The relationship between the activation state of sucrose-phosphate synthase and the rate of CO2 assimilation in spinach leaves

Battistelli, Alberto; Adcock, Michael D.; Leegood, Richard C.

doi:10.1007/bf00194285

Cited by 29 publications

(15 citation statements)

References 9 publications

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“…Because direct product inhibition by sucrose-6-phosphate does not appear to play an important role in the regulation of SPS (Krause and Stitt 1992), it appears that SPS activity is a major factor in determining flux through the pathway of sucrose biosynthesis (Galtier et al 1993). Our results support the contention of a strong positive correlation between CO2 assimilation rate and SPS activity (Galtier et al 1993;Hurry et al 1994b) and SPS activation state (Battistelli et al 1991).…”

Section: Discussionsupporting

confidence: 89%

Feedback-limited photosynthesis and regulation of sucrose-starch accumulation during cold acclimation and low-temperature stress in a spring and winter wheat

Savitch

Gray

Hüner

1997

Planta

100

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Abstract. Regulation of sucrose-starch accumulation and its effect on CO2 gas exchange and electron transport were studied in low-temperature-stressed and coldacclimated spring (Katepwa) and winter (Monopol) cultivars of wheat (Triticum aestivum L.). Low-temperature stress of either the spring or winter cultivar was associated with feedback-limited photosynthesis as indicated by a 50-60% reduction in CO2 assimilation rates, twofold lower ATP/ADP ratio, and threefold lower electron transport rate than 20°C-grown control plants. However, no limitations were evident at the level of ribulose-l,5-bisphosphate carboxylase-oxygenase (Rubisco) in low-temperature-stressed plants. Cold acclimation of the spring cultivar resulted in similar feedback-limited photosynthesis observed during low-temperature stress. In contrast, cold acclimation of the winter cultivar resulted in an adjustment of CO2 assimilation rates to that of control plants. However, we show, for the first time, that this capacity to adjust CO2 assimilation still appeared to be associated with limited triose phosphate utilisation, a twofold lower ATP/ADP ratio, a reduction in electron transport rates but no restriction at the level of Rubisco compared to controls grown at 20°C. Thus, contrary to previous suggestions, we conclude that cold-acclimated Monopol appears to exhibit feedback limitations at the level of electron transport characteristic of cold-stressed plants despite the maintenance of high rates of CO2 assimilation. Furthermore, the differential capacity of the winter cultivar to adjust COa assimilation rates was associated with higher levels of sucrose accumulation and a threefold higher sucrose-phosphate synthase activity Abbreviations: AGPase = ADP-glucose pyrophosphorylase; FBPase = fructose-l,6-bisphosphatase; Fru 6-P -fructose 6-phosphate; Fru 1,6-BP = fructose 1,6-bisphosphate; Glc 6-P = glucose 6-phosphate; PGA = 3-phosphoglyceric acid; Rubisco -ribulose-1,5-bisphosphate carboxylase-oxygenase; RuBP = ribulose 1,5-bisphosphate; SPS = sucrose-phosphate synthase; Triose-P = triose phosphate

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Section: Discussionsupporting

confidence: 89%

Feedback-limited photosynthesis and regulation of sucrose-starch accumulation during cold acclimation and low-temperature stress in a spring and winter wheat

Savitch

Gray

Hüner

1997

Planta

100

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“…SPS activity has been shown to be a determinant of the synthetic rate of Suc in leaves (Battistelli et al, 1991;Winter and Huber, 2000;Baxter et al, 2003;Haigler et al, 2007). Interestingly, two lines of evidence from our study suggest a possible role of SnRK2.6 in increasing SPS activity.…”

Section: Snrk26 Imparts Carbon Source and Sink Strength Through Regusupporting

confidence: 58%

“…6 and 7). Because the capacity for Suc synthesis in the cytosol limits the maximal rates of photosynthesis by restricting the recycling rate of inorganic phosphate to support electron transport and carbon fixation in the chloroplast (Stitt, 1986), increasing Suc synthesis has a positive impact on photosynthetic rate (Ho and Thornley, 1978;Stitt, 1986;Battistelli et al, 1991;Baxter et al, 2003). In addition, it has been shown that the rate of Suc synthesis is also positively correlated with the rate of Suc export from leaves (Ho and Thornley, 1978;Rocher et al, 1989;Baxter et al, 2003;Park et al, 2007).…”

