The relationship between oxidative stress and preeclampsia. The serum ischemia-modified albumin levels and thiol/disulfide homeostasis

Onat, Taylan; Kırmızı, Demet Aydoğan; Başer, Emre; Ercan, Müjgan; Yalçın, Serenat Eriş; Kara, Mustafa; Esinler, Deniz; Yalvaç, Ethem Serdar

doi:10.4274/tjod.galenos.2020.23682

Cited by 18 publications

(11 citation statements)

References 38 publications

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“…Consequently, the investigation of serum IMA generated by hypoxia/ischemia-driven oxidative stress can be useful in this matter. 3,25 In our study, we observed significantly elevated serum levels of IMA in severe PE as compared with mild PE and control groups (P 0.001). Our results agreed with those of the study done by Karasin and Cift, 2020, 25 which showed that inflammation and endothelial cell activity in PE may alter the albumin molecule in the plasma of preeclamptic patients and may cause an increase in its levels.…”

Section: Discussionsupporting

confidence: 55%

“…2 Unfortunately, the only treatment for PE is still delivery, and PE causes iatrogenic preterm birth in about 15% of pregnancies. 3 PE may be life-threatening for both mother and child and is still a major cause of maternalfetal morbidity and mortality. After preeclamptic pregnancies, the mother may develop premature cardiovascular disease, such as chronic hypertension, ischemic heart disease, and stroke, later in life.…”

Section: Introductionmentioning

confidence: 99%

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Maternal serum perlecan and ischemia modified albumin levels as biomarkers of preeclampsia severity

Mokhtar

El-Hakam

Ebriheem

et al. 2022

EJI

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Preeclampsia (PE) is a pregnancy disorder characterized by hypertension and end-organ damage. Reliable biochemical markers for diagnosis and prediction of PE severity can improve maternal health, and several of these markers have been suggested till now. The goal of our study was to evaluate maternal serum levels of Perlecan and Ischemia modified albumin (IMA) in PE patients, and to investigate their relationship with the severity. This study included 45 pregnant women, who were divided into three groups: mild PE (n=15), severe PE (n=10), and normal pregnant females (n=20) as a control group. Maternal serum levels of Perlecan and IMA were determined by the enzyme linked immunosorbent assay (ELISA). Preeclamptic women with severe features have significantly higher serum Perlecan and IMA levels than women with mild PE and control (P<0.001 for both). Serum levels of Perlecan and IMA were significantly increased in patients with mild PE as compared with control (P<0.001 for both). Serum Perlecan levels were positively correlated with systolic blood pressure (SBP), diastolic blood pressure (DBP), ALT, AST, creatinine, urea, uric acid, and proteinuria, but negatively correlated with platelet count and fetal birth weight. Serum IMA level was positively correlated with SBP, DBP, but negatively correlated with Albumin, and fetal birth weight. The receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic performance of Perlecan and IMA in the prediction of PE severity. Serum Perlecan had greater sensitivity and lower specificity for severe PE than for mild PE. Serum IMA had greater sensitivity and lower specificity for severe PE than for mild PE. In conclusion, maternal serum Perlecan and IMA levels were biomarkers for monitoring PE and the increase in serum Perlecan levels was in accordance with the severity of PE. Also, Perlecan was superior to IMA as a predictor for PE severity.

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Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Maternal serum perlecan and ischemia modified albumin levels as biomarkers of preeclampsia severity

Mokhtar

El-Hakam

Ebriheem

et al. 2022

EJI

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“…Albumin is an important part of the antioxidant system and IMA formation is related to oxidative stress [ 14 ]. Within the hypoxic environment typical of the preeclamptic placenta, the N-terminal region of albumin has reduced capacity to bind to cobalt and this chemically changed albumin is referred to as IMA [ 14 , 20 ]. Currently, IMA is utilized as an established marker for ischemic heart diseases and approved by the US Food and Drug Authority (FDA) as the first biomarker with specific clinical application [ 35 – 38 ].…”

