The Relationship Between Stem Cell Seeding Efficiency and Position in Cell Cycle

Monette, Francis C.; Demello, John B.

doi:10.1111/j.1365-2184.1979.tb00123.x

Cited by 31 publications

(20 citation statements)

References 39 publications

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“…Similarly BERAN & TRIBUKAIT (1) described a lowering of seeding efficiency of control bone marrow in the spleens of irradiated mice pretreated by prolonged hypoxia, which is known to increase the capacity of self-renewal and generation of descendent cells determined 10 to 14 days later. These observations agree with the suggestion of MONETTE & DEMELLO (12) suggesting that colonyforming cells in cycle exhibit reduced spleen seeding when compared with normal marrow. One can hypothesize that the decrease of seeding efficiency as measured by the double-transplant method at 24 h after the injection of marrow cells is conditioned by the switching of transplanted CFUs into cell cycle induced by the metabolically influenced host environment.…”

Section: Discussionsupporting

confidence: 83%

Intermittent Feeding as A Factor Enhancing Hemopoietic Stem Cell Proliferation and Spleen Colony Formation in Irradiated Mice

Kozubı́k¹,

Pospı́šil²

1985

Acta Radiologica: Oncology

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The influence of metabolic stimulation induced by a 3 weeks' adaptation of the animals to intermittent food intake on hemopoietic stem cells was investigated in mice. The methods used included transplantation of bone marrow to lethally irradiated recipients, assay of CFUs number, seeding efficiency, and incorporation of '*'iododeoxyuridine into the DNA of spleen cells. A stimulatory effect of the metabolically influenced hemopoietic environment on the proliferative activity in stem cell compartments and on the recovery of hemopoietic organs was demonstrated. These stimulatory effects were most marked when the bone marrow of metabolically influenced donors was transplanted to similarly influenced recipients. .

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Section: Discussionsupporting

confidence: 83%

Intermittent Feeding as A Factor Enhancing Hemopoietic Stem Cell Proliferation and Spleen Colony Formation in Irradiated Mice

Kozubı́k¹,

Pospı́šil²

1985

Acta Radiologica: Oncology

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“…Furthermore, seeding efficiency is affected by the proliferative activity of stem cells. Monette and DeMello 37 showed that cycling spleen colony forming cells show a 50% reduction of their homing efficiency, while more recenty we showed that even a 3-h ex vivo incubation of bone marrow cells resulted in a similar 50% loss of seeding and consequently a 50% reduction of repopulating ability in a mouse model. 36 Stem cells with a stable retroviral vector integration have gone through a proliferative cycle during or shortly after the transfection protocol and consequently may have a seeding disadvantage in vivo as compared to the untransduced cells.…”

Section: Discussionmentioning

confidence: 58%

Stroma-conditioned medium and sufficient prestimulation improve fibronectin fragment-mediated retroviral gene transfer into human primitive mobilized peripheral blood stem cells through effects on their recovery and transduction efficiency

Breems

Driel

et al. 1998

Leukemia

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Mobilized peripheral blood stem cells (PBSC) are an attractive vehicle for cancer gene therapy. However these stem cells may have a reduced proliferative capacity due to previous cytotoxic chemotherapy treatment of the patient. In addition, primitive hematopoietic stem cells (HSC) from mobilized peripheral blood are almost exclusively quiescent, which makes it hard to induce proliferation in vitro and thus to improve stable transduction of introduced genes into a sufficiently large number of primitive stem cells. In this study CD34-selected mobilized PBSC from lymphoma and myeloma patients were used as target cells for retroviral-mediated gene transfer using a clinically relevant cell-and serum-free supernatant transduction protocol. We have investigated various parameters that may contribute to an improvement of the poor transduction efficiency of the primitive HSC, including prestimulation time, the use of the carboxy-terminal fibronectin fragment CH-296, as well as stromal cell line conditioned media. Retroviral supernatant transduction in combination with CH-296 increased significantly the gene transfer efficiency as compared to supernatant alone and made the use of polycations redundant. Gene transfer of primitive HSC (cobblestone area forming cell (CAFC) week 6) was specifically improved when this procedure was preceded by a 5-day pre-culture period as compared to a 2-day transduction procedure. However, irrespective of the numerical recovery, the CAFC week 6 after retroviral transduction produced less long-term culture colony-forming cells, suggesting a loss of individual stem cell quality. The addition of stroma-conditioned media during the pre-culture period did not affect the individual CAFC quality or transduction efficiency, but increased greatly the recovery of the total number of transduced and untransduced HSC leading to larger grafts containing higher numbers of transduced stem cells.

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“…HSCs that are in the G 0 phase of the cell cycle at the time of transplantation promote higher levels of engraftment than HSCs in the G 1 phase 45,46 and especially HSCs in the S phase. 47,48 A greater proportion of HSCs in old mice are cycling, 6 so this difference may underlie their diminished capability for homing. Third, it is possible there is an age-related change in radiosensitivity of HSCs manifested in the present experiments by differential ablation of host HSCs and thus a differential probability with which transplanted HSCs may find unoccupied niches.…”

Section: Discussionmentioning

confidence: 99%

Effects of aging on the homing and engraftment of murine hematopoietic stem and progenitor cells

Liang

Zant

Szilvassy

2005

Blood

347

265

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To test the hypothesis that aging has negative effects on stem-cell homing and engraftment, young or old C57BL/6 bone marrow (BM) cells were injected, using a limiting-dilution, competitive transplantation method, into old or young Ly5 congenic mice. Numbers of hematopoietic stem cells (HSCs) and progenitor cells (HPCs) recovered from BM or spleen were measured and compared with the numbers initially transplanted. Although the frequency of marrow competitive repopulation units (CRUs) increased approximately 2-fold from 2 months to 2 years of age, the BM homing efficiency of old CRUs was approximately 3-fold lower than that of young CRUs. Surprisingly, the overall size of individual stem-cell clones generated in recipients receiving a single CRU was not affected by donor age.

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The Relationship Between Stem Cell Seeding Efficiency and Position in Cell Cycle

Cited by 31 publications

References 39 publications

Intermittent Feeding as A Factor Enhancing Hemopoietic Stem Cell Proliferation and Spleen Colony Formation in Irradiated Mice

Intermittent Feeding as A Factor Enhancing Hemopoietic Stem Cell Proliferation and Spleen Colony Formation in Irradiated Mice

Stroma-conditioned medium and sufficient prestimulation improve fibronectin fragment-mediated retroviral gene transfer into human primitive mobilized peripheral blood stem cells through effects on their recovery and transduction efficiency

Effects of aging on the homing and engraftment of murine hematopoietic stem and progenitor cells

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