The relationship between genetic and cytogenetic maps of pea. II. Physical maps of linkage mapping populations

Hall, Kat; Parker, John; Ellis, Noel; Turner, L.; Knox, M. R.; Hofer, Julie; Lü, Jinying; Ferrándiz, Cristina; Hunter, P. J.; Taylor, Julian; Baird, Kathleen

doi:10.1139/g97-798

Cited by 58 publications

(66 citation statements)

References 17 publications

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“…recombinant-inbred-line population derived from the cross JI 281 ϫ JI 399 (Hall et al, 1997). PEASQUA was found to be 99% identical to PEAM4, an independently isolated pea homolog of AP1 and SQUA (Berbel et al, 2001), although it is 10 bp longer than PEAM4 in the 5Ј-untranslated region and 63 bp longer in the 3Ј-untranslated region.…”

Section: Pim Mutations Delay Floral Meristem Specification and Alter mentioning

confidence: 94%

PROLIFERATING INFLORESCENCE MERISTEM, a MADS-Box Gene That Regulates Floral Meristem Identity in Pea

et al. 2002

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SQUAMOSA and APETALA1 are floral meristem identity genes from snapdragon (Antirrhinum majus) and Arabidopsis, respectively. Here, we characterize the floral meristem identity mutation proliferating inflorescence meristem(pim) from pea (Pisum sativum) and show that it corresponds to a defect in the PEAM4 gene, a homolog of SQUAMOSA and APETALA1. ThePEAM4 coding region was deleted in thepim-1 allele, and this deletion cosegregated with thepim-1 mutant phenotype. The pim-2 allele carried a nucleotide substitution at a predicted 5′ splice site that resulted in mis-splicing of pim-2 mRNA. PCR products corresponding to unspliced and exon-skipped mRNA species were observed. The pim-1 and pim-2 mutations delayed floral meristem specification and altered floral morphology significantly but had no observable effect on vegetative development. These floral-specific mutant phenotypes and the restriction ofPIM gene expression to flowers contrast with other known floral meristem genes in pea that additionally affect vegetative development. The identification of PIM provides an opportunity to compare pathways to flowering in species with different inflorescence architectures.

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Section: Pim Mutations Delay Floral Meristem Specification and Alter mentioning

confidence: 94%

PROLIFERATING INFLORESCENCE MERISTEM, a MADS-Box Gene That Regulates Floral Meristem Identity in Pea

et al. 2002

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“…The dne-1 mutation was detected as a cleaved amplified polymorphic sequence (marker, and cosegregation with the dne phenotype was confirmed in segregating progenies from several different crosses. For mapping of Ps ELF4/DNE, a polymorphism was identified and scored as a derived cleaved amplified polymorphic sequence marker in the JI281 3 JI399 recombinant inbred line population (Hall et al, 1997). All primer details are given in Supplemental Table 1 online.…”

Section: Gene Isolation Mapping and Molecular Genotypingmentioning

confidence: 99%

DIE NEUTRALIS and LATE BLOOMER 1 Contribute to Regulation of the Pea Circadian Clock

et al. 2009

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The DIE NEUTRALIS (DNE) locus in garden pea (Pisum sativum) was previously shown to inhibit flowering under noninductive short-day conditions and to affect a graft-transmissible flowering signal. In this study, we establish that DNE has a role in diurnal and/or circadian regulation of several clock genes, including the pea GIGANTEA (GI) ortholog LATE BLOOMER 1 (LATE1) and orthologs of the Arabidopsis thaliana genes LATE ELONGATED HYPOCOTYL and TIMING OF CHLOROPHYLL A/B BINDING PROTEIN EXPRESSION 1. We also confirm that LATE1 participates in the clock and provide evidence that DNE is the ortholog of Arabidopsis EARLY FLOWERING4 (ELF4). Circadian rhythms of clock gene expression in wild-type plants under constant light were weaker in pea than in Arabidopsis, and a number of differences were also seen in the effects of both DNE/ELF4 and LATE1/GI on clock gene expression. Grafting studies suggest that DNE controls flowering at least in part through a LATE1-dependent mobile stimulus, and dne mutants show elevated expression of a FLOWERING LOCUS T homolog under short-day conditions. However, the early flowering of the dne mutant is not associated with altered expression of a previously described CONSTANS-like gene. Collectively, our results characterize the clock system and reveal its importance for photoperiod responsiveness in a model legume.

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“…PCR fragments were cloned in pGEM-T (Promega) and sequenced at the Australian Genome Research Facility (Brisbane, Australia). A single nucleotide polymorphism in intron 4 of LONG1 between lines JI281 and JI399 was converted to a cleaved amplified polymorphic sequence (CAPS) marker (HaeII site in JI281) and used to map the gene in a JI281 3 JI399 recombinant inbred line population (Hall et al, 1997). The long1-1 point mutation was converted into a derived CAPS marker, introducing a diagnostic NcoI site.…”

Section: Sequence Isolation Mapping and Molecular Markersmentioning

confidence: 99%

Light Regulation of Gibberellin Biosynthesis in Pea Is Mediated through the COP1/HY5 Pathway

et al. 2009

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Light regulation of gibberellin (GA) biosynthesis occurs in several species, but the signaling pathway through which this occurs has not been clearly established. We have isolated a new pea (Pisum sativum) mutant, long1, with a light-dependent elongated phenotype that is particularly pronounced in the epicotyl and first internode. The long1 mutation impairs signaling from phytochrome and cryptochrome photoreceptors and interacts genetically with a mutation in LIP1, the pea ortholog of Arabidopsis thaliana COP1. Mutant long1 seedlings show a dramatic impairment in the light regulation of active GA levels and the expression of several GA biosynthetic genes, most notably the GA catabolism gene GA2ox2. The long1 mutant carries a nonsense mutation in a gene orthologous to the ASTRAY gene from Lotus japonicus, a divergent ortholog of the Arabidopsis bZIP transcription factor gene HY5. Our results show that LONG1 has a central role in mediating the effects of light on GA biosynthesis in pea and demonstrate the importance of this regulation for appropriate photomorphogenic development. By contrast, LONG1 has no effect on GA responsiveness, implying that interactions between LONG1 and GA signaling are not a significant component of the molecular framework for light–GA interactions in pea.

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The relationship between genetic and cytogenetic maps of pea. II. Physical maps of linkage mapping populations

Cited by 58 publications

References 17 publications

PROLIFERATING INFLORESCENCE MERISTEM, a MADS-Box Gene That Regulates Floral Meristem Identity in Pea

PROLIFERATING INFLORESCENCE MERISTEM, a MADS-Box Gene That Regulates Floral Meristem Identity in Pea

DIE NEUTRALIS and LATE BLOOMER 1 Contribute to Regulation of the Pea Circadian Clock

Light Regulation of Gibberellin Biosynthesis in Pea Is Mediated through the COP1/HY5 Pathway

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