Section: Discussionmentioning

confidence: 99%

The diagnostic potential of oxidative stress biomarkers for preeclampsia: systematic review and meta-analysis

et al. 2022

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Background Preeclampsia is a multifactorial cardiovascular disorder of pregnancy. If left untreated, it can lead to severe maternal and fetal outcomes. Hence, timely diagnosis and management of preeclampsia are extremely important. Biomarkers of oxidative stress are associated with the pathogenesis of preeclampsia and therefore could be indicative of evolving preeclampsia and utilized for timely diagnosis. In this study, we conducted a systematic review and meta-analysis to determine the most reliable oxidative stress biomarkers in preeclampsia, based on their diagnostic sensitivities and specificities as well as their positive and negative predictive values. Methods A systematic search using PubMed, ScienceDirect, ResearchGate, and PLOS databases (1900 to March 2021) identified nine relevant studies including a total of 343 women with preeclampsia and 354 normotensive controls. Results Ischemia-modified albumin (IMA), uric acid (UA), and malondialdehyde (MDA) were associated with 3.38 (95% CI 2.23, 4.53), 3.05 (95% CI 2.39, 3.71), and 2.37 (95% CI 1.03, 3.70) odds ratios for preeclampsia diagnosis, respectively. The IMA showed the most promising diagnostic potential with the positive predictive ratio (PPV) of 0.852 (95% CI 0.728, 0.929) and negative predictive ratio (NPV) of 0.811 (95% CI 0.683, 0.890) for preeclampsia. Minor between-study heterogeneity was reported for these biomarkers (Higgins’ I2 = 0–15.879%). Conclusions This systematic review and meta-analysis identified IMA, UA, and MDA as the most promising oxidative stress biomarkers associated with established preeclampsia. IMA as a biomarker of tissue damage exhibited the best diagnostic test accuracy. Thus, these oxidative stress biomarkers should be further explored in larger cohorts for preeclampsia diagnosis. Graphical Abstract

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“…Thiols constitute a class of organic sulfur compounds characterized by the presence of a sulfhydryl functional group (–SH), also known as a thiol group [ 1 , 2 , 3 , 4 ]. In a biological system, thiols are present as albumin thiols, protein-bound thiols, and low-molecular-weight thiols such as cysteine (Cys), homocysteine (Hcy), glutathione (GSH), and cysteinylglycine (Cys-Gly) [ 2 , 4 , 5 , 6 ]. These free thiols are metabolically related [ 4 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Human Serum Albumin and Low-Molecular-Weight Thiols after Derivatization with Monobromobimane

2021

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Biothiols are extremely powerful antioxidants that protect cells against the effects of oxidative stress. They are also considered relevant disease biomarkers, specifically risk factors for cardiovascular disease. In this paper, a new procedure for the simultaneous determination of human serum albumin and low-molecular-weight thiols in plasma is described. The method is based on the pre-column derivatization of analytes with a thiol-specific fluorescence labeling reagent, monobromobimane, followed by separation and quantification through reversed-phase high-performance liquid chromatography with fluorescence detection (excitation, 378 nm; emission, 492 nm). Prior to the derivatization step, the oxidized thiols are converted to their reduced forms by reductive cleavage with sodium borohydride. Linearity in the detector response for total thiols was observed in the following ranges: 1.76–30.0 mg mL−1 for human serum albumin, 0.29–5.0 nmol mL−1 for α-lipoic acid, 1.16–35 nmol mL−1 for glutathione, 9.83–450.0 nmol mL−1 for cysteine, 0.55–40.0 nmol mL−1 for homocysteine, 0.34–50.0 nmol mL−1 for N-acetyl-L-cysteine, and 1.45–45.0 nmol mL−1 for cysteinylglycine. Recovery values of 85.16–119.48% were recorded for all the analytes. The developed method is sensitive, repeatable, and linear within the expected ranges of total thiols. The devised procedure can be applied to plasma samples to monitor biochemical processes in various pathophysiological states.

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The relationship between oxidative stress and preeclampsia. The serum ischemia-modified albumin levels and thiol/disulfide homeostasis

Cited by 18 publications

References 38 publications

Maternal serum perlecan and ischemia modified albumin levels as biomarkers of preeclampsia severity

Maternal serum perlecan and ischemia modified albumin levels as biomarkers of preeclampsia severity

The diagnostic potential of oxidative stress biomarkers for preeclampsia: systematic review and meta-analysis

Simultaneous Determination of Human Serum Albumin and Low-Molecular-Weight Thiols after Derivatization with Monobromobimane

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