Section: Snrk26 Imparts Carbon Source and Sink Strength Through Regumentioning

confidence: 99%

The Protein Kinase SnRK2.6 Mediates the Regulation of Sucrose Metabolism and Plant Growth in Arabidopsis

et al. 2010

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In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal roles with the orthologous yeast Snf1 and mammalian AMP-activated protein kinase in modulating energy and metabolic homeostasis, the functional significance of the two plant-specific subfamilies SnRK2 and SnRK3 in these critical processes is poorly understood. We show here that SnRK2.6, previously identified as crucial in the control of stomatal aperture by abscisic acid (ABA), has a broad expression pattern and participates in the regulation of plant primary metabolism. Inactivation of this gene reduced oil synthesis in Arabidopsis (Arabidopsis thaliana) seeds, whereas its overexpression increased Suc synthesis and fatty acid desaturation in the leaves. Notably, the metabolic alterations in the SnRK2.6 overexpressors were accompanied by amelioration of those physiological processes that require high levels of carbon and energy input, such as plant growth and seed production. However, the mechanisms underlying these functionalities could not be solely attributed to the role of SnRK2.6 as a positive regulator of ABA signaling, although we demonstrate that this kinase confers ABA hypersensitivity during seedling growth. Collectively, our results suggest that SnRK2.6 mediates hormonal and metabolic regulation of plant growth and development and that, besides the SnRK1 kinases, SnRK2.6 is also implicated in the regulation of metabolic homeostasis in plants.Plants are constantly confronted by biotic and abiotic stresses and nutrient deprivation that disrupt metabolic and energy homeostasis or diminish carbon and energy availability for maintaining cell vitality, growth, and proliferation. It is believed that maintaining energy balance and availability at the cellular and organism levels is critical for optimizing plant growth and development. This underscores the cellular and physiological importance of energy sensors that control energy balance through regulating fundamental metabolic pathways in response to nutritional and environmental stresses.At present, a prevailing view is that energy sensors are evolutionarily conserved in eukaryotes, which are represented by Snf1 (for sucrose nonfermenting-1) in yeast, AMPK (for AMP-activated protein kinase) in

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“…Activity of SPS is closely correlated with the capacity of a leaf to synthesize sucrose (Harbron et aL, 1981;Huber & Israel, 1982) and is dependent on the assimilation rate (Battistelli, Adcock & Leegood, 1991); covalent tnodification alone could account for our results. Additionally, substances known to inhibit SPS may accumulate in diseased leaves.…”

Section: Discussionsupporting

confidence: 57%

Sucrose‐metabolizing enzymes from leaves of barley infected with brown rust (Puccinia hordei Otth.)

Tetlow

Farrar

1992

New Phytologist

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SUMMARYThe capacity for sucrose synthesis in primary leaves of barley {Hordeum vulgare L.) infected with brown rust {Puccinia hordei Otth.) was assessed by measuring the activity of sucrose phosphate synthase (SPS). SPS activity gradually declined during rust infection to 32 % of uninfected controls by the sporulation stage. Extractable SPS activity was constant in the light period in both uninfected and infected (sporulating) leaves; activity then declined in the first hour of darkness by 30 % in uninfected and 60 % in sporulating leaves until returning to the original activity at the start of the following light period. The activity of acid invertase (AI) increased more than four-fold in rust-infected (sporulating) primary leaves compared with controls. Extracellular AI activity in rusted tissue was two-fold greater than in healthy. The consequences of the changes in enzyme activities in the diseased plant are discussed in relation to export from the diseased leaf.

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The relationship between the activation state of sucrose-phosphate synthase and the rate of CO2 assimilation in spinach leaves

Cited by 29 publications

References 9 publications

Feedback-limited photosynthesis and regulation of sucrose-starch accumulation during cold acclimation and low-temperature stress in a spring and winter wheat

Feedback-limited photosynthesis and regulation of sucrose-starch accumulation during cold acclimation and low-temperature stress in a spring and winter wheat

The Protein Kinase SnRK2.6 Mediates the Regulation of Sucrose Metabolism and Plant Growth in Arabidopsis

Sucrose‐metabolizing enzymes from leaves of barley infected with brown rust (Puccinia hordei Otth.)

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