The relationship between RAST and skin test results in patients with asthma or rhinitis: A quantitative study with purified major allergens

Witteman, A. M.; Stapel, S. O.; Perdok, Gerrard J.; Sjamsoedin, D.H.S.; Jansen, Henk M.; Aalberse, Rob C.; Zee, Jaring S. van der

doi:10.1016/s0091-6749(96)70278-6

Cited by 80 publications

(68 citation statements)

References 30 publications

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“…For a few sera, the antibody levels and biological activity were not associated (i.e. A2, A7, A29), but this phenomenon has already been described for several allergens including Der p 1 and Der p 2 [7,35,36]. Several factors such as the number and geometry of IgE epitopes as well as affinity/avidity of IgE binding may influence the mediator release activity.…”

Section: Discussionmentioning

confidence: 86%

“…HDM allergens are among the most common triggers of asthma and allergic rhinitis [3,4]. The frequency of IgE recognition, allergenic activity and clinical relevance of the major HDM allergens, Der p 1 and Der p 2, has been well investigated [5][6][7][8]. Both allergens are recognized by more than 90% of HDM-allergic patients and are considered as the major HDM allergens [5].…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy

Resch¹,

Weghofer²,

Seiberler³

et al. 2011

Clin Experimental Allergy

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Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy

Resch¹,

Weghofer²,

Seiberler³

et al. 2011

Clin Experimental Allergy

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“…Additionally, SPT have shown a high sensitivity but a low specificity for allergy diagnosis of asthma and rhinitis (Dreborg, 1989;Brand et al, 1993;Witteman et al, 1996;Bousquet et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Sensitization to profilin in the Central region of Portugal

Tavares

Machado

Loureiro

et al. 2008

Science of The Total Environment

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“…Skin tests were performed to determine the concentration of the allergen producing a 10–mm wheal response, 15 min after intracutaneous injection [18]. The AA group and ANA group underwent an allergen challenge with 1 ml of this allergen concentration brought to a final volume of 5 ml with saline, for each of the lung segments.…”

Section: Methodsmentioning

confidence: 99%

Influx of Neutrophils into the Airway Lumen at 4 h after Segmental Allergen Challenge in Asthma

Nocker¹,

Out²,

Weller³

et al. 1999

Int Arch Allergy Immunol

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Background: Segmental allergen challenge is a powerful tool to study inflammatory reactions in asthmatic airways. There is little information on the early events at 5 min and 4 h after allergen challenge with respect to the cell influx and the chemokine interleukin–8 (IL–8). Methods: Seven mild to moderate allergic asthmatics (AA group), 5 allergic nonasthmatics (ANA group) and 5 nonallergic controls underwent segmental allergen challenge, with allergen doses based upon skin reactivity. Bronchoalveolar lavage (BAL) samples were obtained before, 5 min and 4 h postchallenge, and were analyzed for cell numbers and differential counts, eosinophil and neutrophil chemotactic activity, and levels of IL–8. Results: At 5 min postchallenge, no changes were observed compared to baseline. At 4 h postchallenge, an increase was found in the number of neutrophils and the levels of IL–8, which was dependent on the dose of allergen in the AA and ANA group. At the same allergen dose, the increases in neutrophils and levels of IL–8 were calculated to be 91 and 67 times higher, respectively, in AA than in ANA. Levels of IL–8 correlated with the number of neutrophils and with the in vitro neutrophil chemotactic activities in BAL fluid. Conclusions: Neutrophil chemotactic activity is increased in BAL fluid at 4 h after segmental allergen challenge. We suggest that apart from IgE–mediated mast cell degranulation, additional local factors in the airways determine the degree of IL–8 increase and neutrophil influx.

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The relationship between RAST and skin test results in patients with asthma or rhinitis: A quantitative study with purified major allergens

Cited by 80 publications

References 30 publications

Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy

Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy

Sensitization to profilin in the Central region of Portugal

Influx of Neutrophils into the Airway Lumen at 4 h after Segmental Allergen Challenge in Asthma

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