinical isolates from Iran

Frequency of Codon 306 Mutations in embB Gene of Mycobacterium tuberculosis Resistant to Ethambutol: A Systematic Review and Meta-Analysis

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