The relationship between lethal mutation yield and intake of ethylnitrosourea (ENU) in <i>Drosophila melanogaster</i>

Ayaki, Toshikazu; Ohshima, Kayoko; Okumura, Yutaka; Yoshikawa, Isao; Shiomi, Toshio

doi:10.1002/em.2860060402

Cited by 8 publications

(3 citation statements)

References 5 publications

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“…consume the same amount of food at each level of mutagen, and second the amount of food consumed may vary from experiment to experiment. Various attempts to quantitate mutagen consumption have been reported [Gollapudi et al, 1985;Ayaki et al, 1984;Yoshikawa et al, 1984;Thomp-The quantitation of mutagenic responses to multiple exposures of a mutagen in feeding studies with Drosophilu is complicated by two main factors. First, the flies may not son and Reeder, 19871.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of a method for quantitating food consumption for mutation assays in Drosophila

Thompson

Reeder

Bruce

1991

Environ and Mol Mutagen

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Quantitation of food consumption is necessary when determining mutation responses to multiple chemical exposures in the sex-linked recessive lethal assay in Drosophila. One method proposed for quantitating food consumption by Drosophila is to measure the incorporation of 14C-leucine into the flies during the feeding period (Thompson and Reeder: Environmental Mutagenesis 10:357-365, 1987). Three sources of variation in the technique of Thompson and Reeder have been identified and characterized. First, the amount of food consumed by individual flies differed by almost 30% in a 24 hr feeding period. Second, the variability from vial to vial (each containing multiple flies) was around 15%. Finally, the amount of food consumed in identical feeding experiments performed over the course of 1 year varied nearly 2-fold. The use of chemical consumption values in place of exposure levels provided a better means of expressing the combined mutagenic response. In addition, the kinetics of food consumption over a 3 day feeding period for exposures to cyclophosphamide which produce lethality were compared to non-lethal exposures. Extensive characterization of lethality induced by exposures to cyclophosphamide demonstrate that the lethality is most likely due to starvation, not chemical toxicity.

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Section: Discussionmentioning

confidence: 99%

“…Yoshikawa I, Ayaki T, Ohshima K (1984): Comparative studies of dose-response curves for recessive lethal mutations induced by ethylnitrosouria in spermatogonia of Drosophila melunoguster. Environ Mutagen 6:489496.…”

Section: Quantitation Of Food Consumption For Drosophila 21mentioning

confidence: 99%

Characterization of a method for quantitating food consumption for mutation assays in Drosophila

Thompson

Reeder

Bruce

1991

Environ and Mol Mutagen

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“…As an alternative to dyes, the medium can be supplemented with radioactively labeled substances such as [ 14 C]-choline, [ 3 H]-thymidine or [α-32P]-dCTP [ 57 , 58 , 59 , 60 , 61 , 62 , 63 ]. Similar to the methods using dyed medium, flies are fed on labeled medium for a certain period of time.…”

Section: Phenotyping Of D Melanogastermentioning

confidence: 99%

Phenotyping of Drosophila Melanogaster—A Nutritional Perspective

et al. 2022

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The model organism Drosophila melanogaster was increasingly applied in nutrition research in recent years. A range of methods are available for the phenotyping of D. melanogaster, which are outlined in the first part of this review. The methods include determinations of body weight, body composition, food intake, lifespan, locomotor activity, reproductive capacity and stress tolerance. In the second part, the practical application of the phenotyping of flies is demonstrated via a discussion of obese phenotypes in response to high-sugar diet (HSD) and high-fat diet (HFD) feeding. HSD feeding and HFD feeding are dietary interventions that lead to an increase in fat storage and affect carbohydrate-insulin homeostasis, lifespan, locomotor activity, reproductive capacity and stress tolerance. Furthermore, studies regarding the impacts of HSD and HFD on the transcriptome and metabolome of D. melanogaster are important for relating phenotypic changes to underlying molecular mechanisms. Overall, D. melanogaster was demonstrated to be a valuable model organism with which to examine the pathogeneses and underlying molecular mechanisms of common chronic metabolic diseases in a nutritional context.

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Method for selecting exposure levels for the Drosophila sex‐linked recessive lethal assay

Thompson

Reeder

1987

Environ and Mol Mutagen

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The use of the Drosophila sex-linked recessive lethal assay for detecting mutagenicity of chemicals is well established. When compounds are tested by feeding adult flies, the National Toxicology Program protocol specifies a 3-day feeding regimen at an exposure level that produces about 30% mortality. Uptake of the test compound is monitored by feeding behavior, amount of excretion, or abdomen size (Woodruff et al: Environ Mutat 7:677-702, 1985). An alternate method for determining uptake is to add radiolabeled sucrose to the feeding solution and then to determine the amount of radioactivity in the flies (Gollapudi et al: Mutat Res 144:13-17, 1985). We have found that the addition of radiolabeled sucrose underestimates consumption for feeding exposures longer than 24 hr because sucrose is metabolized and as much as 30% of the label is excreted, presumably as 14CO2 or 3H2O. Here we describe a method for determining uptake of chemicals by adding 14C-leucine to the feeding solution. The incorporation of 14C-leucine is essentially linear over the 3-day feeding period, which permits accurate estimates of food consumption. Use of this method demonstrates that lower exposure levels of a chemical that do not produce mortality actually results in higher consumption by the flies. The method is proposed as a prescreen to select the appropriate exposure level for the sex-linked recessive lethal assay.

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The relationship between lethal mutation yield and intake of ethylnitrosourea (ENU) in Drosophila melanogaster

Cited by 8 publications

References 5 publications

Characterization of a method for quantitating food consumption for mutation assays in Drosophila

Characterization of a method for quantitating food consumption for mutation assays in Drosophila

Phenotyping of Drosophila Melanogaster—A Nutritional Perspective

Method for selecting exposure levels for the Drosophila sex‐linked recessive lethal assay

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